Periwinkle proliferation disease associated with 16SrI-B phytoplasma in Mexico
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Catharantus roseus, known as periwinkle, is highly susceptible to phytoplasma infection. Periwinkle plants showing proliferation symptoms were detected during 2013–2014 in four geographically distant states in Mexico. The presence of phytoplasmas was confirmed through the amplification of 16S F2nR2 and cpn60 UT sequences from symptomatic plants. Sequencing, phylogenetic analysis and in vitro RFLP revealed that the isolates were ‘Candidatus Phytoplasma asteris’-related strains and members of the 16SrI-B subgroup, confirming the association of this phytoplasma group with periwinkle proliferation disease in Mexico. We also demonstrated that the use of the approximately 550 pb cpn60 universal target sequences allow the differentiation of two 16SrI-B strains, designated here as MePP-Centre, and MePP-South.
Keywords16SrI-B ‘Candidatus phytoplasma asteris’ Catharanthus cpn60 UT Mexican periwinkle proliferation
We thank Christine Hammond and Jennifer Town for their support in lab work. This work was supported by the Genomic Research and Development Initiative for the shared priority project on quarantine and invasive species. Edel Pérez-López thanks CONACYT for a PhD scholarship (CVU: 517835).
- Bonfield JK, Whitwham A (2010) Gap5 - editing the billion fragment sequence assembly. Bioinformatics 26:1699–1703Google Scholar
- Caicedo JD, Rivera-Vargas LI, Segarra AE, Davis RE (2015) Detection and molecular characterisation of a group 16SrIX phytoplasma infecting citrus (Citrus sinensis and C. limon), coffee (Coffea arabica), periwinkle (Catharanthus roseus) and tabebuia (Tabebuia heterophylla) in Puerto Rico. Australas Plant Dis Notes 10:28Google Scholar
- Choi YH, Tapias EC, Kim HK, Lefeber AW, Erkelens C, Verhoeven JT, Brzin J, Zel J, Verpoorte R (2004) Metabolic discrimination of Catharanthus roseus leaves infected by phytoplasma using 1 H-NMR spectroscopy and multivariate data analysis. Plant Physiol 135:2398–2410CrossRefPubMedPubMedCentralGoogle Scholar
- Contaldo N, Bertaccini A, Paltrinieri S, Windsor HM, Windsor DG (2012) Axenic culture of plant pathogenic phytoplasmas. Phytopathol Mediterr 51:607–617Google Scholar
- Davis RE, Zhao Y, Dally EL, Lee I-M, Jomantiene R, Douglas SM (2013) ‘Candidatus Phytoplasma pruni’, a novel taxon associated with X-disease of stone fruits, Prunus spp.: multilocus characterization based on 16SrRNA, secY, and ribosomal protein genes. Int J Syst Evol Microbiol 63:766–776CrossRefPubMedGoogle Scholar
- FAO (2012) FAOSTAT, Production. Available at: http://faostat.fao.org/site/567/DesktopDefault.aspx? PageID=567#ancor. Accessed 28 Aug 2015
- Gundersen DE, Lee I-M (1996) Ultrasensitive detection of phytoplasma by nested-PCR assays using two universal primer pairs. Phytopathol Mediterr 35:114–151Google Scholar
- Hodgetts J, Crossley D, Dickinson M (2014) Techniques for the maintenance and propagation of phytoplasmas in glasshouse collections of Catharanthus roseus. In: Dickinson M, Hodgetts J (eds) Phytoplasma: methods and protocols. Humana Press Inc., New YorkGoogle Scholar
- Nejat N, Sijam K, Abdullah SNA, Vadamalai G, Dickinson M (2010) Molecular characterization of an aster yellows phytoplasma associated with proliferation of periwinkle in Malaysia. Afr J Biotechnol 9:2305–2315Google Scholar
- Pérez-López E, Dumonceaux TJ, Olivier CY, Luna-Rodríguez M (2014) Identification of ‘Candidatus phytoplasma phoenicium’ in periwinkle from Cuba. Rev Mex Fitopatol 32:S47Google Scholar
- Torres L, Galdeano E, Docampo D, Conci L (2004) Characterization of an aster yellows phytoplasma associated with Catharanthus little leaf in Argentina. J Plant Pathol 86:209–214Google Scholar