Tropical Plant Pathology

, Volume 40, Issue 5, pp 345–349 | Cite as

Identification of natural hosts of Zucchini lethal chlorosis virus

  • Viviana M. Camelo-García
  • Élison F. B. Lima
  • Jorge A. M. RezendeEmail author
Short Communication


Zucchini lethal chlorosis virus (ZLCV) is a species of the genus Tospovirus, in the family Bunyaviridae, and is transmitted by thrips (Frankliniella zucchini). To date, ZLCV has only been reported in Brazil, where it causes a disease known as lethal chlorosis of zucchini squash. The aim of this study was to identify additional species susceptible to ZLCV through natural infection in the field and to provide subsidies for the establishment of management strategies for the disease. Six hundred fifty-three samples belonging to 44 plant species representing 13 families were analyzed using PTA-ELISA. ZLCV naturally infected the following species: Cucumis anguria (1/12), Datura stramonium (1/59) and Luffa aegyptiaca (1/3). This work provides evidence that the main natural hosts of this tospovirus are concentrated in the cucurbit family. Presently, the management of inoculum sources can minimize the damage caused by this tospovirus.


Tospovirus Curcubitaceae PTA-ELISA 


  1. Bezerra IC, Resende RO, Pozzer L, Nagata T, Kormelink R, Ávila AC (1999) Increase of tospoviral diversity in Brazil with the identification of two new tospovirus species, one from chrysanthemum and one from zucchini. Phytopathology 89:823–830CrossRefPubMedGoogle Scholar
  2. Giampan JS, Rezende JAM, Silva RF (2007) Reaction of cucurbits species to infection with Zucchini lethal chlorosis virus. Sci Hortic 114:129–132CrossRefGoogle Scholar
  3. Giampan JS, Rezende JAM, Piedade SMS (2009) Danos causados pelo Zucchini lethal chlorosis virus (ZLCV) sobre a produção de frutos comerciais de abobrinha de moita ‘Caserta’. Summa Phytopathol 35:223–225CrossRefGoogle Scholar
  4. Mound LA, Marullo R (1996) The thrips of Central and South America: an introduction. Mem Entomol Int 6:1–488Google Scholar
  5. Mowat WP, Dawson S (1987) Detection of plant viruses by ELISA using crude sap extracts unfractionated antisera. J Virol Methods 15:233–247CrossRefPubMedGoogle Scholar
  6. Nakahara S, Monteiro RC (1999) Frankliniella zucchini (Thysanoptera: Thripidae), a new species and vector of tospovirus in Brazil. Proc Entomol Soc Wash 101:290–294Google Scholar
  7. Pozzer L, Resende RO, Bezerra MI, Nagata T, Lima MI, Kitajima EW, Ávila AC (1996) Zucchini lethal chlorosis virus (ZLCV) a proposed new specie in Tospovirus genus. Fitopatol Bras 21(Suppl):432Google Scholar
  8. Resende RO, Pozzer L, Nagata T, Bezerra IC, Lima MI, Giordano LB, Kitajima EW, Ávila AC (1996) New tospovirus found in Brasil. Acta Horticult 41:79–89Google Scholar
  9. Rezende JAM, Galleti SR, Pozzer L, Resende RO, Ávila AC, Scagliusi SMM (1997) Incidence, biological, and serological characteristics of a tospovirus in experimental fields of zucchini in São Paulo State, Brazil. Fitopatol Bras 22:92–95Google Scholar
  10. Spadotti DMA, Buriolla JE, Rezende JAM, Souza VC (2013) The wild type of Momordica charantia is not infected by potyviruses that cause disease in papaya and cucurbit crops. Trop Plant Pathol 38:447–451CrossRefGoogle Scholar
  11. Toth IK, Hyman LJ, Bertheau Y, Fréchon D (2002) DNA amplification by polymerase chain reaction (PCR). In: Pérombelon MCM, Van Der Wolf JM (eds) Methods for the detection and quantification of Erwinia carotovora subsp. atroseptica (Pectobacterium carotovorum subsp. atrosepticum) on potatoes: a laboratory manual. Scottish Crop Research Institute Occasional Publication No. 10Google Scholar
  12. Yuki VA, Rezende JAM, Kitajima EW, Barroso PAV, Kuniyuki H, Groppo GA, Pavan MA (2000) Occurrence, distribution and relative incidence of viruses infecting cucurbits in the State of São Paulo, Brazil. Plant Dis 84:516–520CrossRefGoogle Scholar

Copyright information

© Sociedade Brasileira de Fitopatologia 2015

Authors and Affiliations

  • Viviana M. Camelo-García
    • 1
  • Élison F. B. Lima
    • 2
  • Jorge A. M. Rezende
    • 1
    Email author
  1. 1.Dep. de Fitopatologia e Nematologia, Escola Superior de Agricultura Luiz de QueirozUniversidade de São PauloPiracicabaBrazil
  2. 2.Universidade Federal do PiauíFlorianoBrazil

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