Gold immunochromatographic assay for simultaneous detection of sibutramine and sildenafil in slimming tea and coffee


  • 35 Accesses


本文建立了一种方便、快速、高特异性的胶体金免疫层析检测方法, 用于同时检测减肥茶和咖啡中的西布曲明(SB)和西地那非(SID). 我们首先制备了胶体金纳米粒子(CG), 然后使之与单抗结合形成CG-mAb, 将CG-mAb与抗原分别喷涂到基板上形成结合垫和检测条带, 其中所需的抗原和抗体均由本实验室制备. 经过条件优化, 该方法在PBS缓冲溶液(0.01 mol L−1, pH 7.4), 以及三种减肥茶和三种咖啡样本中, 对SB和SID的消线值均为500 ng mL−1; 检测时间仅需5 min. 该方法对减肥茶和咖啡样本中SB和SID的快速检出具有实际应用价值.


  1. 1

    Yuan R. Traditional Chinese medicine an approach to scientific proof and clinical validation. Pharmacol Therap, 2000, 86: 191–198

  2. 2

    Yun J, Shin KJ, Choi J, et al. Isolation and structural characterization of a novel sibutramine analogue, chlorosipentramine, in a slimming dietary supplement, by using HPLC-PDA, LC-Q-TOF/MS, FT-IR, and NMR. Forensic Sci Int, 2018, 286: 199–207

  3. 3

    Phattanawasin P, Sotanaphun U, Sukwattanasinit T, et al. Quantitative determination of sibutramine in adulterated herbal slimming formulations by TLC-image analysis method. Forensic Sci Int, 2012, 219: 96–100

  4. 4

    de Carvalho LM, Martini M, Moreira APL, et al. Presence of synthetic pharmaceuticals as adulterants in slimming phytotherapeutic formulations and their analytical determination. Forensic Sci Int, 2011, 204: 6–12

  5. 5

    Müller D, Weinmann W, Hermanns-Clausen M. Chinese slimming capsules containing sibutramine sold over the internet: A case series. Deutsches Äerzteblatt International, 2009, 106: 218–222

  6. 6

    Wang S, Yu J, Wan F, et al. Determination of sibutramine with a new sensor based on luminol electrochemiluminescence. J Lumin, 2011, 131: 1515–1519

  7. 7

    Terrett NK, Bell AS, Brown D, et al. Sildenafil (VIAGRATM), a potent and selective inhibitor of type 5 cGMP phosphodiesterase with utility for the treatment of male erectile dysfunction. Bioorg Med Chem Lett, 1996, 6: 1819–1824

  8. 8

    Ortiz RS, Mariotti KC, Schwab NV, et al. Fingerprinting of sildenafil citrate and tadalafil tablets in pharmaceutical formulations via X-ray fluorescence (XRF) spectrometry. J Pharm Biomed Anal, 2012, 58: 7–11

  9. 9

    Wollein U, Schech B, Hardt J, et al. Determination and quantitation of sildenafil and its major metabolite in the breast milk of a lactating woman. J Pharm Biomed Anal, 2016, 120: 100–105

  10. 10

    Man CN, Nor NM, Lajis R, et al. Identification of sildenafil, tadalafil and vardenafil by gas chromatography-mass spectrometry on short capillary column. J Chromatog A, 2011, 1218: 4788

  11. 11

    Li Y, Hu J, Shi Y, et al. Simultaneous determination of seven adulterants in slimming functional foods by HPLC-ESI-MS/MS. Food Anal Methods, 2011, 4: 505–516

  12. 12

    Strano-Rossi S, Colamonici C, Botrè F. Detection of sibutramine administration: A gas chromatography/mass spectrometry study of the main urinary metabolites. Rapid Commun Mass Spectrom, 2007, 21: 79–88

  13. 13

    Yamamoto S, Sumioka S, Fujioka M, et al. A study on detection of drugs in slimming health foods using GC-MS/MS. J Food Hyg Soc Jpn, 2011, 52: 363–369

  14. 14

    Strano-Rossi S, Anzillotti L, de la Torre X, et al. A gas chromatography/mass spectrometry method for the determination of sildenafil, vardenafil and tadalafil and their metabolites in human urine. Rapid Commun Mass Spectrom, 2010, 24: 1697–1706

  15. 15

    Jeong Y, Suh SI, Kim JY, et al. Simultaneous determination of sildenafil, tadalafil, and vardenafil in pharmaceutical preparations by high-temperature gas chromatography/mass spectrometry. Chromatographia, 2016, 79: 1671–1678

  16. 16

    Wang K, Zeng H, Zhang Y, et al. A hierarchical screening method for detection of illegal adulterants in Fur seal ginseng pills by profiling analysis of HPLC multi-dimensional fingerprints. J Sep Sci, 2019, 42: 1509–1519

  17. 17

    Mathon C, Ankli A, Reich E, et al. Screening and determination of sibutramine in adulterated herbal slimming supplements by HPTLC-UV densitometry. Food Add Contams-Part A, 2014, 31: 15–20

  18. 18

    Hemdan A, Tawakol SM. HPLC-UV chromatographic methods for detection and quantification of undeclared withdrawn synthetic medications in counterfeit herbal medicines with confirmation by HPLC-PDA and mass spectrometry. Chromatographia, 2018, 81: 777–783

  19. 19

    Zeng Y, Xu Y, Kee CL, et al. Analysis of 40 weight loss compounds adulterated in health supplements by liquid chromatography quadrupole linear ion trap mass spectrometry. Drug Test Anal, 2016, 8: 351–356

  20. 20

    Zhong Y, Sun C, Xiong J, et al. Simultaneous determination of eight adulterants in weight management supplements and herbs by HPLC-DAD and LC-MS/MS. J Liquid Chromatog Related Technol, 2017, 40: 640–648

