Effect of Silver Nanoparticles on Growth of Wheat Under Heat Stress

  • Muhammad IqbalEmail author
  • Naveed Iqbal Raja
  • Zia-Ur-Rehman Mashwani
  • Mubashir Hussain
  • Muhammad Ejaz
  • Farhat Yasmeen
Research Paper


The present study was conducted to investigate the effect of AgNPs in the regulation of growth of wheat under heat stress. Plant extract of Moringa oleifera was used for AgNPs synthesis followed by characterization through UV–Vis spectroscopy, XRD, SEM and AFM. Different concentrations of AgNPs (25, 50, 75 and 100 mg/l) were applied to wheat plants at trifoliate stage. Heat stress was applied in range of 35–40 °C for 3 h/day for about 3 days. Exposure of heat stress alone reduced plant fresh mass (1.2%), dry mass (0.16%), root length (2.5%), shoot length (6.2%), root number (1.8%), leaf area (12.1%), leaf fresh mass (0.02%), leaf dry mass (0.01%) and leaf number (2%), respectively. However, application of AgNPs protects wheat plants against heat stress and improve plant root length (5 and 5.4%), shoot length (22.2 and 26.1%), root number (6.6 and 7.5%), plant fresh weight (1.3 and 2%) and plant dry weight (0.36 and 0.60%) in 50 and 75 mg/l AgNPs, respectively, compared to control. Similarly, remarkable increase in leaf area (18.3 and 33.8%), leaf number (4 and 4.8%), leaf fresh weight (0.09 and 0.15%) and leaf dry weight (0.06 and 0.18%) has been noticed in 50 and 75 mg/l AgNPs over respective value of control under heat stress. Although, AgNPs increase morphological growth at all tested combinations, but significant results were observed at 50 and 75 mg/l AgNPs under heat stress. In conclusion, application of AgNPs may protect wheat plants against heat stress by improving morphological growth.


