Tropical Plant Pathology

, Volume 40, Issue 1, pp 26–34 | Cite as

Novel Pathotypes of Elsinoë australis Associated with Citrus australasica and Simmondsia chinensis in Australia

  • Andrew K. MilesEmail author
  • Yu Pei Tan
  • Roger G. Shivas
  • André Drenth


Molecular phylogenetic analysis, morphology and pathogenicity to citrus fruit were used to study two isolates of Elsinoë australis associated with scab-like symptoms on a fruit of Citrus australasica (finger lime) and Simmondsia chinensis (jojoba) in Australia. In addition to being associated with finger lime, the isolate from finger lime could cause scab symptoms on C. × aurantium cv. Murcott tangor in pathogenicity tests, but could not cause scab symptoms on the other orange, mandarin, lemon or grapefruit tested. Pathogenicity tests also support previous studies showing the isolate from jojoba could not produce symptoms on fruit of C. natsudaidai. Based on the findings of this study, two novel pathotypes of E. australis are designated from Australia; namely the Finger Lime (FL) pathotype associated with finger lime, and the Jojoba Black Scab (JBS) pathotype associated with black scab of jojoba. The significance of these novel E. australis pathotypes on market access and biosecurity issues for citrus are briefly discussed.


ITS Phylogeny Scab Taxonomy TEF 



This research was partially funded by Horticulture Australia Limited using the citrus industry levy and matched funds from the Australian Government, and The University of Queensland, and the Queensland Department of Agriculture, Fisheries and Forestry. We wish to thank Dr. Mui Keng Tan and Dr. Nerida Donovan (NSW DPI) for providing the ‘jojoba black scab’ isolates. Dr. Jae-Wook Hyun (Rural Development Administration, National Institute of Horticultural & Herbal Science, Korea) for providing isolates of E. australis and E. fawcettii. Dr. Graeme Sanderson (NSW DPI) for providing the shoots of C. natsudaidai. Dr. Nerida Donovan (NSW DPI), Sylvia Jelinek (NSW DPI) and Tim Hermann (AusCitrus) for supplying various host plants. Dr. Jay Anderson (formerly DAFF Qld), Dr. Dean Beasley (DAFF Qld) and Cecilia O’Dwyer (UQ) for technical assistance, and Pat Barkley for advice on the manuscript.


