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Journal of Endocrinological Investigation

, Volume 37, Issue 11, pp 1065–1072 | Cite as

Trained and untrained males show reliable salivary testosterone responses to a physical stimulus, but not a psychological stimulus

  • B. T. Crewther
  • L. P. Kilduff
  • C. J. Cook
Original Article

Abstract

Background

The testosterone (T) responses to a physical stimulus are thought to be more stable and reproducible compared to a psychological stimulus.

Purpose

This study compared the salivary T (Sal-T) responses to both stimuli in four groups of men: professional rugby players (n = 17), recreational rugby players (n = 10), a mixed athlete group (n = 14) and untrained controls (n = 12).

Methods

Each group completed three treatments: (1) watching a video with aggressive rugby footage, (2) performing a short bout of sprint exercise and (3) a control session. Saliva samples were taken before and 15 min after each treatment.

Results

The sprint exercise changes in Sal-T levels were similar in the elite rugby (17.1 ± 11.1 %), recreational rugby (11.9 ± 15.9 %), mixed athlete (27.6 ± 32.0 %) and control groups (25.3 ± 23.6 %). In response to the video, Sal-T increased in the elite rugby (6.9 ± 6.4 %) and untrained groups (11.9 ± 13.5 %), but decreased in the recreational rugby players (−7.5 ± 11.0 %). The individual Sal-T responses to the sprints were also correlated (r = 0.69 to 0.82) with other treatment responses.

Conclusions

Sprint exercise had a more consistent effect on Sal-T than a video with aggressive content and thus, could provide a reliable stimulus for increasing T availability in men with different training backgrounds. Individual Sal-T reactivity also appears to be somewhat stable across different treatments. These data provide further understanding around the induction, moderation and interpretation of T physiology.

Keywords

Behaviour Athletes Neuroendocrine Hormones Saliva Video 

Notes

Acknowledgments

We thank the participants, researchers and trainers who contributed to this study. This project was supported by the Elite Sport Performance Research in Training with Pervasive Sensing Programme [EP/H009744/1], funded by the Engineering and Physical Sciences Research Council, and the UK Sports Council.

Conflict of interest

The authors declared no conflict of interests.

