Journal of Endocrinological Investigation

, Volume 42, Issue 5, pp 549–555 | Cite as

Effects of metabolic abnormalities, hyperandrogenemia and clomiphene on liver function parameters among Chinese women with polycystic ovary syndrome: results from a randomized controlled trial

  • W.-Y. Cai
  • J.-S. Gao
  • X. Luo
  • H.-L. Ma
  • H. Ge
  • N. Liu
  • Q. Xia
  • Y. Wang
  • B.-W. Han
  • X.-K. WuEmail author
Original Article



To investigate the effects of metabolic abnormalities, hyperandrogenemia and ovulation induction by clomiphene/acupuncture on liver function parameters among women with polycystic ovary syndrome (PCOS).


This is a secondary analysis of a randomized controlled trial. All 1000 subjects were diagnosed as PCOS by modified Rotterdam criteria. Liver function parameters, metabolic panel and hormone profile were measured at baseline and after treatment. The relationship between liver parameters with metabolic, hormonal parameters and ovulation induction was examined.


PCOS women with metabolic syndrome had higher liver enzyme levels but lower bilirubin and bile acid levels than without. PCOS women with hyperandrogenemia had higher liver enzyme, bilirubin levels than without. Correlation analyses showed that worsening of metabolic parameters was associated with higher liver enzyme levels but lower bilirubin and bile acid levels, while increased androgen levels were associated with higher liver enzyme, bilirubin and bile acid levels. Ovulation induction with clomiphene citrate could decrease bilirubin and bile acid levels, while acupuncture had no obvious effect on liver function.


Among PCOS women, metabolic abnormalities and hyperandrogenemia impaired different liver function parameters. Clomiphene could decrease the bilirubin and bile acid levels while acupuncture had no obvious effect on liver function.


PCOS Liver function Metabolic syndrome Hyperandrogenemia Ovulation induction 



The content is solely the responsibility of the authors and does not necessarily represent the official views of the State Administration of Traditional Chinese Medicine of People’s Republic of China. The Steering Committee (SC) members included Xiao-Ke Wu, Jiang-Ping Liu, Tai-Xiang Wu, Ernest HY Ng, Elisabet Stener-Victorin and Heping Zhang, and Richard S Legro (Chair). The Data and Safety Monitoring Board (DSMB) members included Esther Eisenberg, Wei-Liang Weng, Su-Lun Sun, Wei Zou and Zi-Dan Chen, and Robert Rebar (Chair). Meizhuo Zhang in Yale, contributed to the randomization scheme and training of our study personnel. Jin-Ying Fu, Chang-Ling Zhu and Xiao-Hong Wang participated in the patient recruitment at local sites of Henan, Wenzhou and Xuzhou. Other personnel with administrative resource supports included Song-Jiang Liu, Gui-Yuan Wang, Yan-Qiu Du, Yang Xia, Shu-Lai Li, Ke-Qiu Zhang, and Jian-Hua Shen. Yan Li, Wen-Juan Shen, Wei Li and Jing Cong were involved in protocol preparation and blood sample management in Harbin office and core laboratory. We thank the Reproductive Medicine Network Steering Committee of the National Institutes of Health for sharing the protocol and case-report forms from the Pregnancy in Polycystic Ovary Syndrome II study.


National Public Welfare Projects for Chinese Medicine (201107005, 200807002), the National Key Discipline of Chinese Medicine in Gynecology during the year of 2009–2016, (JC200804), the Intervention for Polycystic Ovary Syndrome Based on Traditional Chinese Medicine Theory—‘Tian Gui Disorder’ (2011TD006), and the National Clinical Trial Base in Chinese Medicine Special Projects (JDZX2012036, 2015B009) during the year of 2009–2016 for the First Affiliated Hospital, Heilongjiang University of Chinese Medicine, as well as the Heilongjiang Province ‘Longjiang Scholar’ Program to Xiao-Ke Wu.

Compliance with ethical standards

Conflict of interest

All authors had no conflict of interest to declare.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© Italian Society of Endocrinology (SIE) 2018

Authors and Affiliations

  • W.-Y. Cai
    • 1
  • J.-S. Gao
    • 2
  • X. Luo
    • 1
  • H.-L. Ma
    • 2
  • H. Ge
    • 1
  • N. Liu
    • 1
  • Q. Xia
    • 1
  • Y. Wang
    • 2
  • B.-W. Han
    • 1
  • X.-K. Wu
    • 2
    Email author
  1. 1.Heilongjiang University of Chinese MedicineHarbinChina
  2. 2.Department of Obstetrics and Gynecology, First Affiliated HospitalHeilongjiang University of Chinese MedicineHarbinChina

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