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Gold immunochromatographic assay for simultaneous detection of sibutramine and sildenafil in slimming tea and coffee

胶体金免疫层析分析方法用于同时检出减肥茶和咖啡中西布曲明和西地那非

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摘要

本文建立了一种方便、快速、高特异性的胶体金免疫层析检测方法, 用于同时检测减肥茶和咖啡中的西布曲明(SB)和西地那非(SID). 我们首先制备了胶体金纳米粒子(CG), 然后使之与单抗结合形成CG-mAb, 将CG-mAb与抗原分别喷涂到基板上形成结合垫和检测条带, 其中所需的抗原和抗体均由本实验室制备. 经过条件优化, 该方法在PBS缓冲溶液(0.01 mol L−1, pH 7.4), 以及三种减肥茶和三种咖啡样本中, 对SB和SID的消线值均为500 ng mL−1; 检测时间仅需5 min. 该方法对减肥茶和咖啡样本中SB和SID的快速检出具有实际应用价值.

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Acknowledgements

This work was financially supported by the National Key R&D Program of China (2016YFD0401101 and KYCX18_1566).

Author information

Xu C and Kuang H designed the experiments. Suryoprabowo S and Liu L performed the experiments. Cui G and Kuang H participated in data analysis. Xu C drafted the manuscript, and all authors read and approved the manuscript prior to submission.

Correspondence to Chuanlai Xu 胥传来.

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Conflict of interest

The authors declare that they have no conflict of interest.

Steven Suryoprabowo is a PhD candidate under the supervision of Prof. Chuanlai Xu at Jiangnan University. His research interests focus on developing rapid and sensitive methods to determine analytes using immunochromatography strip test.

Chuanlai Xu is the Director of International Lab of Biointerface & Biodetection, Jiangnan University. Leader of the Food Safety Innovation Team in Priority Areas Accredited by the Ministry of Science and Technology, Fellow of the Royal Society of Chemistry. He worked as a visiting scholar at the University of East Anglia, UK in 2007 and University of Michigan in 2008, respectively. His research interests include: (1) self-assembly of advanced inorganic materials and optical properties discovery; (2) fabrication of nano-devices and their applications in biotechnology. He combines nanotechnology and biological recognition to promote the real application of novel biosensors. He has been authorized more than 210 Chinese or international patents and realized commercialization of more than 500 monoclonal antibodies. He has published more than 350 scientific papers, 3 invited book chapters and 3 books. He was awarded four times for the 2nd National Science and Technology Progress Award (2007, 2009, 2011, and 2017) and Special Allowance of Chinese State Council. He serves as an Editor in Chief, Food Agricultural Immunology, and Editorial Boards of Science China Materials, Critical Reviews in Analytical Chemistry, Current Nanoscience, etc.

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Suryoprabowo, S., Liu, L., Kuang, H. et al. Gold immunochromatographic assay for simultaneous detection of sibutramine and sildenafil in slimming tea and coffee. Sci. China Mater. (2020) doi:10.1007/s40843-019-1228-2

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