References

  1. 1.
    Kraemer WJ, Ratamess NA (2005) Hormonal responses and adaptations to resistance exercise and training. Sports Med 35:339–361PubMedCrossRefGoogle Scholar
  2. 2.
    West DW, Burd NA, Tang JE, Moore DR, Staples AW, Holwerda AM et al (2010) Elevations in ostensibly anabolic hormones with resistance exercise enhance neither training-induced muscle hypertrophy nor strength of the elbow flexors. J Appl Physiol 108:60–67PubMedCrossRefPubMedCentralGoogle Scholar
  3. 3.
    West DW, Kujbida GW, Moore DR, Atherton P, Burd NA, Padzik JP et al (2009) Resistance exercise-induced increases in putative anabolic hormones do not enhance muscle protein synthesis or intracellular signalling in young men. J Physiol 587:5239–5247PubMedCrossRefPubMedCentralGoogle Scholar
  4. 4.
    Crewther BT, Cook C, Cardinale M, Weatherby RP, Lowe T (2011) Two emerging concepts for elite athletes: the short-term effects of testosterone and cortisol on the neuromuscular system and the dose–response training role of these endogenous hormones. Sports Med 41:103–123PubMedCrossRefGoogle Scholar
  5. 5.
    Hellhammer DH, Hubert W, Schürmeyer T (1985) Changes in saliva testosterone after psychological stimulation in men. Psychoneuroendocrino 10:77–81CrossRefGoogle Scholar
  6. 6.
    Stoléru SG, Ennaji A, Cournot A, Spira A (1993) LH pulsatile secretion and testosterone blood levels are influenced by sexual arousal in human males. Psychoneuroendocrino 18:205–218CrossRefGoogle Scholar
  7. 7.
    Fukui H, Yamashita M (2003) The effects of music and visual stress on testosterone and cortisol in men and women. Neuroendocrinol Lett 24:173–180PubMedGoogle Scholar
  8. 8.
    Carré JM, Putnam SK (2010) Watching a previous victory produces an increase in testosterone among elite hockey players. Psychoneuroendocrino 35:475–479CrossRefGoogle Scholar
  9. 9.
    Cook CJ, Crewther BT (2012) Changes in salivary testosterone concentrations and subsequent voluntary squat performance following the presentation of short video clips. Horm Behav 61:17–22PubMedCrossRefGoogle Scholar
  10. 10.
    Kilduff LP, Hopp RN, Cook CJ, Crewther BT, Manning JT (2013) Digit ratio (2D:4D), aggression, and testosterone in men exposed to an aggressive visual stimulus. Evol Psychol 11:953–964PubMedGoogle Scholar
  11. 11.
    Viru AM, Hackney AC, Välja E, Karelson K, Janson T, Viru M (2001) Influence of prolonged continuous exercise on hormone responses to subsequent exercise in humans. Eur J Appl Physiol 85:578–585PubMedCrossRefGoogle Scholar
  12. 12.
    Hackney AC, Hosick KP, Myer A, Rubin DA, Battaglini CL (2012) Testosterone responses to intensive interval versus steady-state endurance exercise. J Endocrinol Invest 35:947–950PubMedCrossRefGoogle Scholar
  13. 13.
    Meckel Y, Nemet D, Bar-Sela S, Radom-Aizik S, Cooper DM, Sagiv M et al (2011) Hormonal and inflammatory responses to different types of sprint interval training. J Strength Cond Res 25:2161–2169PubMedCrossRefGoogle Scholar
  14. 14.
    Sgrò P, Romanelli F, Felici F, Sansone M, Bianchini S, Buzzachera CF et al (2014) Testosterone responses to standardized short-term sub-maximal and maximal endurance exercises: issues on the dynamic adaptive role of the hypothalamic–pituitary–testicular axis. J Endocrinol Invest 37:13–24PubMedCrossRefGoogle Scholar
  15. 15.
    Hansen S, Kvorning T, Kjaer M, Sjøgaard G (2001) The effect of short-term strength training on human skeletal muscle: the importance of physiologically elevated hormone levels. Scand J Med Sci Sport 11:347–354CrossRefGoogle Scholar
  16. 16.
    Beaven MC, Gill ND, Cook CJ (2008) Salivary testosterone and cortisol responses in professional rugby players after four resistance exercise protocols. J Strength Cond Res 22:426–432PubMedCrossRefGoogle Scholar
  17. 17.
    Goto K, Ishii N, Kurokawa K, Takamatsu K (2007) Attenuated growth hormone response to resistance exercise with prior sprint exercise. Med Sci Sports Exerc 39:108–115PubMedCrossRefGoogle Scholar
  18. 18.
    Smith AA, Toone R, Peacock O, Drawer S, Stokes KA, Cook CJ (2013) Dihydrotestosterone is elevated following sprint exercise in healthy young men. J Appl Physiol 114:1435–1440PubMedCrossRefGoogle Scholar
  19. 19.
    Derbré F, Vincent S, Maitel B, Jacob C, Delamarche P, Delamarche A et al (2010) Androgen responses to sprint exercise in young men. Int J Sports Med 31:291–297PubMedCrossRefGoogle Scholar
  20. 20.
    Crewther BT, Cook CJ, Lowe TE, Weatherby RP, Gill N (2011) The effects of short cycle sprints on power, strength and salivary hormones in elite rugby players. J Strength Cond Res 25:32–39PubMedCrossRefGoogle Scholar
  21. 21.
    Paton CD, Lowe T, Irvine A (2010) Caffeinated chewing gum increases repeated sprint performance and augments increases in testosterone in competitive cyclists. Eur J Appl Physiol 110:1243–1250PubMedCrossRefGoogle Scholar
  22. 22.
    Edwards DA, Casto KV (2013) Women’s intercollegiate athletic competition: cortisol, testosterone, and the dual-hormone hypothesis as it relates to status among teammates. Horm Behav 64:153–160PubMedCrossRefGoogle Scholar
  23. 23.
