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Phytochemical Analysis, Antioxidant, Anticancer and Antibacterial Properties of the Caspian Sea Red Macroalgae, Laurencia caspica

  • Azam Moshfegh
  • Ali SalehzadehEmail author
  • Seyed Ataollah Sadat Shandiz
  • Mojdeh Shafaghi
  • Akram Sadat Naeemi
  • Soheil Salehi
Research Paper
  • 37 Downloads

Abstract

The current study was to investigate the gas chromatography/mass spectrometry, antioxidant, anticancer and antibacterial properties of red algae Laurencia caspica extract. The antioxidant capacity and total phenolic content of algae extract were measured utilizing DPPH scavenging assay and Folin–Ciocalteu procedure, respectively. Cell viability was measured using MTT assay against breast cancer T47D and HEK293 cells. Real-time PCR was used for measurement of gene expression. Flow cytometry was used for detecting of apoptosis in cells. Chemical analysis of L. caspica extract showed 22 most abundant components, and the most dominant compounds were Dodecane (17.33%), Undecane (6.616%) and Dodecane, 2, 6,11-trimethyl (5.483%). The real-time PCR results demonstrated dramatic elevation of mRNA levels of Nm23 in T47D cells. The Annexin V/PI staining showed that the L. caspica extract significantly increased apoptosis pathway in T47D cancer cell line. Moreover, antibacterial activity against pathogenic bacterial was evaluated. It seems that L. caspica has favorable resource with strong biomedical properties. So, further investigations were suggested to be carried out in cosmetics industries and food pharmaceutical.

Keywords

GC/MS Laurencia caspica Antioxidant Apoptosis Anticancer 

Notes

Acknowledgements

The authors are grateful to Dr. Mehdi Shafiee Ardestani for his support and contribution in the experimental study.

Compliance with Ethical Standards

Conflict of Interest

We confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome.

