Comparison of molecular and extract-based allergy diagnostics with multiplex and singleplex analysis
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ImmunoCAP ISAC 112, is a commercially available molecular allergy IgE multiplex test. Data on the comparison of this rather novel test with extract-based as well as molecular ImmunoCAP singleplex IgE tests is missing.
To perform a comparison between the ISAC multiplex IgE assay and the ImmunoCAP singleplex test results.
Serum samples of 101 adults with grass pollen allergy were analysed for sIgE to 112 allergenic molecules represented on the ISAC test as well as to common atopy-related extract-based allergy tests with the ImmunoCAP System (house dust mite [d1], cat [e1], dog [e5], cow’s milk [f2], hen’s egg [f1], hazelnut [f17], celery [f85], Alternaria alternate [m6], as well as pollen from birch [t3], hazel [t4], mugwort [w6], and ragweed [w1]). Subsequently statistical analysis was performed with the Spearman rank correlation test and the Clopper-Pearson method in order to compare the ISAC multiplex results with the sIgE singleplex results.
The positive percent agreements (PPA) and negative percent agreement (NPA) of corresponding allergens between the ISAC sIgE test and the extract-based singleplex ImmunoCAP results at cutoff 0.1 kUA/l varied between 60–100 % for PPA and 78–97 % for NPA.
When taking into account corresponding allergens molecular testing with the ISAC multiplex test correlates well with ImmunoCAP singleplex results.
KeywordsMolecular allergy diagnostics extract-based diagnostics singleplex multiplex Immuno-CAP ISAC component resolved diagnosis allergens specific IgE
Cross-reactive carbohydrate determinant
Coefficient of variation
Immuno solid-phase allergen chip
ISAC standardized unit
ImmunoCAP specific IgE
Kilounits (corresponding allergens)
Limit of detection
Molecular allergy diagnostics
Negative percent agreement
Positive percent agreement
Specific Immunglobulin E
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