Kinetic of α-Amylase and Comparison its Inhibition by Ethanol and Aqueous Extracts of Otostegia persica, Salvia mirzayanii and Zataria multiflora

  • Soheila Moein
  • Samaneh Jahanshai
  • Mahsa Rahimzadeh
  • Mahmoodreza Moein
Research Paper
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Abstract

Three plants namely, Otostegia persica, Salvia mirzayanii and Zataria multiflora are used as antidiabetic agents in the traditional medicine of Hormozgan and Fars provinces, Iran. In this study, the inhibition of α-amylase by the ethanol extracts and fractions of aqueous extracts of these plants was investigated. To measure the enzyme inhibition, 5 and 20 mg/mL of the extracts, and to measure kinetic properties of the enzyme, 5 mg/mL of the extracts, were incubated with 0.5 % of starch (as substrate) and 20 µL of the enzyme. Kinetics studies of α-amylase showed that Km of the enzyme in Otostegia persica and Salvia mirzayanii in comparison with different concentrations of acarbose (0.5, 1 and 2 µM), increased and decreased, respectively. Km of the enzyme in the presence of Zataria multiflora in comparison with 2 µM of acarbose increased and in comparison with the other concentrations of acarbose (0.5 and 1 µM), it decreased. The inhibition of α-amylase by Otostegia persica, Salvia mirzayanii and Zataria multiflora was competitive, mixed and noncompetitive, respectively. The inhibition of α-amylase by acarbose was noncompetitive. Ethanol extracts of Otostegia persica (96.7 ± 1.05 %) and Zataria multiflora (59 ± 6.8 %), and water–methanol fractions of Salvia mirzayanii (94.8 ± 2.5 %) and Zataria multiflora (93.4 ± 2.2 %) had significant inhibitory effects on α-amylase, respectively.

Keywords

Diabetes α-Amylase inhibition Otostegia persica Zataria multiflora Salvia mirzayanii 
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Notes

Acknowledgments

The authors thank Hormozgan University of Medical Sciences (Grant No. 9316) and Shiraz University of Medical Sciences (Grant No. 8672) for the grants.

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Copyright information

© Shiraz University 2016

Authors and Affiliations

  • Soheila Moein
    • 1
    • 2
  • Samaneh Jahanshai
    • 3
  • Mahsa Rahimzadeh
    • 1
    • 2
  • Mahmoodreza Moein
    • 4
    • 5
  1. 1.Molecular Medicine Research CenterHormozgan University of Medical SciencesBandar AbbasIran
  2. 2.Department of Biochemistry, Faculty of MedicineHormozgan University of Medical SciencesBandar AbbasIran
  3. 3.Department of Biology, College of Agriculture and Basic SciencesPayame Noor UniversityMashhadIran
  4. 4.Medicinal Plants Processing Research CenterShiraz University of Medical SciencesShirazIran
  5. 5.Department of Pharmacognosy, School of PharmacyShiraz University of Medical SciencesShirazIran

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