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DIMBOA levels in hexaploid Brazilian wheat are not associated with antibiosis against the cereal aphids Rhopalosiphum padi and Sitobion avenae

  • Jorge F. PereiraEmail author
  • André L. F. Sarria
  • Stephen J. Powers
  • Gudbjorg I. Aradottir
  • John C. Caulfield
  • Janet Martin
  • Lesley E. Smart
  • John A. Pickett
  • Michael A. Birkett
  • Paulo R. V. S. Pereira
Article

Abstract

The objective of this study was to evaluate the natural levels of the plant defence compound DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one) in young leaves of eight hexaploid Brazilian wheat genotypes and the impact of the genotypes upon development of cereal aphids, Rhopalosiphum padi and Sitobion avenae. HPLC analysis revealed that the DIMBOA levels varied from 5.376 (in BRS Guabiju) to 30.651 mmol kg FW−1 (in BRS Timbaúva) with two genotypes outperforming Solstice, a UK variety used as reference. Bioassays were conducted to evaluate the development and fecundity of both aphids when grown on the wheat genotypes. Although BRS Guabiju and BRS Timbaúva were among the genotypes showing the highest susceptibility and resistance, respectively, against both aphid species, no correlation could be found between DIMBOA levels and antibiosis effects. The cultivar BRS 327 was among the genotypes showing lower intrinsic rate of population increase for the two aphid species. Elucidating the role of secondary metabolites in plant resistance to aphids and the characterization of the genotypes that allowed reduced development of R. padi and S. avenae are important steps to achieve a better natural resistance in hexaploid Brazilian wheat.

Keywords

Aphid development HPLC Hydroxamic acids Plant resistance Triticum aestivum 

Notes

Acknowledgements

We are thankful for the financial support of Rothamsted International Fellowship Scheme, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)—process number 211732/2013-3, Embrapa (Empresa Brasileira de Pesquisa Agropecuária) and Biotechnology and Biological Sciences Research Council (BBSRC) of the United Kingdom (BB/L02652X/1). We also thank Dr Keith Chamberlain from Rothamsted Research for helping with the HPLC analysis and Dr Douglas Lau from Embrapa Trigo for helpful discussions.

Supplementary material

40626_2017_84_MOESM1_ESM.docx (1.3 mb)
Supplementary material 1 (DOCX 1328 kb)

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Copyright information

© Brazilian Society of Plant Physiology 2017

Authors and Affiliations

  • Jorge F. Pereira
    • 1
    • 2
    Email author
  • André L. F. Sarria
    • 3
  • Stephen J. Powers
    • 4
  • Gudbjorg I. Aradottir
    • 3
  • John C. Caulfield
    • 3
  • Janet Martin
    • 3
  • Lesley E. Smart
    • 3
  • John A. Pickett
    • 3
  • Michael A. Birkett
    • 3
  • Paulo R. V. S. Pereira
    • 1
  1. 1.Embrapa TrigoPasso FundoBrazil
  2. 2.Embrapa Gado de LeiteJuiz de ForaBrazil
  3. 3.Biological Chemistry and Crop Protection DepartmentRothamsted ResearchHertfordshireUK
  4. 4.Computational and Systems Biology DepartmentRothamsted ResearchHertfordshireUK

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