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Genetic Variation, Population Structure and the Possibility of Association Mapping of Biochemical and Agronomic Traits Using Dominant Molecular Markers in Iranian Tea Accessions

  • Mehdi RahimiEmail author
  • Mojtaba Kordrostami
  • Sanam SafaeiChaeikar
Research Paper
  • 4 Downloads
Part of the following topical collections:
  1. Biology

Abstract

One of the important methods for the study of quantitative traits is the association mapping through the use of phenotypic information and molecular markers. This study was carried out to examine the contrastive relationship between the biochemical and agronomic traits in tea accessions at the molecular level via association mapping and genetic diversity. In this study, 22 tea accessions were studied in an RCBD with two replications in 2017. Analysis of variance showed a significant difference between the accessions for the studied traits. Cluster analysis classified tea accessions into three groups based on the quantitative traits as well as DNA markers (SCoT and ISSR). Based on the Bayesian model, the tea accessions were classified into four subpopulations. In order to identify the molecular markers associated with the genes controlling traits variation, association mapping was carried out via two mixed linear models (MLM). Based on (G + P+K) and (G + P+Q + K) MLM models, 47 and 71 QTLs were identified for the studied traits, respectively. The SCoT4-1 locus was common between the four traits of chlorophyll a (CA), chlorophyll b (CB), total chlorophyll (CT) and leaf yield (LY) in both models. Also, the results showed that the development of STS and CAPS markers based on the identified markers can be used in breeding programs.

Keywords

Association analysis Chlorophyll Cluster ISSR and SCoT markers Mixed linear model 

Notes

Acknowledgements

We gratefully acknowledge the research funding provided for this project (No. 94802705) by Iran National Science Foundation (INSF) and Graduate University of Advanced Technology, Kerman, Iran.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Shiraz University 2019

Authors and Affiliations

  1. 1.Department of Biotechnology, Institute of Science and High Technology and Environmental SciencesGraduate University of Advanced TechnologyKermanIran
  2. 2.Department of Plant Biotechnology, Faculty of Agricultural SciencesUniversity of GuilanRashtIran
  3. 3.Tea Research Center, Horticultural Sciences Research InstituteAgricultural Research, Education and Extension Organization (AREEO)LahijanIran

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