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Thrombosis Model in Mouse Carotid Induced by Guidewire

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Abstract

Researchers have developed many thrombosis models in the mouse. Some methods for establishing the model require specialized equipment and have high cost. The present study develops a low-cost, easy-to-operate, and well-controlled vascular injury thrombosis model in the mouse carotid artery induced by a guidewire. Superficial injury was induced in the carotid artery by scratching with a guidewire. Thrombus formation was observed and recorded in real time. Thrombosis quantification analyses were performed using Metamorph and Graphpad software. Transmission electron microscopy (TEM), scanning electron microscopy (SEM), and hematoxylin and eosin (HE) staining were employed for the histological analysis of the injured vessel wall. Three clinical drugs were respectively administrated to this model for further evaluation. Complete removal of the endothelium was observed by TEM in this injury model. Platelets covered the denuded surface, in which the aggregates were detected by HE staining and SEM. A transient thrombus reaching peak at around 1 min after injury and vanishing gradually within 3 min formed in the model. Administration of clinical medicaments decreased thrombus formation in this model. All lesions were sensitive to platelet GPIIb-IIIa, P2Y12 inhibition, and thrombin blockade. The proposed model has good reproducibility and feasibility and could be applied in therapeutic targeting and anti-thrombotic drug screening.

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Acknowledgments

This study was funded by NNSF of China (Grant No. 31300781) and the Fundamental Research Funds for the Central Universities (Project No. CQDXWL-2012-125). We thank Dr. Christian Gachet’s laboratory and Dr. Pierre H. Mangin’s laboratory in EFS-Alsace, France, for kindly providing partial experimental support. Partial work was supported by FFCSA (Fondation Franco-Chinoise pour la Science et ses Applications), the National “111 plan” Base of China (B06023) as well as Public Experiment Center of State Bioindustrial Base (Chongqing) of China.

Author information

Correspondence to Chaojun Tang or Guixue Wang.

Additional information

Lu Huang and Daoxi Lei have contributed equally to this work.

Electronic supplementary material

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The movie displayed the developing thrombi post-injury with guidewire scratching in the carotid. 20 seconds in movie represented 5 minutes in real-time (WMV 1226 kb)

The movie displayed the developing thrombi post-injury with guidewire scratching in the carotid. 20 seconds in movie represented 5 minutes in real-time (WMV 1226 kb)

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Huang, L., Lei, D., Dong, W. et al. Thrombosis Model in Mouse Carotid Induced by Guidewire. J. Med. Biol. Eng. 36, 236–244 (2016) doi:10.1007/s40846-016-0125-0

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Keywords

  • Animal model
  • Vascular injury
  • Thrombosis
  • Anti-thrombotic
  • Guidewire