Biochemical and physiological response to oxidative stress in cultivated sugarcane and wild genera
- 69 Downloads
Sugarcane related genera and species exhibit tolerance to a number of abiotic stresses. In the present study, oxidative stress response was compared between Saccharum sp and their related genera using physiological and biochemical parameters. A pot culture experiment was conducted using five clones of Erianthus arundinaceus and two commercial varieties of Saccharum hybrids. The oxidative stress was induced by external application of hydrogen peroxide (H2O2) at different concentrations (control, 300, 500, 1000 ppm) on 65 days—old plants for three consecutive days in a glass house. Adaptive response in sugarcane/wild genera was estimated in terms of chlorophyll fluorescence, chlorophyll stability index, proline, soluble protein, and reactive oxygen species scavenging enzyme activities like super oxide dismutase and peroxidase. Analysis of variance was performed and significance of each group was verified with three-way analysis of variance (chlorophyll fluorescence, peroxidase, superoxide dismutase, lipid peroxidation, protein and proline content) and two way analysis of variance (chlorophyll stability index,) followed by Tukey HSD (P < 0.05). Significant interaction between clones, H2O2 treatment and time interval was observed. Two clones of Erianthus sp (IJ76-389 and IK76-91) exhibited relatively higher tolerance for oxidative stress at 500 and 1000 ppm of H2O2 with 48 h of treatment. In Erianthus sp, the percentage increase in enzyme activity was significant; the clone IS76-139 showed maximum peroxidase (60.9% increase) and super oxide dismutase activity (189.3% increase). Cell membrane damage due to lipid peroxidation has increased with increase in concentration of H2O2. Drought tolerant sugarcane variety Co 99004 showed 50.5% reduction in lipid peroxidation when compared to the variety Co 86032. Based on physiological and biochemical response, the effective concentration to induce the oxidative stress was found to be 500 ppm at 48 h after treatment.
KeywordsSugarcane Oxidative stress ROS Chlorophyll fluorescence Chlorophyll stability index SOD Peroxidase Lipid peroxidation Protein Proline
Reactive oxygen species
Bovine serum albumin
Authors acknowledge the Indian Council of Agriculture Research (ICAR) for the funding and authors thank Director, ICAR – SBI for support and facilities.
JN, MG and RM: conducted the experiments and written the MS. RG, RA and AS analyzed the data.
Compliance with ethical standards
Conflict of interest
Authors declare no conflict of interest.
- Arisi, A. M., Cornic, G., Jouanin, L., & Foyer, C. H. (1998). Overexpression of iron superoxide dismutase in transformed poplar modifies the regulation of photosynthesis at low CO2 partial pressures or following exposure to the prooxidant herbicide methyl viologen. Plant Physiology, 117, 565–574.CrossRefPubMedPubMedCentralGoogle Scholar
- Chagas, R. M., Silveira, J. A. G., Ribeiro, R. V., Vitorello, V. A., & Carrer, H. (2008). Photochemical damage and comparative performance of superoxide dismutase and ascorbate peroxidase in sugarcane leaves exposed to paraquat-induced oxidative stress. Pesticide Biochemistry and Physiology, 90, 181–188.CrossRefGoogle Scholar
- Christy, P. M., Preetha, R. D., Vasantha, S., & Divya, D. (2013). Biochemical and molecular analysis of sugarcane genotypes response to salinity and drought. International Journal of Applied Biology and Pharmaceutical Technology, 1, 210–218.Google Scholar
- Deng, X. P., Cheng, Y. J., Wu, X. B., Kwak, S. S., Chen, W., & Eneji, A. E. (2012). Exogenous hydrogen peroxide positively influences root growth and metabolism in leaves of sweet potato seedlings. Australian Journal of Crop Science, 6, 1572–1578.Google Scholar
- Kumari, M., Dass, S., Vimala, T., & Arora, P. (2004). Physiological parameters governing drought tolerance in maize. Indian Journal of Plant Physiology, 9, 203–207.Google Scholar
- Malik, E. P., & Singh, M. B. (1980). Plant enzymology and hittoenzymology (1st ed., Vol. 286). New Delhi: Kalyani Publishers.Google Scholar
- Mohan, M. M., Narayana, S. L., & Ibrahim, S. M. (2000). Chlorophyll stability index (CSI): Its impact on salt tolerance in rice. International Rice Research Notes, 25, 38–39.Google Scholar
- Sadasivam, S., & Manickam, A. (1996). Methods in biochemistry (pp. 108–110). New Delhi: New Age International Pvt. Ltd.Google Scholar
- Upadhyaya, H., Khan, M. H., & Panda, S. K. (2007). Hydrogen peroxide induces oxidative stress in detached leaves of Oryza sativa L. General and Applied Plant Physiology, 33(1–2), 83–95.Google Scholar
- Van Toai, T. T., & Bolles, C. S. (1991). Post-anoxic injury in soybean (Glycine max) seedlings. Plant Physiology, 9, 588–592.Google Scholar
- Vijayalakshmi, D., Srividhya, S., Muthulakshmi, S., & Satishraj, R. (2014). Induction of oxidative stress by hydrogen peroxide treatment in rice genotypes to study the osmolyte accumulation pattern and antioxidant capacity. Journal of Stress Physiology & Biochemistry, 10(3), 37–46.Google Scholar
- Xu, S., Li, J., Zhang, X., Wei, H., & Cui, L. (2006). Effects of heat acclimation pre-treatment on changes of membrane lipid peroxidation, antioxidant metabolites, and ultrastructure of chloroplasts in two cool-season turf grass species under heat stress. Environmental and Experimental Botany, 56, 274–285.CrossRefGoogle Scholar