The population of Sclerotinia sclerotiorum affecting common bean in Brazil is structured by mycelial compatibility groups
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The genetic structure of a population of Sclerotinia sclerotiorum causing white mold on common bean in Brazil was studied using microsatellite (SSR) loci and mycelial compatibility groups (MCGs). A total of 300 isolates were analyzed and 154 SSR haplotypes and 32 MCGs were identified. Two MCGs were widely distributed and accounted for 70% of the isolates. Six SSR haplotypes were associated with more than one closely related MCG. There was no evidence of random association of alleles among loci when the population comprised by all MCGs was analyzed, suggesting that outcrossing is absent or rare. Nevertheless, there was evidence of random mating within the major MCGs. Seven genetic groups were identified, one of them comprising only highly pigmented isolates. Isolates of distinct MCGs did not differ in virulence. There was strong genetic differentiation among MCGs: more than 95% of the total genetic variation was attributed to differences among these groups. Therefore, MCGs contribute to the structure of the population of S. sclerotiorum in Brazil.
KeywordsWhite mold Genetics Variability Resistance Phaseolus vulgaris
The authors were supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq. This research was supported by FAPEMIG and CNPq. The authors thank Dr. Antônio Félix da Costa, Dr. Hélcio Costa and Airton Luiz Pazinato for sampling of S. sclerotiorum isolates in Pernambuco, Espírito Santo and São Paulo state, respectively. The authors thank Dr. Linda Kohn for sending DNA of S. sclerotiorum isolate LMK211.
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