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Theoretical and Experimental Plant Physiology

, Volume 26, Issue 3–4, pp 167–175 | Cite as

Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress

  • Alana Cecília Menezes Sobreira
  • Yuri Maia
  • Deborah Moura Rebouças
  • Nila Maria Bezerril Fontenele
  • José Hélio Costa
  • Maria de Lourdes Oliveira Otoch
  • Luciana Maia Nogueira de Oliveira
  • Elena Graciela Orellano
  • Dirce Fernandes de MeloEmail author
Article

Abstract

Global climatic changes as high temperatures and low precipitation contribute to increase cultivated areas affected by high salt soil content. Soil salinity is well known to reduce the ability of plants to take up water and this quickly causes reduction in their growth rate. V-ATPase (EC 3.6.3.14) and V-PPase (EC 3.6.1.1) hydrolytic and proton transport activities, and gene expression were evaluated in hypocotyls of 3-, 5-, 7-day-old Vigna unguiculata (L.) Walp cv. Vita 3 germinated in 100 mM NaCl in order to highlight their differential regulation and activity modulation under salt stress. Semi-quantitative RT-PCR revealed that both genes were up-regulated by salt stress in all salt exposition times studied. Up-regulation was correlated with the increase in protein content at 5 and 7-day-old seedlings. Co-expression between A and E V-ATPase subunits was also observed. The hydrolytic and proton transport activities showed that these enzymes presented a differential modulation of their activities in the presence of 100 mM NaCl. These results suggest that V-ATPase and V-PPase activities are modulated by salt stress and a multi-step regulation is exerted in order to re-establish homeostasis.

Keywords

Cowpea Proton pump Salinity stress Vacuolar membrane 

Abbreviations

ACMA

9-Amino-6-chloro-2-methoxyacridine

BTP

Bis-tris-propane [1,3-bis(tris(hydroxyl-methyl)methylamino)-propane]

DTT

Dithiothreitol

EDTA

Ethylene diamine tetra-acetic acid

PMSF

Phenylmethyl-sulfonyl fluoride

V-ATPase

Vacuolar H+-ATPase (EC 3.6.3.14)

V-PPase

Vacuolar H+-PPase (EC 3.6.1.1)

Notes

Acknowledgments

We thank Dr M. Maeshima for the kind gift of the antibody against V-ATPase subunit A and V-PPase and Dr Y. Kawamura for the kind gift of the antibody against V-ATPase subunit E.

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Copyright information

© Brazilian Society of Plant Physiology 2014

Authors and Affiliations

  • Alana Cecília Menezes Sobreira
    • 1
    • 2
  • Yuri Maia
    • 1
  • Deborah Moura Rebouças
    • 1
  • Nila Maria Bezerril Fontenele
    • 1
  • José Hélio Costa
    • 1
  • Maria de Lourdes Oliveira Otoch
    • 3
  • Luciana Maia Nogueira de Oliveira
    • 4
  • Elena Graciela Orellano
    • 5
    • 6
  • Dirce Fernandes de Melo
    • 1
    Email author
  1. 1.Laboratório de Bioenergética, Departamento de Bioquímica e Biologia MolecularUniversidade Federal do CearáFortalezaBrazil
  2. 2.Faculdade de Educação, Ciências e Letras de Iguatu – FECLIUniversidade Estadual do CearáIguatuBrazil
  3. 3.Centro de Ciências da SaúdeUniversidade Estadual do CearáCampus Itaperi FortalezaBrazil
  4. 4.Unidade Acadêmica de GaranhunsUniversidade Federal Rural de PernambucoPEBrazil
  5. 5.Molecular Biology Division Instituto de Biología Molecular y Celular de RosarioConsejo Nacional de Investigaciones Científicas y TécnicasRosarioArgentina
  6. 6.Facultad de Ciencias Bioquímicas y FarmacéuticasUniversidad Nacional de RosarioRosarioArgentina

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