Blockage of HOTAIR Reduced Cell Proliferation in Human Ovarian Cancer Cells Through Upregulation of AKT2
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Ovarian cancer (OC) is the most leading cause of cancer-related death in women. lncRNA HOTAIR is emerging as an onco-lncRNA that its overexpression provokes progression and metastasis in OC. In this study, we focused on evaluating the efficiency of antisense-based HOTAIR inhibition in OC tumor cells death.
We transfected antisense LNA GapmeR into OC cell line (SKOV3). Then, the survival rates of SKOV3 were measured by MTT at all three time points after transfection. Moreover, we examined the expression level of AKT2 in SKOV3 under the influence of GapmeRs using qPCR at 24, 48 and 72 h after transfection.
The viability of SKOV3 was significantly reduced over time under the suppression of lncRNA HOTAIR. The qPCR results disclosed the remarkable increase in AKT2 expression level via inhibition of HOTAIR in SKOV3 cell line.
Our data imply that inhibition of HOTAIR by antisense LNA GapmeR induced OC cell death. Hence, we concluded that antisense-based strategies might be considered as a potential therapeutic approach in OC treatment.
KeywordsOvarian cancer Long non-coding RNA lncRNA HOTAIR Cell death
This study was conducted with the support of Isfahan University of Medical Science Bushehr Branch, Islamic Azad University (Iran).
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 2.Chen VW, Ruiz B, Killeen JL, Coté TR, Wu XC, Correa CN, et al. Pathology and classification of ovarian tumors. Cancer Interdiscip Int J Am Cancer Soc. 2003;97(S10):2631–42.Google Scholar
- 4.Mutch DG, editor. Surgical management of ovarian cancer. Seminars in oncology. Amsterdam: Elsevier; 2002.Google Scholar
- 15.Yiwei T, Hua H, Hui G, Mao M, Xiang L. HOTAIR interacting with MAPK1 regulates ovarian cancer skov3 cell proliferation, migration, and invasion. Med Sci Monit Int Med J Exp Clin Res. 2015;21:1856.Google Scholar