Triticum aestivum Nanotechnology Silver nanoparticles Characterization Growth 


  1. Arokiyaraj S, Arasu MV, Vincent S (2014) Rapid green synthesis of silver nanoparticles from Chrysanthemum indicum and its antibacterial and cytotoxic effects: an in vitro study. Int J Nanomed 9:379–388CrossRefGoogle Scholar
  2. Arora S, Sharma P, Kumar S, Nayan R, Khanna PK, Zaidi MGH (2012) Gold-nanoparticle induced enhancement in growth and seed yield of Brassica juncea. Plant Growth Regul 66:303–310CrossRefGoogle Scholar
  3. Aslani F, Bagheri S, Julkapli NM, Juraimi AS, Hashemi FSG, Baghdadi A (2014) Effects of engineered nonmaterial’s on plants growth: an overview. Sci World J 6:22–28Google Scholar
  4. Asseng S, Foster I, Turner NC (2011) The impact of temperature variability on wheat yields. Glob Change Biol 17:997–1012CrossRefGoogle Scholar
  5. Aziz N, Faraz M, Pandey R, Shakir M, Fatma T, Varma A, Barman I, Prasad R (2015) Facile algae-derived route to biogenic silver nanoparticles: synthesis, antibacterial, and photocatalytic properties. Langmuir 31(42):11605–11612CrossRefGoogle Scholar
  6. Bao-shan L, Shao-qi D, Chun-hui L, Li-jun F, Shu-chun Q, Min Y (2004) Effect of TMS (nanostructured silicon dioxide) on growth of Changbai larch seedlings. J For Res 15:138–140CrossRefGoogle Scholar
  7. Barnabas B, Jager K, Feher A (2008) The effect of drought and heat stress on reproductive processes in cereals. Plant Cell Environ 31:11–38Google Scholar
  8. Chichiricco G, Poma A (2015) Penetration and toxicity of nanomaterials in higher plants. Nanomaterials 5(2):851–873CrossRefGoogle Scholar
  9. Ditta A (2012) How helpful is nanotechnology in agriculture? Adv Nat Sci Nanosci Nanotechnol 3(3):2–33CrossRefGoogle Scholar
  10. FAO (2014) FAOSTAT database. Accessed 15 May 2015
  11. Gopinath K, Gowri S, Karthika V, Arumugam A (2014) Green synthesis of gold nanoparticles from fruit extract of Terminalia arjuna, for the enhanced seed germination activity of Gloriosa superba. J Nanostruct Chem 4:1–11Google Scholar
  12. Gruyer N, Dorais M, Bastien C, Dassylva N, Triffault-Bouchet G (2013) Interaction between sliver nanoparticles and plant growth. In: international symposium on new technologies for environment control, energy-saving and crop production in greenhouse and plant factory-greensys, Jeju, Korea 6–11 Oct 2013Google Scholar
  13. Haghighi M, Afifipour Z, Mozafarian M (2012) The effect of N–Si on tomato seed germination under salinity levels. J Biol Environ Sci 6:87–90Google Scholar
  14. Hussain M, Raja NI, Mashwani ZR, Iqbal M, Sabir S, Yasmeen F (2017) In vitro seed germination and biochemical profiling of Artemisia absinthium exposed to various metallic nanoparticles. 3 Biotech 7(2):101–108CrossRefGoogle Scholar
  15. Iqbal M, Asif S, Ilyas N, Raja NI, Hussain M, Shabir S, Faz MNA, Rauf A (2016) Effect of plant derived smoke on germination and post germination expression of wheat (Triticum aestivum L.). Am J Plant Sci 7:806–813CrossRefGoogle Scholar
  16. Iravani S (2011) Green synthesis of metal nanoparticles using plants. Green Chem 13:2638–2650CrossRefGoogle Scholar
  17. Jaberzadeh A, Moaveni P, Moghadam HRT, Zahedi H (2013) Influence of bulk and nanoparticles titanium foliar application on some agronomic traits, seed gluten and starch contents of wheat subjected to water deficit stress. Not Bot Horti Agrobot 41:201–207CrossRefGoogle Scholar
  18. Joshi AK, Mishra B, Chatrath R, Ferrara GO, Singh RP (2007) Wheat improvement in India: present status, emerging challenges and future prospects. Euphytica 157:431–446CrossRefGoogle Scholar
  19. Kalteh M, Alipour ZT, Ashraf S, Aliabadi MM, Nosratabadi AF (2014) Effect of silica nanoparticles on basil (Ocimum basilicum) under salinity stress. J Chem Health Risks 4:49–55Google Scholar
  20. Khodakovskaya MV, Silva KD, Biris AS, Dervishi E, Villagarcia H (2012) Carbon nanotubes induce growth enhancement of tobacco cells. ACS Nano 6(3):2128–2135CrossRefGoogle Scholar
