Tropical Plant Pathology

, Volume 41, Issue 3, pp 193–197 | Cite as

Identification and characterization of Ditylenchus spp. populations from garlic in New York State, USA

  • Sarah J. Pethybridge
  • Adrienne Gorny
  • Traci Hoogland
  • Lisa Jones
  • Frank Hay
  • Christine Smart
  • George Abawi
Short Communication


Ditylenchus dipsaci is an important pathogen of onion and garlic and causes reductions in bulb quality. Between 2012 and 2014, 345 garlic bulb samples were tested for Ditylenchus spp. The average incidence of Ditylenchus spp. was 14.1 %. Twenty-five individuals from 31 Ditylenchus spp. populations were selected for identification by morphology, species-specific PCR, and sequencing of the ITS1-5.8S-ITS2 region. Thirty populations were identified by morphology and species-specific PCR as D. dipsaci. Sequencing of the ITS1-5.8S-ITS2 region of the ribosomal DNA found the populations were 99.7 to 100 % similar to each other, and 99.3 to 100 % similar to other D. dipsaci reference isolates from France, and California, USA. One population was identified as Ditylenchus sp. and was 97 % similar within the ITS1-5.8S-ITS2 region to D. destructor. This information will be useful for the monitoring of future pest outbreaks in garlic in New York State, USA, and the effectiveness of management recommendations.


Ditylenchus D. dipsaci D. destructor Garlic Stem and bulb nematode 



This project was supported by the United States Department of Agriculture Specialty Crop program through the New York State Department of Agriculture and Markets (C200756), and the United States Department of Agriculture, National Institute of Food and Agriculture (USDA-NIFA) Hatch project NYG-625424 managed by The New York Agricultural Experiment Station, Cornell University, Geneva, NY, USA. Thanks also to Christy Hoepting, Robert Hadad, and Crystal Stewart, Cornell Cooperative Extension, NY, USA for coordinating sampling, and to Kundan Moktan, Cornell University for initial processing of samples.


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Copyright information

© Sociedade Brasileira de Fitopatologia 2016

Authors and Affiliations

  1. 1.Cornell University, School of Integrative Plant Science, Section of Plant Pathology and Plant-Microbe BiologyCornell UniversityGenevaUSA
  2. 2.The Department of Plant Sciences and Plant PathologyMontana State UniversityBozemanUSA
  3. 3.Department of Botany and Plant PathologyOregon State UniversityCorvallisUSA

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