Quantitative trait loci (QTL) for salinity tolerance traits in interspecific hybrids of Eucalyptus
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Soil salinity is one of the major limiting factors in productivity of plants. Cultivation of industrially important fast growing saline tolerant tree species is one of the options to reclaim the saline soils. Some of the Eucalyptus species are salt tolerant and production of interspecific hybrids of these species would enhance productivity in saline environments. In this study, phenotypic parameters for growth, physiology and mineral nutrition were estimated in Eucalyptus camaldulensis × E. tereticornis F1 hybrids to understand the mechanism of salinity tolerance and localize quantitative trait loci (QTL) involved in sodium chloride (NaCl) stress. Salt injury scoring and plasma membrane damage showed a significant difference between tolerant and susceptible individuals, which was correlated with the gas exchange measurements and Na+, K+ and K+/Na+ ratio. Under salinity, correlation of gas exchange measurements showed strong positive correlations between the traits, Anet, gs, Ci and E indicated the role of stomatal function. It was inferred that sequestration of NaCl by the salt tolerant individuals was through compartmentalization of Na+ and its detoxification by maintenance of K+/Na+ ratio. Totally, 33 QTL were identified under salinity and control conditions. Co-localization of QTL regulating Na+ and K+ transport substantiated their influence in salinity tolerance which could be due to the closely linked genes or by pleiotropic effect of same genes on these traits. Fine mapping with more molecular markers will locate the QTL precisely and validating with field trails could hasten the traditional methods for salinity breeding.
KeywordsEucalyptus NaCl stress SSR markers Quantitative trait loci
The authors acknowledge Indian Council of Forestry Research and Education (ICFRE) for financial support. Senior Research Fellowship provided to V. Subashini by ICFRE is acknowledged.
VSU conducted salt tolerance experiments, SSR genotyping, data analysis and drafted the manuscript. VKWB conducted field establishment and vegetative propagation, AM, BN and VSI carried out the controlled pollination and hybrid establishment, VSI participated in data analysis, RY conceived, organized and planned the research and finalised the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Canavar, O., Gotz, K. P., Ellmer, F., Chmielewski, F. M., & Kaynak, M. A. (2014). Determination of the relationship between water use efficiency, carbon isotope discrimination and proline in sunflower genotypes under drought stress. Australian Journal of Crop Science, 8, 232–242.Google Scholar
- Cha-um, S., Somsueb, S., Samphumphuang, T., & Kirdmanee, C. (2013). Salt tolerant screening in eucalypt genotypes (Eucalyptus spp.) using photosynthetic abilities, proline accumulation, and growth characteristics as effective indices. In Vitro Cellular & Developmental Biology—Plant, 49, 611–619.CrossRefGoogle Scholar
- Chunthaburee, S., Dongsansuk, A., Sanitchon, J., Pattanagul, W., & Theerakulpisut, P. (2016). Physiological and biochemical parameters for evaluation and clustering of rice cultivars differing in salt tolerance at seedling stage. Saudi Journal of Biological Sciences, 23, 467–477.CrossRefGoogle Scholar
- Dale, G., T., Aitken, K., S., & Sasse, J., M. (2000). Development of salt tolerant E. camaldulensis x E. grandis hybrid clones using phenotypic selection and genetic mapping. In: Proceedings of QFRI/CRC-SPF symposium on hybrid breeding and genetics of forest trees. Noosa, Australia (pp. 227–233).Google Scholar
- F. A. O. (2005) Salt-affected soils from sea water intrusion: Strategies for rehabilitation and management. Report of the regional workshop. Bangkok, Thailand, 62 pp.Google Scholar
- Farrell, R. C. C., Bell, D. T., Akilan, K., & Marshall, J. K. (1996). Morphological and physiological comparisons of clonal lines of Eucalyptus camaldulensis. II. Responses to waterlogging/salinity and alkalinity. Australian Journal of Plant Physiology, 23, 509–518.Google Scholar
- Freeman, J. S. (2014). Molecular linkage maps of Eucalyptus: Strategies, resources and achievements. In R. J. Henry & C. Kole (Eds.), Genetics, genomics and breeding of eucalypts (pp. 58–74). Boca Raton: CRC Press.Google Scholar
- Gregorio, G. B., Senadhira, D., & Mendoza, R. D. (1997). Screening rice for salinity tolerance. Laguna Philippines: International Rice Research Institute, 1997, 1–30.Google Scholar
- Madejón, P., Marañón, T., Navarro-Fernández, C. M., Domínguez, M. T., Alegre, J. M., Robinson, B., et al. (2017). Potential of Eucalyptus camaldulensis for phytostabilization and biomonitoring of trace-element contaminated soils. PLoS ONE, 12, e0180240. https://doi.org/10.1371/journal.pone.0180240.CrossRefPubMedPubMedCentralGoogle Scholar
- Mandal, A. K., Sharma, R. C., Singh. G., & Dagar, J.C. (2010) Computerized Database on Salt Affected Soils in India. Technical Bulletin No.2/2010. Central Soil Salinity Research Institute, Karnal pp 28.Google Scholar
- Marcar, N. (2016). Prospects for managing salinity in Southern Australia using trees on farmland. In J. C. Dagar & P. Minhas (Eds.), Agroforestry for the management of waterlogged saline soils and poor-quality waters. Delhi: Springer.Google Scholar
- Marcar, N. E. (1993). Waterlogging Modifies Growth, Water Use and Ion Concentrations in Seedlings of Salt-Treated Eucalyptus camaldulensis, E. tereticornis, E. robusta and E. globulus. Australian Journal of Plant Physiology, 20, 1–13.Google Scholar
- Rangani, J., Parida, A. K., Panda, A., & Kumari, A. (2016). Coordinated changes in antioxidative enzymes protect the photo synthetic machinery from salinity induced oxidative damage and confer salt tolerance in an extreme halophyte Salvadora persica L. Frontiers in Plant Science, 7, 50.CrossRefGoogle Scholar
- Rattan, A., Kapoor, D., Kapoor, N., & Bhardwaj, R. (2014). Application of brassionsteroids reverses the inhibitory effect of salt stress on growth and photosynthetic activity of Zea mays plants. Theoretical and Applied Genetics, 6, 13–22.Google Scholar
- Shanthi, K., Bachpai, V. K., Anisha, S., Ganesan, M., Anithaa, R. G., Subashini, V., et al. (2015). Micropropagation of Eucalyptus camaldulensis for the production of rejuvenated stock plants for microcuttings propagation and genetic fidelity assessment. New Forests, 46, 357–371.CrossRefGoogle Scholar
- Sixto, H., González-González, B. D., Molina-Rueda, J. J., Garrido-Aranda, A., Sanchez, M. M., & López, G., et al. (2016). Eucalyptus spp. and Populus spp. coping with salinity stress: an approach on growth, physiological and molecular features in the context of short rotation coppice (SRC). Trees. https://doi.org/10.1007/s00468-016-1420-7 CrossRefGoogle Scholar
- Stevens, J., Senaratna, T., & Sivasithamparam, K. (2006). Salicylic acid induces salinity tolerance in tomato (Lycopersicon esculentum cv. Roma): Associated changes in gas exchange, water relations and membrane stabilization. Plant Growth Regulation, 49, 77–83.Google Scholar
- Subashini, V., Shanmugapriya, A., & Yasodha, R. (2014). Hybrid purity assessment in Eucalyptus F1 hybrids using microsatellite markers. 3. Biotech, 4, 367–373.Google Scholar