  21. Krishnaraj C, Jagan EG, Ramachandran R, Abirami SM, Mohan N, Kalaichelvan PT (2012) Effect of biologically synthesized silver nanoparticles on Bacopa monnieri (Linn.) Wettst. plant growth metabolism. Process Biochem 47(4):651–658CrossRefGoogle Scholar
  22. Kumar U, Joshi AK, Kumari M, Paliwal R, Kumar S, Roder MS (2010) Identification of QTLs for stay green trait in wheat (Triticum aestivum L.) in the ‘Chirya 3’ × ‘Sonalika’ population. Euphytica 174:437–445CrossRefGoogle Scholar
  23. Kumar V, Guleria P, Kumar V, Yadav SK (2013) Gold nanoparticle exposure induces growth and yield enhancement in Arabidopsis thaliana. Sci Total Environ 461:462–468CrossRefGoogle Scholar
  24. Li B, Tao G, Xie Y, Cai X (2012) Physiological effects under the condition of spraying nano SiO2 onto the Indocalamus barbatus McClure leaves. J Nanjing Univ (Natural Science Edition) 36:161–164Google Scholar
  25. Mishra V, Mishra RK, Dikshit A, Pandey AC (2014) Interactions of nanoparticles with plants: an emerging prospective in the agriculture industry. In: Ahmad P, Rasool S (eds) Emerging technologies and management of crop stress tolerance. Biological techniques, vol 1. Academic Press, Cambridge, pp 159–180CrossRefGoogle Scholar
  26. Modarresi M, Mohammdi V, Zali A, Mardi M (2010) Response of wheat yield and yield related traits of high temperature. Cereal Res Commun 38:23–31CrossRefGoogle Scholar
  27. Mondala S, Singha RP, Crossaa J, Huerta-Espinoa B, Sharmac I, Chatrathc R, Singhd GP, Sohue VS, Mavie GS, Sukaruf VSP, Kalappanavargg IK, Mishrah VK, Hussaini M, Gautamj NR, Uddink J, Barmak NCD, Hakimk A, Joshi AK (2013) Earliness in wheat: a key to adaptation under terminal and continual high temperature stress in South Asias. Field Crops Res 151:19–26CrossRefGoogle Scholar
  28. Monica RC, Cremonini R (2009) Nanoparticles and higher plants. Caryologia 62:161–165CrossRefGoogle Scholar
  29. Ngo QB, Dao TH, Nguyen HC, Tran XT, Nguyen TV, Khuu TD, Huynh TH (2014) Effects of nanocrystalline powders (Fe, Co and Cu) on the germination, growth, crop yield and product quality of soybean (Vietnamese species DT-51). Adv Nat Sci Nanosci Nanotechnol 5:15–23CrossRefGoogle Scholar
  30. Oćwieja M, Adamczyk Z (2014) Monolayers of silver nanoparticles obtained by chemical reduction methods. Surf Innov 2:160–172CrossRefGoogle Scholar
  31. Porter JR, Gawith M (1999) Temperature and the growth and development of wheat: a review. Eur J Agron 10:23–36CrossRefGoogle Scholar
  32. Prasad R, Kumar V, Prasad KS (2014) Nanotechnology in sustainable agriculture: present concerns and future aspects. Afr J Biotechnol 13(6):705–713CrossRefGoogle Scholar
  33. Rahman MM (2004) Response of wheat genotypes to late seeding heat stress. MS Thesis. Department of Crop Botany. Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh. pp 210Google Scholar
  34. Rahman LU, Qureshi R, Yasinzai MM, Shah A (2012) Synthesis and spectroscopic characterization of Ag–Cu alloy nanoparticles prepared in various ratios. Comptes Rendus Chim 15:533–538CrossRefGoogle Scholar
  35. Rai V, Acharya S, Dey N (2012) Implications of nanobiosensors in agriculture. J Biomater Nanobiotechnol 3:315–324CrossRefGoogle Scholar
  36. Raliya R, Tarafdar JC (2013) ZnO nanoparticle biosynthesis and its effect on phosphorous mobilizing enzyme secretion and gum contents in cluster bean (Cyamopsis tetragonoloba L.). Agric Res 2:48–57CrossRefGoogle Scholar
  37. Rauwel P, Kuunal S, Ferdov S, Rauwel E (2015) A review on the green synthesis of silver nanoparticles and their morphologies studied via TEM. Adv Mater Sci Eng 15:1–9Google Scholar
  38. Rezvani N, Sorooshzadeh A, Farhadi N (2012) Effect of nano-silver on growth of saffron in flooding stress. World Acad Sci Eng Technol 1:517–522Google Scholar
  39. Salama HMH (2012) Effects of silver nanoparticles in some crop plants, common bean (Phaseolus vulgaris L.) and corn (Zea mays L.). Int Res J Biotechnol 3:190–197MathSciNetGoogle Scholar
  40. Savithramma N, Ankanna S, Bhumi G (2012) Effect of nanoparticles on seed germination and seedling growth of Boswellia ovalifoliolata an endemic and endangered medicinal tree taxon. Nano Vis 2:61–68Google Scholar
