Journal of Endocrinological Investigation

, Volume 39, Issue 12, pp 1425–1434 | Cite as

Association between type 1, type 2 cytokines, diabetic autoantibodies and 25-hydroxyvitamin D in children with type 1 diabetes

  • I. M. Talaat
  • A. Nasr
  • A. A. Alsulaimani
  • H. Alghamdi
  • K. A. Alswat
  • D. M. Almalki
  • A. Abushouk
  • A. M. Saleh
  • G. AllamEmail author
Original Article



Vitamin D may play a role in the pathogenesis of type 1 diabetes (T1D). The aim of the current study was to determine the possible association between 25-hydroxyvitamin D [25(OH)D] levels and circulating levels of type 1 and type 2 cytokines, as well as the pathophysiology of T1D in children.


A total of 250 T1D patients and 250 sex- and age-matched T1D-free controls were screened for 25(OH)D, hemoglobin A1c (HbA1c), type 1 and type 2 cytokines, C-reactive protein (CRP) and bone mineral metabolism, as well as antibodies against insulin, glutamic acid decarboxylase (anti-GAD 65) and islet cells.


Our data showed that the plasma level of 25(OH)D was significantly lower in T1D patients and that there was a significant negative correlation between 25(OH)D levels and HbA1c values. There was a significant association between deficient levels of 25(OH)D and higher levels of cytokines (IFN-γ, TNF-α, IL-6, IL-1β, IL-4 and IL-10) and CRP. Total blood hemoglobin, the hematocrit percentage, body mass index SDS values, phosphate and magnesium levels were significantly lower in T1D patients than in T1D-free subjects. The levels of parathyroid hormone and alkaline phosphatase were significantly higher in T1D patients. Higher levels of cytokines were significantly associated with deficient levels of 25(OH)D. Moreover, in T1D patients, higher levels of islet antibodies, anti-GAD antibodies and anti-insulin antibodies were significantly associated with deficient levels of 25(OH)D.


In type 1 diabetic children, deficient levels of 25(OH)D are associated with high levels of HbA1c, circulatory cytokines and antibody markers.


Vitamin D Type 1 diabetes Pathogenesis Cytokines Antibodies Immunomodulation 



We are grateful to the donors and their families for their participation in this study. This work was supported by a research Grant from Taif University, Kingdom of Saudi Arabia (Number: 2-433-1777).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The work described here was performed in accordance with the Declaration of Helsinki. Ethical approval was obtained from the Research Ethics Committee of the Armed Forces Hospitals in Taif, Saudi Arabia.

Informed consent

Informed consent was obtained from the children and their responsible guardians before participation.


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Copyright information

© Italian Society of Endocrinology (SIE) 2016

Authors and Affiliations

  • I. M. Talaat
    • 1
  • A. Nasr
    • 2
    • 3
  • A. A. Alsulaimani
    • 4
  • H. Alghamdi
    • 5
  • K. A. Alswat
    • 6
  • D. M. Almalki
    • 5
  • A. Abushouk
    • 2
  • A. M. Saleh
    • 7
  • G. Allam
    • 8
    • 9
    Email author
  1. 1.Department of Pediatrics, Faculty of MedicineAin Shams UniversityCairoEgypt
  2. 2.Department of Basic Medical Sciences, College of MedicineKSAU-HS-RiyadhRiyadhSaudi Arabia
  3. 3.Department of Microbiology, Faculty of Science and TechnologyAl-Neelain UniversityKhartoumSudan
  4. 4.Department of Pediatrics, College of MedicineTaif UniversityTaifSaudi Arabia
  5. 5.Diabetic CenterPrince Mansour Military Community HospitalTaifSaudi Arabia
  6. 6.Department of Internal Medicine, College of MedicineTaif UniversityTaifSaudi Arabia
  7. 7.Department of Clinical Laboratory Sciences, College of Applied Medical SciencesKSAU-HS-JeddahJeddahSaudi Arabia
  8. 8.Department of Microbiology and Immunology, College of MedicineTaif UniversityTaifSaudi Arabia
  9. 9.Immunology Section, Department of Zoology, Faculty of ScienceBeni-Suef UniversityBeni-SuefEgypt

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