Functional annotation of differentially expressed genes under salt stress in Dichanthium annulatum
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Soil salinity is one of the important abiotic stresses affecting plant growth and development. Halophytes can be one of the options to explore the salt tolerance potential and to identify the potential gene(s) which can be used in crop improvement programs. In view of this, the present experiment was conducted on grass halophyte, Dichanthium annulatum, which can tolerate soil salinity up to EC 30 dS/m (~ 300 mM NaCl) to identify the gene(s) for salt tolerance. The de novo assembly generated 267,196 transcripts and these assembled transcripts were further clustered into 188,353 transcripts. An average of 64.47% of the transcripts was functionally annotated against the viridiplantae databases since no genomic reference is available for Dichanthium. Gene ontology and pathways analysis using KAAS database identified that 48.13% transcripts were involved in molecular function, 37.21% in cellular component and 14.66% in biological processes. The annotation of these genes provides a pathway analysis for their putative functions under salt stress conditions.
KeywordsSalt stress Halophytes Dichanthium Gene Salt tolerance
The authors are highly thankful to the Director, ICAR-CSSRI, Karnal for providing necessary facilities to carry out the research work. The first author also sincerely acknowledges the ICAR-National Agricultural Science Fund (NASF), New Delhi for funding this work.
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