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Indian Journal of Plant Physiology

, Volume 22, Issue 3, pp 332–338 | Cite as

Study of genetic diversity of sugarcane (Saccharum) species and commercial varieties through TRAP molecular markers

  • R. B. Singh
  • Balwant Singh
  • R. K. Singh
Original Article

Abstract

Genetic variations were evaluated among the twenty-five sugarcane genotypes employing functional molecular (TRAP) markers. Genetic diversity exists among sugarcane germplasm was exploited to identify promising genotypes bearing enviable agronomic traits (sucrose content and multiple disease resistance). Genetically diversified genotype could be exploited as proven parents in sugarcane hybridization programs to establish a promising cross. TRAP markers amplify functional regions of the genome, a valuable information in relation to the variations within coding/functional regions of the plant genome. Genetic similarity (GS) among all the twenty-five genotypes was calculated in a wide range as 41–99% with an average of 70%. Thirteen unique DNA bands of Saccharum spontaneum and thirteen specific DNA bands for cultivar were produced by the used markers. Poly component analysis showed that 52.48% of the cumulative variation was explained by the all the genotypes with respect to the sucrose metabolism related genes. Sugarcane genotypes viz; CoS 96269 and CoS 8436 (GS 97.0%) and BO 91 and CoSe 95422 (GS 96%) showed highest genetic similarity. These genotypes may be recommended as proven parents for making enviable crosses in sugarcane breeding programs. Moreover, functional TRAP markers would be efficiently useful in genetic studies for sugarcane genetic improvement.

Keywords

Sugarcane genetics Genetic diversity Proven parents Poly component analysis (PCA) Genetic similarity and TRAP markers 

Notes

Acknowledgements

Authors are thankful to Ministry of Sugar and Cane Development, Govt. of Uttar Pradesh, India, and Director, Sugarcane Research Institute (U.P. Council of Sugarcane Research) to providing financial aid for conducting the research work.

Supplementary material

40502_2017_314_MOESM1_ESM.docx (13 kb)
Supplementary material 1 (DOCX 13 kb)
40502_2017_314_MOESM2_ESM.docx (12 kb)
Supplementary material 2 (DOCX 12 kb)

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Copyright information

© Indian Society for Plant Physiology 2017

Authors and Affiliations

  1. 1.Department of BiotechnologyPunjab Technical University (PTU)KapurthalaIndia
  2. 2.Swami Satyanand College of Management and TechnologyAmritsarIndia
  3. 3.Sugarcane Research Institute(U.P. Council of Sugarcane Research)ShahjahanpurIndia
  4. 4.Quality and Basic Sciences LabICAR-Indian Institute of Wheat and Barley Research (IIWBR)Agrasain Marg, KarnalIndia

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