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Journal of Endocrinological Investigation

, Volume 42, Issue 1, pp 27–35 | Cite as

Effects of growth hormone on pregnancy rates of patients with thin endometrium

  • N. Cui
  • A.-M. Li
  • Z.-Y. Luo
  • Z.-M. Zhao
  • Y.-M. Xu
  • J. Zhang
  • A.-M. Yang
  • L.-L. Wang
  • G.-M. HaoEmail author
  • B.-L. GaoEmail author
Original Article

Abstract

Purpose

To investigate whether growth hormone (GH) could improve pregnancy rates of patients with thin endometrium by clinical study and laboratory experiments.

Materials and methods

Ninety-three patients were randomized to either the GH-received group (40) or the routine exogenous administration of estrogens control group (53) for clinical study. The human endometrial carcinoma cell line RL95-2 was used for testing the role of GH with Western blot and real-time PCR by exposure to various concentrations of GH (0.1 nM,1 nM,10 nM,100 nM).

Results

Patients treated with GH had a significantly (P < 0.05) greater endometrium thickness on day 3 (7.87±0.72 vs 6.34±0.86), higher implantation rates (24.4% vs 10.5%) and greater clinical pregnancy rates (42.5% vs 18.9%) compared with the control group. No adverse events were associated with the use of GH. Administration of GH significantly up-regulated the expression of VEGF, ItgB3 and IGF-I expression in RL95-2 cells at both mRNA and protein levels (P < 0.05). AG490, an inhibitor of JAK2, nearly completely inhibited the up-regulative effect of GH through the JAK2-STAT5 pathway, and GH-induced effects could be mediated through autocrine IGF-I together with its hepatic counterpart. IGF-I mRNA was detected in the RL95-2 cells.

Conclusion

GH may improve pregnancy outcomes of patients with thin endometrium who undergo frozen embryo transfer by acting on human endometrial cells to promote proliferation and vascularization and to up-regulate receptivity-related molecular expression.

Keywords

Endometrial thickness Growth hormone Pregnancy rate Cell experiment Mechanism 

Notes

Funding

This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector.

Compliance with ethical standards

Conflict of interest

We declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Italian Society of Endocrinology (SIE) 2018

Authors and Affiliations

  • N. Cui
    • 1
  • A.-M. Li
    • 1
  • Z.-Y. Luo
    • 1
  • Z.-M. Zhao
    • 1
  • Y.-M. Xu
    • 1
  • J. Zhang
    • 1
  • A.-M. Yang
    • 1
  • L.-L. Wang
    • 1
  • G.-M. Hao
    • 1
    Email author
  • B.-L. Gao
    • 2
    Email author
  1. 1.Department of Reproductive Medicine, The Second HospitalHebei Medical UniversityShijiazhuangPeople’s Republic of China
  2. 2.Department of Interventional TherapyHenan Provincial People’s HospitalZhengzhouPeople’s Republic of China

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