Indian Journal of Plant Physiology

, Volume 23, Issue 3, pp 516–520 | Cite as

Variable polyphenol oxidase (PPO) activity indicates grain quality in bread wheat (Triticum aestivum L.)

  • Neha Salaria
  • Sarvjeet Kukreja
  • Sharanpreet Kaur
  • Umesh GoutamEmail author
Original Article


Wheat (Triticum spp.) is one of the important cereals in the world. Discoloration of wheat grains and accumulation of by product due to polyphenol oxidase (PPO) activities is an important problem faced by food industries. In this study, seeds of 115 Indian bread wheat cultivars representing different six agroclimatic zones were screened for their PPO activities using different PPO substrates, like caffeic acid, catechol, tyrosine and L-DOPA. Among these substrates, L-DOPA was found as best substrate to detect PPO activity. Highest PPO activity with L-DOPA was found in wheat cultivar HS 207, while lowest was detected in HP 1633. Among different agroclimatic zones, seeds of 40% of the cultivars representing NWPZ showed low PPO activity (< 10 unit min−1 g−1) with an average value of 14.22 unit min−1 g−1 and 38% cultivars of NEPZ had low PPO activity with the mean of 13.56 unit min−1 g−1. Therefore, wheat cultivars form NWPZ and NEPZ can be used for producing wheat byproducts.


Bread wheat Polyphenol oxidase Total polyphenol Triticum aestivum L 



Total polyphenol content


Polyphenol oxidase


L-3,4-dihdroxyphenyl alanine


Gallic acid equivalent

Supplementary material

40502_2018_379_MOESM1_ESM.docx (21 kb)
Supplementary material 1 (DOCX 20 kb)
40502_2018_379_MOESM2_ESM.docx (15 kb)
Supplementary material 2 (DOCX 15 kb)


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Copyright information

© Indian Society for Plant Physiology 2018

Authors and Affiliations

  • Neha Salaria
    • 1
  • Sarvjeet Kukreja
    • 2
  • Sharanpreet Kaur
    • 1
  • Umesh Goutam
    • 1
    Email author
  1. 1.Department of BiotechnologyLovely Professional UniversityPhagwaraIndia
  2. 2.Ch. M. R. M. Memorial CollegeSriganganagarIndia

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