Allergo Journal International

, Volume 24, Issue 2, pp 46–53 | Cite as

Comparison of molecular and extract-based allergy diagnostics with multiplex and singleplex analysis

  • Johannes Huss-MarpEmail author
  • Jan Gutermuth
  • Ina Schäffner
  • Ulf Darsow
  • Florian Pfab
  • Knut Brockow
  • Johannes Ring
  • Heidrun Behrendt
  • Thilo Jakob
  • Christoph Ahlgrim
Original article



ImmunoCAP ISAC 112, is a commercially available molecular allergy IgE multiplex test. Data on the comparison of this rather novel test with extract-based as well as molecular ImmunoCAP singleplex IgE tests is missing.


To perform a comparison between the ISAC multiplex IgE assay and the ImmunoCAP singleplex test results.


Serum samples of 101 adults with grass pollen allergy were analysed for sIgE to 112 allergenic molecules represented on the ISAC test as well as to common atopy-related extract-based allergy tests with the ImmunoCAP System (house dust mite [d1], cat [e1], dog [e5], cow’s milk [f2], hen’s egg [f1], hazelnut [f17], celery [f85], Alternaria alternate [m6], as well as pollen from birch [t3], hazel [t4], mugwort [w6], and ragweed [w1]). Subsequently statistical analysis was performed with the Spearman rank correlation test and the Clopper-Pearson method in order to compare the ISAC multiplex results with the sIgE singleplex results.


The positive percent agreements (PPA) and negative percent agreement (NPA) of corresponding allergens between the ISAC sIgE test and the extract-based singleplex ImmunoCAP results at cutoff 0.1 kUA/l varied between 60–100 % for PPA and 78–97 % for NPA.


When taking into account corresponding allergens molecular testing with the ISAC multiplex test correlates well with ImmunoCAP singleplex results.


Molecular allergy diagnostics extract-based diagnostics singleplex multiplex Immuno-CAP ISAC component resolved diagnosis allergens specific IgE 



Cross-reactive carbohydrate determinant


Confidence interval


Coefficient of variation


Immunglobulin E


Immuno solid-phase allergen chip


ISAC standardized unit


ImmunoCAP specific IgE


Kilounits (corresponding allergens)


Limit of detection


Molecular allergy diagnostics


Negative percent agreement


Positive percent agreement


Standard deviation


Specific Immunglobulin E


Specific Immunotherapy


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Copyright information

© Urban & Vogel 2015

Authors and Affiliations

  • Johannes Huss-Marp
    • 1
    • 2
    • 9
    Email author
  • Jan Gutermuth
    • 3
  • Ina Schäffner
    • 2
  • Ulf Darsow
    • 4
    • 5
  • Florian Pfab
    • 4
    • 5
  • Knut Brockow
    • 4
    • 5
  • Johannes Ring
    • 4
  • Heidrun Behrendt
    • 5
  • Thilo Jakob
    • 1
  • Christoph Ahlgrim
    • 6
    • 7
    • 8
  1. 1.Allergy Research Group, Department of DermatologyMedical Center, University of FreiburgFreiburgGermany
  2. 2.Thermo Fisher ScientificPhadia GmbHFreiburgGermany
  3. 3.Department of Dermatology, Universitair Ziekenhuis BrusselVrije Universiteit Brussel (VUB)BrusselsBelgium
  4. 4.Department of Dermatology and AllergyTechnische Universität MünchenMunichGermany
  5. 5.ZAUM-Center for Allergy and Environment, Helmholtz Center MunichTechnische Universität MünchenMunichGermany
  6. 6.University Freiburg Medical Center, Department of Exercise Medicine and Sport, University of FreiburgFreiburgGermany
  7. 7.Department of PneumologyUniversity of FreiburgFreiburgGermany
  8. 8.Department of PneumologyFreiburg University Medical Center, Albert-Ludwigs-UniversityFreiburgGermany
  9. 9.AbbVie Deutschland GmbH & Co. KGWiesbadenGermany

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