Aging Clinical and Experimental Research

, Volume 28, Issue 4, pp 625–632

The role of protein oxidation and DNA damage in elderly hypertension

  • Serap Yavuzer
  • Hakan Yavuzer
  • Mahir Cengiz
  • Hayriye Erman
  • Filiz Demirdag
  • Alper Doventas
  • Huriye Balci
  • Deniz Suna Erdincler
  • Hafize Uzun
Original Article

Abstract

Introduction

This study aimed to evaluate the role of protein oxidation and DNA damage in the elderly hypertensive (HT) patients.

Materials and methods

This study consisted of four groups: two elderly groups with 30 HT patients and 30 normotensive healthy volunteers, and two young groups with 30 HT patients and 30 normotensive healthy volunteers. Plasma total thiol (T-SH), advanced oxidation protein products (AOPPs), protein carbonyl (PCO), ischemia modified albumin (IMA), urine 8-hydroxy-2′-deoxyguanosine (8-OHdG), and prooxidant–antioxidant balance (PAB) levels were measured.

Results

In the elderly HT group AOPPs, PCO, 8-OHdG, and PAB were significantly higher than the elderly control group. In the young HT group T-SH levels were significantly lower and the other oxidative stress parameters were significantly higher than the young control group. In the elderly control group AOPPs, PCO, IMA, 8-OHdG and PAB were significantly higher than the young control group. T-SH was significantly lower in the elderly control than the young control group. In the elderly HT group, T-SH levels were significantly lower and AOPPs, PCO, IMA, 8-OHdG, and PAB levels were significantly higher than the young HT group.

Conclusion

Protein and DNA cell damage occurs by oxidation of free radicals throughout life. Our study supports the view that these radicals may be responsible for the development of hypertension with aging process. Urine 8-OHdG levels can be used as a marker for oxidative DNA damage in the elderly hypertensive patients. Finally, our results suggest that oxidative stress may influence both the development and progression of hypertension and aging.