References

  1. Antolovich M, Prenzler PD, Patsalides E, McDonald S, Robards K (2002) Methods for testing antioxidant activity. Analyst 127:183–198CrossRefGoogle Scholar
  2. Bazzaz BSF, Khajehkaramadian M, Shokooheizadeh HR (2005) In vitro antibacterial activity of Rheum ribes extract obtained from various plant parts against clinical isolates of gram negative pathogens. Iran J Pharm Res 2:87–91Google Scholar
  3. Bevilacqua G, Sobel ME, Liotta LA, Steeg PS (1989) Association of low nm23 RNA levels in human primary infiltrating ductal breast carcinomas with lymph nodal involvement and other histopathological indicators of high metastatic potential. Cancer Res 49:5185–5190Google Scholar
  4. Bozdogan O, Yulug G, Vargel I, Cavusoglu T, Karabulut A, Karahan G (2015) Differential expression pattern of metastasis suppressor proteins in basal cell carcinoma. Int J Dermatol 54:905–915CrossRefGoogle Scholar
  5. Buxton IL, Yokdang N (2011) Extracellular Nm23 signaling in breast cancer: incommodus verum. Cancers 3:2844–2857CrossRefGoogle Scholar
  6. Crews P, Selover SJ (1986) Comparison the sesquiterpenes from the seaweed Laurencia pacifica and its epiphyte Erythrocystis saccata. Phytochemistry 25:1847–1852CrossRefGoogle Scholar
  7. Demirel Z, Yilmaz-Koz FF, Karabay-Yavasoglu NU, Ozdemir G, Sukata A (2011) Antimicrobial and antioxidant activities of solvent extracts and the essential oil composition of Laurencia obtusa and Laurencia obtusa var. pyramidata. Rom Biotech Lett 16:5927–5936Google Scholar
  8. Hay ME (2009) Marine chemical ecology: chemical signals and cues structure marine populations, communities, and ecosystems. Annu Rev Mar Sci 1:193–212CrossRefGoogle Scholar
  9. Howlett AR, Petersen OW, Steeg PS, Bissel MJ, Steeg PS (1994) A novel function for the nm23-Hl gene: overexpression in human breast carcinoma cells leads to the formation of basement membrane and growth arrest. J Natl Cancer Inst 86:1838–1844CrossRefGoogle Scholar
  10. Jung H, Seong HA, Ha H (2007) NM23-H1 tumor Suppressor and its interacting partner STRAP activate p53 function. J Biol Chem 282:35293–35307CrossRefGoogle Scholar
  11. Lin KH, Lin YW, Lee HF, Liu WL, Chen ST, Chang KS, Cheng SY (1995) Increased invasive activity of human hepatocellular carcinoma cells is associated with an overexpression of thyroid hormone beta 1 nuclear receptor and low expression of the anti-metastatic nm23 gene. Cancer Lett 98:89–95CrossRefGoogle Scholar
  12. Marinho-Soriano E, Fonseca PC, Carneiro MA, Moreira WSC (2006) Seasonal variation in the chemical composition of two tropical seaweeds. Bioresour Technol 97:2402–2406CrossRefGoogle Scholar
  13. Masuda M, Kawaguchi S, Abe T, Kawamoto T, Suzuki M (2002) Additional analysis of chemical diversity of the red algal genus Laurencia (Rhodomelaceae) from Japan. Phycol Res 50:135–144CrossRefGoogle Scholar
  14. Mohammadi M, Tajik H, Hajeb P (2013) Nutritional composition of seaweeds from the northern Persian Gulf. Iran J Fish Sci 12:232–240Google Scholar
  15. Pelegrin F, Robledo D (2013) Bioactive phenolic compounds from algae, in bioactive compounds from marine foods: plant and animal sources. In: Hernandes-Ledesma B, Herrero M, eds, John Wiley & Sons Ltd, Chichester.  https://doi.org/10.1002/9781118412893.ch6
  16. Prabhu VV, Siddikuzzaman VM, Grace C, Guruvayoorappn C (2012) Targeting tumor metastasis by regulating Nm23 gene expression. Asian Pac J Cancer Prev 13:3539–3548CrossRefGoogle Scholar
  17. Salehi S, Mirzaie A, Shandiz SAS, Noorbazargan H, Rahimi A, Yarmohammadi S, Ashrafi F (2016) Chemical composition, antioxidant, antibacterial and cytotoxic effects of Artemisia marschalliana Sprengel extract. Nat Prod Res 31(4):469–472CrossRefGoogle Scholar
  18. Shandiz SAS, Khosravani M, Mohammadi S, Noorbazargan H, Mirzaie A (2016a) Evaluation of imatinib mesylate (Gleevec) on KAI1/CD82 gene expression in breast cancer MCF-7 cells using quantitative real-time PCR. Asian Pac J Trop Biomed 6:159–163CrossRefGoogle Scholar
  19. Shandiz SAS, Farasati S, Saeedi B, Baghbani-Aarani F, Akbari AE, Keshavarz-Pakseresht B (2016b) Up regulation of KAI1 gene expression and apoptosis effect of imatinib mesylate in gastric adenocarcinoma (AGS) cell line. Asian Pac J Trop Dis 6:120–125CrossRefGoogle Scholar
  20. Shangyang SH, Xu B, He M, Lan X, Wang Q (2010) Nm23-H1 suppresses hepatocarcinoma cell adhesion and migration on fibronectin by modulating glycosylation of integrin beta 1. J Exp Clin Cancer Res 29:93CrossRefGoogle Scholar
  21. Siegel R, Ma J, Zou ZH, Jemal A (2014) Cancer statistics 2014. CA Cancer J Clin 64:9–29CrossRefGoogle Scholar
  22. Steeg PS, Bevilacqua G, Kopper L (1988) Evidence for a novel gene associated with low tumor metastatic potential. J Natl Cancer Inst 80:200–204CrossRefGoogle Scholar
  23. Stein E, Andreguetti DX, Rocha CS, Fujii MT, Baptista M, Colepicolo P, Indig GL (2011) Search for cytotoxic agents in multiple Laurencia complex seaweed species (Ceramiales, Rhodophyta) harvested from the Atlantic Ocean with emphasis on the Brazilian state of espiririto santo. Revista Brasileira de Farmacognosia. 21:239–243CrossRefGoogle Scholar
  24. Su H, Shi DY, Li J, Guo SJ, Li LL, Yuan ZH, Zhu XB (2009) Sesquiterpenes from Laurencia similis. Molecules 14:1889–1897CrossRefGoogle Scholar
  25. Umamaheswari A, Shreevidya R, Nuni A (2008) In vitro antibacterial activity of Bougainvillea spectabilis leaves extracts. Adv Biol Res 2:1–5CrossRefGoogle Scholar
  26. Yoshida BA, Sokoloff MM, Welch DR, Rinker-Shaeffer CW (2000) Metastasis-suppresor Genes: a review and perspective on an emerging field. J Natl Inst 92:1717–1730CrossRefGoogle Scholar
  27. Zaleta-Pinet DA, Holland IP, Muñoz-Ochoa M, Murillo-Alvarez JI, Sakoff JA, Altena IA, Mc Cluskey A (2014) Cytotoxic compounds from Laurencia pacifica. Org Med Chem Lett 4:8CrossRefGoogle Scholar
  28. Zhang WW, Duan XJ, Huang HL, Zhang Y, Wang BG (2007) Evaluation of 28 marine algae from the Qingdao coast for anti-oxidative capacity and determination of antioxidant efficiency and total phenolic content of fractions and subfractions derived from Symphyocladia latiuscula (Rhodomelaceae). J Appl Phycol 19:97–108CrossRefGoogle Scholar

Copyright information

© Shiraz University 2017

Authors and Affiliations

  1. 1.Department of BiologyLahijan Branch, Islamic Azad UniversityLahijanIran
  2. 2.Department of BiologyRasht Branch, Islamic Azad UniversityRashtIran
  3. 3.Department of BiologyCentral Tehran Branch, Islamic Azad UniversityTehranIran
  4. 4.Department of Biology, Faculty of ScienceUniversity of GuilanRashtIran
  5. 5.Department of Phytochemistry and Essential Oils Technology, Faculty of Pharmaceutical ChemistryPharmaceutical Sciences Branch, Islamic Azad University (IAUPS)TehranIran

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