Abstract
Plasmopara viticola, a causal agent of grapevine downy mildew, is a widely distributed pathogen, which can cause destructive disease in field-grown grapevines. Although fungicides are used to treat the disease, fungicide-resistant strains have been emerging. In this study, we developed graphene oxide (GO)-Fe3O4 nanocomposites, which could effectively repress the germination of sporangia and inhibit the development of downy mildew. 50 μg mL−1 GO-Fe3O4 showed excellent protective and fungicidal activities. 250 μg mL−1 GO-Fe3O4 on grapevine leaves in the field could significantly decrease the severity of downy mildew, suggesting its potent curative effect. Moreover, GO-Fe3O4 had no significant toxic effects on grapevine plants even at the concentration twice that of the highest dosage (1000 μg mL−1) used in this study. Our work suggested that GO-Fe3O4 would offer an important opportunity to develop new approach for controlling plant diseases.
摘要
葡萄霜霉病由葡萄霜霉菌引起, 是一种广泛存在的葡萄病害, 严重威胁葡萄的田间正常生长. 虽然化学杀菌剂能有效控制病害, 但是 容易导致病菌产生抗药性. 本文研究发现氧化石墨烯-四氧化三铁复合纳米材料可以有效地抑制葡萄霜霉菌的孢子萌发并控制霜霉病的 发展. 试验结果表明, 50 μg mL−1的氧化石墨烯-四氧化三铁复合纳米材料对葡萄霜霉病具有保护作用和杀菌效果. 田间试验结果表明, 250 μg mL−1氧化石墨烯-四氧化三铁复合纳米材料可以显著降低葡萄霜霉病的病情指数, 具有较好的治疗作用. 高剂量(1000 μg mL−1)的氧化石 墨烯-四氧化三铁复合纳米材料对葡萄叶片没有表现出明显的毒性. 本研究结果表明氧化石墨烯-四氧化三铁复合纳米材料有望成为防治 葡萄霜霉病的一种新方法.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (31501680 and 21277055), the Natural Science Foundation of Hebei Province (C2014407061 and C2014407008), and PhD Research Startup Foundation ofHebei Normal University of Science and Technology (2013YB005).
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Xiuping Wang received her PhD degree in pesticide science from Huazhong Agriculture University in 2012. Now she is a lecturer at the College of Life Science and Technology, Hebei Normal University of Science and Technology. Her current research interests are in the intersection between plant protection and material science.
Hong Yuan received his PhD degree in analytical chemistry from the College of Chemistry and Molecular Sciences,Wuhan University, Wuhan, China, in 2003. Currently, he is a professor at the College of Chemistry, Central China Normal University. His research interests include the application of nanomaterials in environmental chemistry.
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Wang, X., Cai, A., Wen, X. et al. Graphene oxide-Fe3O4 nanocomposites as high-performance antifungal agents against Plasmopara viticola . Sci. China Mater. 60, 258–268 (2017). https://doi.org/10.1007/s40843-016-9005-9
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DOI: https://doi.org/10.1007/s40843-016-9005-9