  41. Scott N, Chen H (2003) Nanoscale science and engineering for agriculture and food systems. National Planning Workshop; November 18–19, 2002; Washington, DC.
  42. Semenov MA, Halford NG (2009) Identifying target traits and molecular mechanisms for wheat breeding under a changing climate. J Exp Bot 60:2791–2804CrossRefGoogle Scholar
  43. Shah M, Fawcett D, Sharma S, Tripathy SK, Poinern GEJ (2015) Green synthesis of metallic nanoparticles via biological entities. Materials 8:7278–7308CrossRefGoogle Scholar
  44. Sharma P, Jha AB, Dubey RS, Pessarakli M (2012) Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J Bot 12:134–136Google Scholar
  45. Siddiqui MH, Al-Whaibi MH, Faisal M, Al Sahli AA (2014) Nano-silicon dioxide mitigates the adverse effects of salt stress on Cucurbita pepo L. Environ Toxicol Chem 33:2429–2437CrossRefGoogle Scholar
  46. Singh VP, Srivastava PK, Prasad SM (2013) Nitric oxide alleviates arsenic induced toxic effects in ridged Luffa seedlings. Plant Physiol Biochem 71:155–163CrossRefGoogle Scholar
  47. Singh VP, Kumar J, Singh S, Prasad SM (2014) Dimethoate modifies enhanced UV-B effects on growth, photosynthesis and oxidative stress in Mung bean (Vigna radiata L.) seedlings: implication of salicylic acid. Pestic Biochem Physiol 116:13–23CrossRefGoogle Scholar
  48. Singh VP, Singh S, Kumar J, Prasad SM (2015) Hydrogen sulfide alleviates toxic effects of arsenate in pea seedlings through up-regulation of the ascorbate glutathione cycle: possible involvement of nitric oxide. J Plant Physiol 181:20–29CrossRefGoogle Scholar
  49. Syu YY, Hung JH, Chen JC, Chuang HW (2014) Impacts of size and shape of silver nanoparticles on Arabidopsis plant growth and gene expression. Plant Physiol Biochem 83:57–64CrossRefGoogle Scholar
  50. Trnka M, Dubrovsky M, Semeradova D, Zalud Z (2004) Projections of uncertainties in climate change scenarios into expected winter wheat yields. Theor Appl Climatol 77:229–249CrossRefGoogle Scholar
  51. Vinh NT, Paterson AH (2005) Genome mapping and its implication for stress resistance in plants. In: Ashraf M, Harris PJC (eds) Abiotic stresses: plant resistance through breeding and molecular approaches. CRC Press, Boca Raton, pp 15–23Google Scholar
  52. Wahid A (2007) Physiological implications of metabolites biosynthesis in net assimilation and heat stress tolerance of sugarcane (Saccharum officinarum) sprouts. J Plant Res 120:219–228CrossRefGoogle Scholar
  53. Wang X, Yang X, Chen S, Li Q, Wang W, Hou C, Gao X, Wang L, Wang S (2016) Zinc oxide nanoparticles affect biomass accumulation and photosynthesis in Arabidopsis. Front Plant Sci 6:1243Google Scholar
  54. Wrigley C (2006) Global warming and wheat quality. Cereal Foods World 51:34–36Google Scholar
  55. Yang F, Hong F, You W, Liu C, Gao F, Wu C, Yang P (2006) Influence of nano-anatase TiO2 on the nitrogen metabolism of growing spinach. Biol Trace Elem Res 110:179–190CrossRefGoogle Scholar
  56. Yasur J, Rani PU (2013) Environmental effects of nanosilver: impact on castor seed germination, seedling growth, and plant physiology. Environ Sci Pollut Res 20:8636–8648CrossRefGoogle Scholar
  57. Yin LY, Colman BP, McGill BM, Wright JP, Bernhardt ES (2012) Effects of silver nanoparticle exposure on germination and early growth of eleven wetland plants. PLoS One 7:7CrossRefGoogle Scholar
  58. You L, Rosegrant MW, Wood S, Sun D (2009) Impact of growing season temperature on wheat productivity in China. Agri Fores Meteorol 149(6–7):1009–1014CrossRefGoogle Scholar
  59. Zhao H, Dai T, Jiang D, Cao W (2008) Effects of high temperature on key enzymes involved in starch and protein formation in grains of two wheat cultivars. J Agron Crop Sci 194:47–54CrossRefGoogle Scholar

Copyright information

© Shiraz University 2017

Authors and Affiliations

  • Muhammad Iqbal
    • 1
    Email author
  • Naveed Iqbal Raja
    • 1
  • Zia-Ur-Rehman Mashwani
    • 1
  • Mubashir Hussain
    • 1
  • Muhammad Ejaz
    • 1
  • Farhat Yasmeen
    • 1
  1. 1.Department of BotanyPMAS Arid Agriculture UniversityRawalpindiPakistan

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