  21. 21

    Rashid J, Ahsan F. A highly sensitive LC-MS/MS method for concurrent determination of sildenafil and rosiglitazone in rat plasma. J Pharm Biomed Anal, 2016, 129: 21–27

  22. 22

    Hasnain MS, Ansari SA, Rao S, et al. QbD-driven development and validation of liquid chromatography tandem mass spectrometric method for the quantitation of sildenafil in human plasma. J Chromatog Sci, 2017, 55: 587–594

  23. 23

    Ge W, Suryoprabowo S, Xu L, et al. Rapid detection of penbutolol in pig urine using an immunochromatographic test strip. Food Agric Immunol, 2018, 29: 1126–1136

  24. 24

    Hao K, Suryoprabowo S, Song S, et al. Rapid detection of zear-alenone and its metabolite in corn flour with the immunochromatographic test strip. Food Agric Immunol, 2018, 29: 498–510

  25. 25

    Liu L, Xu L, Suryoprabowo S, et al. Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine. Food Agric Immunol, 2018, 29: 434–444

  26. 26

    Song S, Suryoprabowo S, Liu L, et al. Development of monoclonal antibody-based colloidal gold immunochromatographic assay for analysis of halofuginone in milk. Food Agric Immunol, 2019, 30: 112–122

  27. 27

    Song Y, Wang YY, Zhang Y, et al. Development of enzyme-linked immunosorbent assay for rapid determination of sildenafil in adulterated functional foods. Food Agric Immunol, 2012, 23: 338–351

  28. 28

    Shen X, Liu L, Xu L, et al. Rapid detection of praziquantel using monoclonal antibody-based ic-ELISA and immunochromatographic strips. Food Agric Immunol, 2019, 30: 913–923

  29. 29

    Kong D, Liu L, Song S, et al. A gold nanoparticle-based semi-quantitative and quantitative ultrasensitive paper sensor for the detection of twenty mycotoxins. Nanoscale, 2016, 8: 5245–5253

  30. 30

    Isanga J, Mukunzi D, Chen Y, et al. Development of a monoclonal antibody assay and a lateral flow strip test for the detection of paromomycin residues in food matrices. Food Agric Immunol, 2017, 28: 355–373

  31. 31

    Gong X, Liu Y, Xiang H, et al. Membraneless reproducible MoS2 field-effect transistor biosensor for high sensitive and selective detection of FGF21. Sci China Mater, 2019, 62: 1479–1487

  32. 32

    Jiang W, Zeng L, Liu L, et al. Immunochromatographic strip for rapid detection of phenylethanolamine A. Food Agric Immunol, 2018, 29: 182–192

  33. 33

    Xu Y, Wang H, Chen B, et al. Emerging barcode particles for multiplex bioassays. Sci China Mater, 2019, 62: 289–324

  34. 34

    Huang P, Tu D, Zheng W, et al. Inorganic lanthanide nanoprobes for background-free luminescent bioassays. Sci China Mater, 2015, 58: 156–177

  35. 35

    Wang W, Liu L, Song S, et al. Gold nanoparticle-based strip sensor for multiple detection of twelve Salmonella strains with a genus-specific lipopolysaccharide antibody. Sci China Mater, 2016, 59: 665–674

  36. 36

    Guo L, Xu L, Song S, et al. Development of an immunochromatographic strip for the rapid detection of maduramicin in chicken and egg samples. Food Agric Immunol, 2018, 29: 458–469

  37. 37

    Guo J, Liu W, Lan X, et al. Development and evaluation of an immunochromatographic strip for rapid screening of sildenafil-type compounds as illegal additives in functional foods. Food Additives Contams-Part A, 2016, 33: 1095–1104

Download references


This work was financially supported by the National Key R&D Program of China (2016YFD0401101 and KYCX18_1566).

Author information

Xu C and Kuang H designed the experiments. Suryoprabowo S and Liu L performed the experiments. Cui G and Kuang H participated in data analysis. Xu C drafted the manuscript, and all authors read and approved the manuscript prior to submission.

Correspondence to Chuanlai Xu 胥传来.

Additional information

Conflict of interest

The authors declare that they have no conflict of interest.

Steven Suryoprabowo is a PhD candidate under the supervision of Prof. Chuanlai Xu at Jiangnan University. His research interests focus on developing rapid and sensitive methods to determine analytes using immunochromatography strip test.

Chuanlai Xu is the Director of International Lab of Biointerface & Biodetection, Jiangnan University. Leader of the Food Safety Innovation Team in Priority Areas Accredited by the Ministry of Science and Technology, Fellow of the Royal Society of Chemistry. He worked as a visiting scholar at the University of East Anglia, UK in 2007 and University of Michigan in 2008, respectively. His research interests include: (1) self-assembly of advanced inorganic materials and optical properties discovery; (2) fabrication of nano-devices and their applications in biotechnology. He combines nanotechnology and biological recognition to promote the real application of novel biosensors. He has been authorized more than 210 Chinese or international patents and realized commercialization of more than 500 monoclonal antibodies. He has published more than 350 scientific papers, 3 invited book chapters and 3 books. He was awarded four times for the 2nd National Science and Technology Progress Award (2007, 2009, 2011, and 2017) and Special Allowance of Chinese State Council. He serves as an Editor in Chief, Food Agricultural Immunology, and Editorial Boards of Science China Materials, Critical Reviews in Analytical Chemistry, Current Nanoscience, etc.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Suryoprabowo, S., Liu, L., Kuang, H. et al. Gold immunochromatographic assay for simultaneous detection of sibutramine and sildenafil in slimming tea and coffee. Sci. China Mater. (2020) doi:10.1007/s40843-019-1228-2

Download citation