Advertisement

Albendazole and Praziquantel Chitosan Nanoparticles: Preparation, Characterization, and In Vitro Release Study

  • Negin Torabi
  • Faramarz Dobakhti
  • Ali Haniloo
Research Paper

Abstract

Albendazole (ABZ) and praziquantel (PZQ) are two drugs with anthelminthic activity, but their low solubility in biological fluid and subsequently poor bioavailability after oral administration confine their clinical usage. The aim of this study was to prepare chitosan nanoparticles loaded with either ABZ or PZQ as new formulations for improvement of their bioavailability. In order to synthesis nanoparticles, the chitosan powders with different molecular weights were added to acetic acid solution containing Poloxamer 407. To optimize the method, a number of parameters were explored by changing one parameter while others were kept constant. Particle size, polydispersity index, ξ-potential, drug loading, and in vitro drug release were measured. The average sizes of chitosan nanoparticles loaded with ABZ and PZQ were 224.9 and 174.6, respectively. Drug-loading and entrapment efficiencies were measured to be 0.55 and 11% for ABZ, respectively, while these were 0.53 and 22% for PZQ, respectively. The results indicated that chitosan molecular weight, chitosan concentration, chitosan-to-sodium tripolyphosphate ratio, and solution pH values were all influential on the size of the nanoparticles. It seems that the current procedure is suitable for making chitosan nanoparticles containing PZQ, although not suitable enough for ABZ chitosan nanoparticles.

Keywords

Chitosan Nanoparticles Albendazole Praziquantel Ionotropic gelation 

Notes

Acknowledgements

This study is a part of the Ph.D. thesis (ZUMS.REC.1393.177) and was financially supported by Zanjan University of Medical Sciences. The authors wish to thank Mrs. Mina Mohammadi and Miss Zahra Karami for their technical assistance.

Compliance with ethical standards

Conflict of interest

The authors have no conflicts of interest to declare.

