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Lack of association between FTO gene variations and metabolic healthy obese (MHO) phenotype: Tehran Cardio-metabolic Genetic Study (TCGS)

  • Bahareh Sedaghati-khayat
  • Maryam Barzin
  • Mahdi Akbarzadeh
  • Kamran Guity
  • Mohammad-Sadegh Fallah
  • Hoda Pourhassan
  • Fereidoun Azizi
  • Maryam S. Daneshpour
Original Article

Abstract

Background

Obesity is currently an international epidemic and metabolic derangements pose these individuals at greater risk for future morbidity and mortality. Genetics and environmental factors have undeniable effects and among genetic risk factors, FTO/CETP genes are important. The current study examines the interaction between obesity phenotypes and FTO/CETP SNPs and their effects on lipid profile changes.

Materials and methods

We selected 954 adult subjects from TCGS (47.9% male). Participants were stratified according to their BMI and presence of metabolic syndrome according to the Joint Interim Statement (JIS) definition. Nine selected polymorphisms from FTO/CETP genes were genotyped using Tetra ARMS-PCR method. After age and sex adjustment the interaction of 9 markers with lipid profiles among phenotypes were tested by PASW.

Results

In three main groups, HDL_C level had a strong significant association with CETP markers: (rs3764261, β(95% CI) − 0.48(− 0.61 to − 0.35), P = 1.0 × 10−11), (rs1800775, β(95% CI) 0.5(0.36;0.65), P = 1.0 × 10−6) and (rs1864163, β(95% CI) 0.3(0.16;0.43), P = 9.1 × 10−5). This association was also seen in rs7202116 within the total population. In only unhealthy metabolic obese (MUHO) subgroups four new FTO markers (rs1421085, rs1121980, rs1558902 and rs8050136) (P value < 0.01) demonstrated significant association, even after lipid profile adjustment.

Conclusion

In the present study, we investigated the association between obesity phenotypes and some variations in FTO/CETP genes for the first time. Our study showed that four markers in the first intron of the FTO gene should be the risk marker in MUHO participants.

Level of Evidence

Level III, case-control study

Keywords

Obesity Metabolic syndrome Fat mass and obesity-associated protein Cholesteryl ester transfer protein 

Notes

Acknowledgements

The study was done under supervision of Cellular and Molecular Endocrine Research Center and Obesity research center in the Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Funding

This study supported by the Iran National Scientific Foundation, Tehran, Iran (Grant number 93017278).

Compliance with ethical standards

Conflict of interest

The authors declare that they do not have any conflict of interests.

Ethics approval

The study protocol was approved by the ethics committee of the Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Informed consent

Written consent was obtained from each subject.

Consent for publication.

Not applicable.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Bahareh Sedaghati-khayat
    • 1
  • Maryam Barzin
    • 2
  • Mahdi Akbarzadeh
    • 1
  • Kamran Guity
    • 1
  • Mohammad-Sadegh Fallah
    • 3
  • Hoda Pourhassan
    • 4
  • Fereidoun Azizi
    • 5
  • Maryam S. Daneshpour
    • 1
  1. 1.Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine SciencesShahid Beheshti University of Medical SciencesTehranIslamic Republic of Iran
  2. 2.Obesity Research Center, Research Institute for Endocrine SciencesShahid Beheshti University of Medical SciencesTehranIslamic Republic of Iran
  3. 3.Kawsar Human Genetics Research CenterTehranIslamic Republic of Iran
  4. 4.Department of Internal MedicineUniversity of California RiversideRiversideUSA
  5. 5.Endocrine Research Center, Research Institute for Endocrine SciencesShahid Beheshti University of Medical SciencesTehranIslamic Republic of Iran

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