Aging Clinical and Experimental Research

, Volume 30, Issue 11, pp 1391–1398 | Cite as

Effects of Psidium guajava Linn. leaf extract in Japanese subjects with knee pain: a randomized, double-blind, placebo-controlled, parallel pilot study

  • Shingo Kakuo
  • Takashi Fushimi
  • Keiko Kawasaki
  • Junji Nakamura
  • Noriyasu OtaEmail author
Original Article


The extract of Psidium guajava Linn. (guava) leaf was recently revealed to suppress the advance of osteoarthritis (OA) in rat anterior cruciate ligament-transection models. To investigate the efficacy of guava leaf extract in improving knee pain, which is a common symptom of OA, we conducted a double-blind parallel pilot clinical study in Japanese subjects with knee pain. The subjects, who had no medical history of knee treatment, were randomly assigned to two groups with similar total Japanese Knee Osteoarthritis Measure (JKOM) scores. During the 12-week intake period, the subjects in each group ingested 1 g of guava leaf extract (the guava group) or placebo (the placebo group) daily. At week 12, pain and stiffness in knees (one subcategory of JKOM score) in the guava group was significantly lower than that in the placebo group. Using the visual analogue scale (VAS) for knee pain, a significant association between treatment effect and test period was shown, and the guava group had a lower VAS score at week 12 than the placebo group. In conclusion, continuous intake of guava leaf extract might relieve knee pain, suggesting a potential preventive effect against OA symptoms.


Guava leaf extract Knee pain Osteoarthritis JKOM VAS 



This study was funded by Kao Corporation. All authors; S. Kakuo, T. Fushimi, K. Kawasaki, J. Nakamura and N. Ota are employees of this company. We would like to thank Editage ( for English language editing.

Author contributions

SK, JN and NO designed this study. SK and TF analyzed data. SK, TF and NO interpreted the results. KK prepared test samples. TF and NO drafted this manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest associated with this manuscript.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Ethics Committee of Kao Corporation and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. The present study protocol was registered in the University Hospital Medical Information Network (UMIN), one of the ICMJE-accredited clinical trial sites (ID: UMIN 000031558). This article does not contain any studies with animals performed by any of the authors.

Informed consent

Written informed consent for participation was obtained from all individual participants in the study.


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Shingo Kakuo
    • 1
  • Takashi Fushimi
    • 1
  • Keiko Kawasaki
    • 1
  • Junji Nakamura
    • 2
  • Noriyasu Ota
    • 1
    Email author
  1. 1.Biological Science Research LaboratoriesKao CorporationHaga-gunJapan
  2. 2.Kansei Science Research LaboratoriesKao CorporationHaga-gunJapan

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