Genome-wide identification and expression profiling of genes encoding universal stress proteins (USP) identify multi-stress responsive USP genes in Arabidopsis thaliana
- 2 Downloads
Universal stress proteins (USPs) are stress-responsive proteins conserved among various organisms, including bacteria, plants and metazoans. However, in plants, the function of most of the USPs remains largely unknown. In the present study, we have identified 53 USP domain-containing proteins encoded by 41 genes in the Arabidopsis genome. Based on the presence of additional protein kinase or tyrosine kinase domain, the nomenclature has been provided to these proteins. Comprehensive in silico expression profiling of AtUSPs under various developmental stages revealed that most of the genes are expressed in a tissue-specific manner. Under abiotic stresses, AtUSP9 and AtUSP12 were identified as multi-stress responsive in both shoot and root tissues. Interestingly, AtUSP9 was also induced under various pathogens and elicitor treatments. The expression analysis of USP genes under abiotic stresses using qRT-PCR correlated well with in silico expression analysis. Thus, the present study provides a blueprint for the functional characterization of AtUSPs to ascertain their role under stress conditions. Moreover, AtUSP9 and AtUSP12 may also be used to engineer plants with improved tolerance against multiple stresses.
KeywordsAbiotic stresses Arabidopsis Universal stress protein Multi-stress responsive In silico expression
We thank the Council of Scientific and Industrial Research (CSIR) for funding in the form of network Project PlaGen (BSC0107). MB and PG thanks CSIR for providing JRF and SRF fellowships. This manuscript represents CSIR-IHBT Communication No. 3921.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Ascencio-Ibanez, J. T., Sozzani, R., Lee, T. J., Chu, T. M., Wolfinger, R. D., Cella, R., et al. (2008). Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during Geminivirus infection. Plant Physiology, 148, 436–454.CrossRefGoogle Scholar
- Johnston, A. J., Meier, P., Gheyselinck, J., Wuest, S. E. J., Federer, M., Schlagenhauf, E., et al. (2007). Genetic subtraction profiling identifies genes essential for Arabidopsis reproduction and reveals interaction between the female gametophyte and the maternal sporophyte. Genome Biology, 8, R204.CrossRefGoogle Scholar
- Jost, R., Pharmawati, M., Lapiz-Gaza, H. R., Rossig, C., Berkowitz, O., et al. (2015). Differentiating phosphate-dependent and phosphate independent systemic phosphate-starvation response networks in Arabidopsis thaliana through the application of phosphate. Journal of Experimental Botany, 66, 1–14.CrossRefGoogle Scholar
- Jung, J. J., Melenscion, S. B., Lee, E. S., Park, J. H., Alinapon, V. C., Oh, H. T., et al. (2015). Universal stress protein exhibits a redox-dependent chaperone function in Arabidopsis and enhances plant tolerance to heat shock and oxidative stress. Frontiers in Plant Science, 6, 1141.PubMedPubMedCentralGoogle Scholar
- Kasukabe, Y., He, L., Nada, K., Misawa, S., Ihara, I., & Tachibana, S. (2004). Overexpression of spermidine synthase enhances tolerance to multiple environmental stresses and up-regulates the expression of various stress-regulated genes in transgenic Arabidopsis thaliana. Plant and Cell Physiology, 45, 712–722.CrossRefGoogle Scholar
- Kushwaha, H. R., Singh, A. K., Sopory, S. K., Singla-Pareek, S. L., & Pareek, A. (2009). Genome-wide expression analysis of CBS domain containing proteins in Arabidopsis thaliana (L.) Heynh and Oryza sativa (L.) reveals their developmental and stress regulation. BMC Genomics, 10, 200.CrossRefGoogle Scholar
- Shafi, A., Chauhan, R., Gill, T., Swarnkar, M. K., Sreenivasulu, Y., Kumar, S., et al. (2015). Expression of SOD and APX genes positively regulates secondary cell wall biosynthesis and promotes plant growth and yield in Arabidopsis under salt stress. Plant Molecular Biology, 87, 615–631.CrossRefGoogle Scholar
- Singh, A. K., Sopory, S. K., Wu, R., & Singla-Pareek, S. L. (2010). Transgenic approaches. In A. Pareek, S. K. Sopory, H. J. Bohnert, & M. A. Govindjee (Eds.), Abiotic stress adaptation in plants: Physiological molecular and genomic foundation (pp. 418–438). Berlin: Springer.Google Scholar
- Yamaguchi-Shinozaki, K., Koizumi, M., Urao, S., & Shinozaki, K. (1992). Molecular cloning and characterization of 9 cDNAs for genes that are responsive to desiccation in Arabidopsis thaliana: Sequence analysis of one cDNA clone that encodes a putative transmembrane channel protein. Plant and Cell Physiology, 33(3), 217–224.CrossRefGoogle Scholar