  1. Ash GJ, Stodart B, Hyun JW (2012) Black scab of jojoba (Simmondsia chinensis) in Australia caused by a putative new pathotype of Elsinoë australis. Plant Dis 96:629–634CrossRefGoogle Scholar
  2. Bitancourt AA, Jenkins AE (1937) Sweet orange fruit scab caused by Elsinoë australis. J Agric Res 54:1–18Google Scholar
  3. Broadbent P (1995) Quarantine in relation to Australian citrus imports and exports. Australas Plant Pathol 24:145–156CrossRefGoogle Scholar
  4. Clarke K, Prakash N (2001) Floral morphology and embryology of two Australian species of Citrus (Rutaceae). Aust J Bot 49:199–207CrossRefGoogle Scholar
  5. Cunningham N (2002) Sooty mould cleaners and high pressure washing. Packer Newsl 67:1–3Google Scholar
  6. de Bary A (1974) Über den sogenannten Brenner der Reben. Ann der Oenologie 4:165–167Google Scholar
  7. Glienke C, Pereira OL, Stringari D, Fabris J, Kava-Cordeiro V, Galli-Terasawa L, Cunnington J, Shivas RG, Groenewald JZ, Crous PW (2011) Endophytic and pathogenic Phyllosticta species, with reference to those associated with Citrus Black Spot. Persoonia 26:47–56CrossRefPubMedCentralPubMedGoogle Scholar
  8. Hou X, Huang F, Zhang T, Xu J, Hyde DK, Ll H (2014) Pathotypes and genetic diversity of Chinese collections of Elsinoë fawcettii causing citrus scab. J Integr Agric 13:1293–1302Google Scholar
  9. Hyun JW, Timmer LW, Lee S-C, Yun S-H, Ko S-W, Kim KS (2001) Pathological characterization and molecular analysis of Elsinoë isolates causing scab diseases of citrus in Jeju Island in Korea. Plant Dis 85:1013–1017CrossRefGoogle Scholar
  10. Hyun JW, Yi SH, Mackenzie SJ, Timmer LW, Kim KS, Kang SK, Kwon HM, Lim HC (2009) Pathotypes and genetic relationship of worldwide collections of Elsinoë spp. causing scab diseases of citrus. Phytopathology 99:721–728CrossRefPubMedGoogle Scholar
  11. Jenkins AE, Bitancourt AA (1941) Revised descriptions of the genera Elsinoë and Sphaceloma. Mycologia 33:338–340CrossRefGoogle Scholar
  12. Kirk PM, Cannon PF, Minter DW, Stalper JA (2008) Dictionary of the Fungi, 10th edn. CABI International, SurreyGoogle Scholar
  13. Kunta M, Rascoe J, de Sa P, Timmer LW, Palm ME, da Graca JV, Mangan RL, Mangan RL, Malik N, Salas B, Satpute A, Setamou M, Skaria M (2013) Sweet orange scab with a new scab disease “syndrome” of citrus in the USA associated with Elsinoë australis. Trop Plant Pathol 38:203–212CrossRefGoogle Scholar
  14. Raciborski M (1900) Parasitische Algen und Pilze Java’s. Batavia, StaatsdruckereiGoogle Scholar
  15. Rayner RW (1970) A mycological colour chart. Kew, England. Commonwealth Mycological InstituteGoogle Scholar
  16. Ronquist FJ, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574CrossRefPubMedGoogle Scholar
  17. Saccardo PA, Sydow P (1902) Sylloge Fungorum omnium hucusque cognitorum. Digessit P.A. Saccardo 16:804Google Scholar
  18. Stamatakis A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690CrossRefPubMedGoogle Scholar
  19. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefPubMedCentralPubMedGoogle Scholar
  20. Tan MK, Timmer LW, Broadbent P, Priest M, Cain P (1996) Differentiation by molecular analysis of Elsinoë spp. causing scab diseases of citrus and its epidemiological implications. Phytopathology 86:1039–1044CrossRefGoogle Scholar
  21. Taverner P (2012) Sodium o-phenylphenate tetrahydrate (SOPP). Packer Newsl 103:3–5Google Scholar
  22. Taverner P, Cunningham N (1999) Sanitation survey of Riverland packingsheds. Packer Newsl 56:1–5Google Scholar
  23. Timmer LW (2000) Scab diseases. In: Timmer LW, Garnsey SM, Graham JH (eds) Compendium of citrus diseases. APS Press, St Paul, pp 31–32Google Scholar
  24. Timmer LW, Priest M, Broadbent P, Tan MK (1996) Morphological and pathological characterization of species of Elsinoë causing scab diseases of citrus. Phytopathology 86:1032–1038CrossRefGoogle Scholar
  25. USDA APHIS PPQ (2010) The significance of Citrus spp. fruit as a pathway for the introduction or spread of Elsinoë australis, the organism that causes Sweet orange scab disease. Raleigh, NC, USA. Center for Plant Health Science and Technology, Plant Epidemiology and Risk Analysis LaboratoryGoogle Scholar
  26. Wang L, Liao H, Bau H, Chung K (2009) Characterization of pathogenic variants of Elsinoë fawcettii of citrus implies the presence of new pathotypes and cryptic species in Florida. Can J Plant Pathol 31:28–37CrossRefGoogle Scholar
  27. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols a guide to methods and applications. USA. Academic Press, San Diego, pp 315–322CrossRefGoogle Scholar
  28. Whiteside JO (1975) Biological characteristics of Elsinoë fawcettii pertaining to the epidemiology of sour orange scab. Phytopathology 65:1170–1175CrossRefGoogle Scholar
  29. Winston JR (1923) Citrus scab: its cause and control. Bulletin 118. Beltsville, MD, USA. United States Department of AgricultureGoogle Scholar

Copyright information

© Sociedade Brasileira de Fitopatologia 2015

Authors and Affiliations

  • Andrew K. Miles
    • 1
    • 4
    Email author
  • Yu Pei Tan
    • 2
  • Roger G. Shivas
    • 2
  • André Drenth
    • 3
  1. 1.Formerly Department of Agriculture Fisheries and ForestryAgri-Science QueenslandDutton ParkAustralia
  2. 2.Department of Agriculture Fisheries and ForestryPlant Pathology Herbarium, Biosecurity QueenslandDutton ParkAustralia
  3. 3.The University of Queensland, Centre for Plant ScienceDutton ParkAustralia
  4. 4.Research and Development for Primary Industries Pty LtdIndooroopillyAustralia

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