Keywords

Aging Hypertension Oxidative stress Protein oxidation DNA damage 

References

  1. 1.
    Logan AG (2011) Hypertension in aging patients. Expert Rev Cardiovasc Ther 9:113–120CrossRefPubMedGoogle Scholar
  2. 2.
    Cakatay U, Kayali R, Uzun H (2008) Relation of plasma protein oxidation parameters and paraoxonase activity in the ageing population. Clin Exp Med 8:51–57CrossRefPubMedGoogle Scholar
  3. 3.
    Beckman KB, Ames BN (1998) The free radical theory of aging matures. Physiol Rev 78:547–581PubMedGoogle Scholar
  4. 4.
    Rubio-Ruiz ME, Pérez-Torres I, Soto ME et al (2014) Aging in blood vessels. Medicinal agents FOR systemic arterial hypertension in the elderly. Ageing Res Rev 18:132–147CrossRefPubMedGoogle Scholar
  5. 5.
    Reaven PD, Napoli C, Merat S et al (2000) Lipoprotein modification and atherosclerosis in aging. Exp Gerontol 34:527–537CrossRefGoogle Scholar
  6. 6.
    Seres I, Paragh G, Deschene E et al (2004) Study of factors influencing the decreased HDL associated PON1 activity with aging. Exp Gerontol 39:59–66CrossRefPubMedGoogle Scholar
  7. 7.
    Dincer Y, Sekercioglu N, Pekpak M et al (2008) Assessment of DNA oxidation and antioxidant activity in hypertensive patients with chronic kidney disease. Ren Fail 30(10):1006–1011CrossRefPubMedGoogle Scholar
  8. 8.
    Hu ML (1994) Measurement of protein thiol groups and glutathione in plasma. Methods Enzymol 233:381–385Google Scholar
  9. 9.
    Gelisgen R, Genc H, Kayali R et al (2011) Protein oxidation markers in women with and without gestational diabetes mellitus: a possible relation with paraoxonase activity. Diabetes Res Clin Pract 94:404–409CrossRefPubMedGoogle Scholar
  10. 10.
    Alamdari DH, Ghayour-Mobarhan M, Tavallaie S et al (2008) Prooxidant-antioxidant balance as a new risk factor in patients with angiographically defined coronary artery disease. Clin Biochem 41:375–380CrossRefPubMedGoogle Scholar
  11. 11.
    American Diabetes Association (2003) Diabetic nephropathy. Diabetes Care 26(Suppl 1):S94–S98Google Scholar
  12. 12.
    Nakamoto H, Kaneko T, Tahara S et al (2007) Regular exercise reduces 8-oxodG in the nuclear and mitochondrial DNA and modulates the DNA repair activity in the liver of old rats. Exp Gerontol 42:287–295CrossRefPubMedGoogle Scholar
  13. 13.
    Berlett BS, Stadtman ER (1997) Protein oxidation in aging, disease, and oxidative stress. J Biol Chem 272:20313–20316CrossRefPubMedGoogle Scholar
  14. 14.
    Stadtman ER (2001) Protein oxidation in aging and age-related diseases. Ann N Y Acad Sci 928:22–38CrossRefPubMedGoogle Scholar
  15. 15.
    Çakatay U, Kayali R (2005) Plasma protein oxidation in aging rats after alpha-lipoic acid administration. Biogerontology 6:87–93CrossRefPubMedGoogle Scholar
  16. 16.
    Young IS, Woodside JV (2001) Antioxidants in health and disease. J Clin Pathol 54:176–186CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Salive ME, Cornoni-Huntley J, Phillips CL et al (1992) Serum albumin in older persons: relationship with age and health status. J Clin Epidemiol 45:213–221CrossRefPubMedGoogle Scholar
  18. 18.
    Gom I, Fukushima H, Shiraki M et al (2007) Relationship between serum albumin level and aging in community-dwelling self-supported elderly population. J Nutr Sci Vitaminol 53:37–42CrossRefPubMedGoogle Scholar
  19. 19.
    Campion EW, deLabry LO, Glynn RJ (1988) The effect of age on serum albumin in healthy males: report from the Normative Aging Study. J Gerontol 43:M18–M20CrossRefPubMedGoogle Scholar
  20. 20.
    Caner M, Karter Y, Uzun H et al (2006) Oxidative stress in human in sustained and white coat hypertension. Int J Clin Pract 60:1565–1571CrossRefPubMedGoogle Scholar
  21. 21.
    Agarwal S, Sohal RS (1993) Relationship between aging and susceptibility to protein oxidative damage. Biochem Biophys Res Commun 194:1203–1206CrossRefPubMedGoogle Scholar
  22. 22.
    Sohal RS, Agarwal S, Sohal BH (1995) Oxidative stress and aging in the Mongolian gerbil (Meriones unguiculatus). Mech Ageing Dev 81:15–25CrossRefPubMedGoogle Scholar
  23. 23.
    Piwowar A, Knapik-Kordecka M, Warwas M (2008) Ischemia-modified albumin level in type 2 diabetes mellitus: preliminary report. Dis Markers 24:311–317CrossRefPubMedPubMedCentralGoogle Scholar
  24. 24.
    Roy D, Quiles J, Gaze DC et al (2006) Role of reactive oxygen species on the formation of the novel diagnostic marker ischaemia modified albumin. Heart 92:113–114CrossRefPubMedPubMedCentralGoogle Scholar
  25. 25.
    Toker A, Mehmetoglu I, Yerlikaya FH et al (2013) Investigation of oxidative stress markers in essential hypertension. Clin Lab 59:107–114PubMedGoogle Scholar
  26. 26.
    Kim JY, Prouty LA, Fang SC et al (2009) Association between fine particulate matter and oxidative DNA damage may be modified in individuals with hypertension. J Occup Environ Med 51:1158–1166CrossRefPubMedGoogle Scholar
  27. 27.
    Negishi H, Ikeda K, Kuga S et al (2001) The relation of oxidative DNA damage to hypertension and other cardiovascular risk factors in Tanzania. J Hypertens 19:529–533CrossRefPubMedGoogle Scholar
  28. 28.
    Loft S, Vistisen K, Ewertz M et al (1992) Oxidative DNA damage estimated by 8-hydroxydeoxyguanosine excretion in humans: influence of smoking, gender and body mass index. Carcinogenesis 132:2241–2247CrossRefGoogle Scholar
  29. 29.
    Subash P, Gurumurthy P, Sarasabharathi A et al (2010) Urinary 8-OHdG: a marker of oxidative stress to DNA and total antioxidant status in essential hypertension with South Indian population. Indian J Clin Biochem 25:127–132CrossRefPubMedPubMedCentralGoogle Scholar
  30. 30.
    Izzo JL Jr (2001) Aging and systolic hypertension: cluster patterns and problem-solving strategies to answer the genetic riddle. Hypertension 37:1067–1068CrossRefPubMedGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Serap Yavuzer
    • 1
  • Hakan Yavuzer
    • 2
  • Mahir Cengiz
    • 1
  • Hayriye Erman
    • 3
  • Filiz Demirdag
    • 2
  • Alper Doventas
    • 2
  • Huriye Balci
    • 4
  • Deniz Suna Erdincler
    • 2
  • Hafize Uzun
    • 3
  1. 1.Department of Internal Medicine, Cerrahpasa Faculty of MedicineIstanbul UniversityIstanbulTurkey
  2. 2.Division of Geriatrics, Department of Internal Medicine, Cerrahpasa Faculty of MedicineIstanbul UniversityIstanbulTurkey
  3. 3.Department of Biochemistry, Cerrahpasa Faculty of MedicineIstanbul UniversityIstanbulTurkey
  4. 4.Central Research Laboratory, Cerrahpasa Faculty of MedicineIstanbul UniversityIstanbulTurkey

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