References

  1. Benz GW, Ernst JV (1977) Anthelmintic activity of albendazole against gastrointestinal nematodes in calves. Am J Vet Res 38:1425–1426Google Scholar
  2. Bharat C, Tyagi S, Chirag P, Pinkesh P, Jaimin P, Kumar U, Mangukia D (2013) Preparation and evaluation of nanosuspension of poorly soluble drug albendazole. J Drug Discov Ther 1:37–42Google Scholar
  3. Bhattarai N, Gunn J, Zhang M (2010) Chitosan-based hydrogels for controlled, localized drug delivery. Adv Drug Deliv Rev 62:83–99. doi: 10.1016/j.addr.2009.07.019 CrossRefGoogle Scholar
  4. Csaba N, Koping-Hoggard M, Alonso MJ (2009) Ionically crosslinked chitosan/tripolyphosphate nanoparticles for oligonucleotide and plasmid DNA delivery. Int J Pharm 382:205–214. doi: 10.1016/j.ijpharm.2009.07.028 CrossRefGoogle Scholar
  5. Darvishi MH, Nomani A, Amini M, Shokrgozar MA, Dinarvand R (2013) Novel biotinylated chitosan-graft-polyethyleneimine copolymer as a targeted non-viral vector for anti-EGF receptor siRNA delivery in cancer cells. Int J Pharm 456:408–416. doi: 10.1016/j.ijpharm.2013.08.069 CrossRefGoogle Scholar
  6. des Rieux A, Fievez V, Garinot M, Schneider YJ, Preat V (2006) Nanoparticles as potential oral delivery systems of proteins and vaccines: a mechanistic approach. J Control Release 116:1–27. doi: 10.1016/j.jconrel.2006.08.013 CrossRefGoogle Scholar
  7. Dvoroznakova E, Hrckova G, Boroskova Z, Velebny S, Dubinsky P (2004) Effect of treatment with free and liposomized albendazole on selected immunological parameters and cyst growth in mice infected with Echinococcus multilocularis. Parasitol Int 53:315–325. doi: 10.1016/j.parint.2004.05.001 CrossRefGoogle Scholar
  8. Fan W, Yan W, Xu ZS, Ni H (2012) Formation mechanism of monodisperse, low molecular weight chitosan nanoparticles by ionic gelation technique. Colloid Surf B 90:21–27. doi: 10.1016/j.colsurfb.2011.09.042 CrossRefGoogle Scholar
  9. Filippou D, Tselepis D, Filippou G, Papadopoulos V (2007) Advances in liver echinococcosis: diagnosis and treatment. Clin Gastroenterol H 5:152–159. doi: 10.1016/j.cgh.2006.08.017 CrossRefGoogle Scholar
  10. Hamidi M, Azadi A, Rafiei P (2008) Hydrogel nanoparticles in drug delivery. Adv Drug Deliv Rev 60:1638–1649. doi: 10.1016/j.addr.2008.08.002 CrossRefGoogle Scholar
  11. Janes KA, Alonso MJ (2003) Depolymerized chitosan nanoparticles for protein delivery: Preparation and characterization. J Appl Polym Sci 88:2769–2776. doi: 10.1002/app.12016 CrossRefGoogle Scholar
  12. Kang BS, Lee SE, Ng CL, Kim JK, Park JS (2015) Exploring the preparation of albendazole-loaded chitosan-tripolyphosphate nanoparticles. Materials 8:486–498. doi: 10.3390/ma8020486 CrossRefGoogle Scholar
  13. Lehr CM, Bouwstra JA, Schacht EH, Junginger HE (1992) In vitro evaluation of mucoadhesive properties of chitosan and some other natural polymers. Int J Pharm 78:43–48CrossRefGoogle Scholar
  14. Leonardi D, Lamas MC, Olivieri AC (2008) Multiresponse optimization of the properties of albendazole–chitosan microparticles. J Pharm Biomed Anal 48:802–807. doi: 10.1016/j.jpba.2008.08.006 CrossRefGoogle Scholar
  15. Letchford K, Burt H (2007) A review of the formation and classification of amphiphilic block copolymer nanoparticulate structures: micelles, nanospheres, nanocapsules and polymersomes. European J Pharm Biopharm: Off J Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik eV 65:259–269. doi: 10.1016/j.ejpb.2006.11.009 CrossRefGoogle Scholar
  16. Lin SY, Kawashima Y (1985) The influence of three poly(oxyethylene)poly(oxypropylene) surface-active block copolymers on the solubility behavior of indomethacin. Pharm Acta Helv 60:339–344Google Scholar
  17. Liu Y et al (2013) Novel albendazole-chitosan nanoparticles for intestinal absorption enhancement and hepatic targeting improvement in rats. J Biomed Mater Res B 101B:998–1005. doi: 10.1002/jbm.b.32908 CrossRefGoogle Scholar
  18. Min KH et al (2008) Hydrophobically modified glycol chitosan nanoparticles-encapsulated camptothecin enhance the drug stability and tumor targeting in cancer therapy. J Control Release 127:208–218. doi: 10.1016/j.jconrel.2008.01.013 CrossRefGoogle Scholar
  19. Paredes AJ, Llabot JM, Sanchez Bruni S, Allemandi D, Palma SD (2016) Self-dispersible nanocrystals of albendazole produced by high pressure homogenization and spray-drying. Drug Dev Ind Pharm 42(10):1564–1570. doi: 10.3109/03639045.2016.1151036 CrossRefGoogle Scholar
  20. Pourgholami MH, Akhter J, Wang L, Lu Y, Morris DL (2005) Antitumor activity of albendazole against the human colorectal cancer cell line HT-29: in vitro and in a xenograft model of peritoneal carcinomatosis. Cancer Chemother Pharmacol 55:425–432. doi: 10.1007/s00280-004-0927-6 CrossRefGoogle Scholar
  21. Rampino A, Borgogna M, Blasi P, Bellich B, Cesaro A (2013) Chitosan nanoparticles: preparation, size evolution and stability. Int J Pharm 455:219–228. doi: 10.1016/j.ijpharm.2013.07.034 CrossRefGoogle Scholar
  22. Ravichandran R (2010) Preparation and characterization of albendazole nanosuspensions for oral delivery. Int J Green Nanotechnol Biomed 2:B1–24Google Scholar
  23. Sashiwa H, Aiba SI (2004) Chemically modified chitin and chitosan as biomaterials. Prog Polym Sci 29:887–908. doi: 10.1016/j.progpolymsci.2004.04.001 CrossRefGoogle Scholar
  24. Shu XZ, Zhu KJ (2002) Controlled drug release properties of ionically cross-linked chitosan beads: the influence of anion structure. Int J Pharm 233:217–225. doi: 10.1016/S0378-5173(01)00943-7 CrossRefGoogle Scholar
  25. Vila A, Sanchez A, Janes K, Behrens I, Kissel T, Jato JLV, Alonso MJ (2004) Low molecular weight chitosan nanoparticles as new carriers for nasal vaccine delivery in mice. Eur J Pharm Biopharm 57:123–131. doi: 10.1016/j.ejpb.2003.09.006 CrossRefGoogle Scholar
  26. Vogt M, Kunath K, Dressman JB (2008) Dissolution improvement of four poorly water soluble drugs by cogrinding with commonly used excipients. Eur J Pharm Biopharm 68:330–337. doi: 10.1016/j.ejpb.2007.05.009 CrossRefGoogle Scholar

Copyright information

© Shiraz University 2017

Authors and Affiliations

  1. 1.Department of Medical Parasitology and Mycology, School of MedicineZanjan University of Medical SciencesZanjanIran
  2. 2.School of PharmacyZanjan University of Medical SciencesZanjanIran

Personalised recommendations