    Jensen J, Oftebro H, Breigan B, Johnsson A, Ohlin K, Meen HD et al (1991) Comparison of changes in testosterone concentrations after strength and endurance exercise in well trained men. Eur J Appl Physiol Occ Physiol 63:467–471CrossRefGoogle Scholar
  24. 24.
    Hackney AC, Viru A (2008) Research methodology: endocrinologic measurements in exercise science and sports medicine. J Athl Train 43:631–639PubMedCrossRefPubMedCentralGoogle Scholar
  25. 25.
    Bird SP, Tarpenning KM (2004) Influence of circadian time structure on acute hormonal responses to a single bout of heavy-resistance exercise in weight-trained men. Chronobiol Int 21:131–146PubMedCrossRefGoogle Scholar
  26. 26.
    Maestripieri D, Baran NM, Sapienza P, Zingales L (2010) Between- and within-sex variation in hormonal responses to psychological stress in a large sample of college students. Stress 13:413–424PubMedCrossRefGoogle Scholar
  27. 27.
    Beaven MC, Ingram JR, Gill ND, Hopkins WG (2010) Ultradian rhythmicity and induced changes in salivary testosterone. Eur J Appl Physiol 110:405–413PubMedCrossRefGoogle Scholar
  28. 28.
    DeSoto CM, Hitlan RT, Deol RS, McAdams D (2009) Testosterone fluctuations in young men: the difference between interacting with like and not-like others. Evol Psychol 8:173–188Google Scholar
  29. 29.
    Crewther BT, Lowe T, Ingram J, Weatherby RP (2010) Validating the salivary testosterone and cortisol concentration measures in response to short high-intensity exercise. J Sports Med Phys Fit 50:85–92Google Scholar
  30. 30.
    Gibson LE, Checkley S, Papadopoulos A, Poon L, Daley S, Wardle J (1999) Increased salivary cortisol reliable induced by a protein-rich midday meal. Psychosom Med 61:214–224PubMedCrossRefGoogle Scholar
  31. 31.
    Maresh CM, Whittlesey MJ, Armstrong LE, Yamamoto LM, Judelson DA, Fish KE et al (2006) Effect of hydration state on testosterone and cortisol responses to training-intensity exercise in collegiate runners. Int J Sports Med 27:765–770PubMedCrossRefGoogle Scholar
  32. 32.
    Buresh R, Berg K, French J (2009) The effect of resistive exercise rest interval on hormonal response, strength, and hypertrophy with training. J Strength Cond Res 23:62–71PubMedCrossRefGoogle Scholar
  33. 33.
    Pirke KM, Kockott G, Dittmar F (1974) Psychosexual stimulation and plasma testosterone in man. Arch Sexual Behav 3:577–584CrossRefGoogle Scholar
  34. 34.
    Budde H, Pietrassyk-Kendziorra S, Bohm S, Voelcker-Rehage C (2010) Hormonal responses to physical and cognitive stress in a school setting. Neurosci Lett 474:131–134PubMedCrossRefGoogle Scholar
  35. 35.
    Bouarfa L, Bembnowicz P, Crewther B, Jarchi D, Yang G-Z (2013) Profiling visual and verbal stress responses using electrodermal, heart rate and hormonal measures. IEEE Body Sensor Network Conference. MIT, Cambridge, Massachusetts p 1–7Google Scholar
  36. 36.
    Schoofs D, Wolf OT (2011) Are salivary gonadal steroid concentrations influenced by acute psychological stress? A study using the Trier Social Stress Test (TSST). Int J Psychophysiol 80:36–43PubMedCrossRefGoogle Scholar
  37. 37.
    Heinz A, Hermann D, Smolka MN, Rieks M, Gräf KJ, Pöhlau D et al (2003) Effects of acute psychological stress on adhesion molecules, interleukins and sex hormones: implications for coronary heart disease. Psychopharmacology 165:111–117PubMedGoogle Scholar
  38. 38.
    Lennartsson A-K, Kushnir MM, Bergquist J, Billig H, Jonsdottir IH (2012) Sex steroid levels temporarily increase in response to acute psychosocial stress in healthy men and women. Int J Psychophysiol 84:246–253PubMedCrossRefGoogle Scholar
  39. 39.
    Cook CJ, Crewther BT (2012) The effects of different pre-game motivational interventions on athlete free hormonal state and subsequent performance in professional rugby union matches. Physiol Behav 106:683–688PubMedCrossRefGoogle Scholar
  40. 40.
    Marceau K, Shirtcliff EA, Hastings PD, Klimes-Dougan B, Zahn-Waxler C, Dorn LD et al (2014) Within-adolescent coupled changes in cortisol with DHEA and testosterone in response to three stressors during adolescence. Psychoneuroendocrino 41:33–45CrossRefGoogle Scholar
  41. 41.
    Edwards DA, Kurlander LS (2010) Women’s intercollegiate volleyball and tennis: effects of warm-up, competition, and practice on saliva levels of cortisol and testosterone. Horm Behav 58:606–613PubMedCrossRefGoogle Scholar
  42. 42.
    Tsopanakis A, Stalikas A, Sgouraki E, Tsopanakis C (1994) Stress adaptation in athletes: relation of lipoprotein levels to hormonal response. Pharmacol Biochem Behav 48:377–382PubMedCrossRefGoogle Scholar
  43. 43.
    Beaven MC, Cook CJ, Gill ND (2008) Significant strength gains observed in rugby players after specific resistance exercise protocols based on individual salivary testosterone responses. J Strength Cond Res 22:419–425PubMedCrossRefGoogle Scholar

Copyright information

© Italian Society of Endocrinology (SIE) 2014

Authors and Affiliations

  • B. T. Crewther
    • 1
    • 3
  • L. P. Kilduff
    • 2
  • C. J. Cook
    • 1
    • 2
    • 3
  1. 1.Hamlyn Centre for Robotic SurgeryImperial College South Kensington CampusLondonUK
  2. 2.A-STEM, College of EngineeringSwansea UniversitySwanseaUK
  3. 3.School of Sport Health and Exercise Science Bangor UniversityBangorUK

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