Autoimmunity and the Oral Cavity
- 123 Downloads
Purpose of Review
This review aims to make the oral health practitioner and researcher aware of autoimmune diseases that present in the mouth and have implications for oral health. Specifically, the autoimmune pathophysiology, examples of treatments, sequelae, and impact on oral health will be discussed. The limitations of our current knowledge and understanding of these diseases will also be highlighted.
Over the past decade, it has become clear that there is a bidirectional relationship between oral health and autoimmune disease at other body sites; including diabetes and rheumatoid arthritis. Improving the oral health of patients with autoimmune diseases may reduce morbidity associated with such diseases.
Autoimmune diseases impact the oral cavity both directly and indirectly and are increasing in prevalence. As such, there is a growing need for the oral health practitioners to be aware of how autoimmune disease impacts the oral cavity. Further research is necessary to improve our understanding of the bidirectional relationship between oral health and autoimmune disease.
KeywordsOral health Oral disease Autoimmune disease
Autoimmune diseases (ADs) impact oral health, both directly and indirectly [1••]. This review will discuss ADs that present in the oral cavity as well as ADs affecting other body sites that impact on oral health. It is important for dental and medical practitioners to understand the relationships and challenges ADs present to oral health, and the bidirectional impacts between AD and oral health.
Auto-antibodies against citrullinated peptides (ACPAs) are present in 50–70% of patients [11, 12] and are associated with more severe disease. The auto-antibodies form immune complexes leading to complement activation, perpetuating inflammation and pathology. However, RA is heterogeneous; there are patients, described as ‘sero-negative’, who do not present with identifiable auto-antibodies [11, 13]. The extent to which this broad spectrum of disease presentation represents differing disease aetiologies is unconfirmed.
Periodontal disease is more prevalent in patients with rheumatoid arthritis and 80–85% of patients with RA suffer from periodontitis, compared with 40% of non-RA control patients [14••]. Patients with RA also demonstrate more alveolar bone loss compared to non-RA controls . Specifically, it has been suggested that Porphyromonas gingivalis, a key pathogen in the aetiology of periodontal disease (PD), plays a role in the development of RA . In a mouse model or RA, when periodontal disease is concurrently induced with P. gingivalis, there is an exacerbation of synovitis, compared to controls [17•]. Intriguingly, patients with RA show an elevated antibody response to P. gingivalis virulence factors [18•].
Inflammatory mediators such as interleukin 1 beta (IL-1β) and tumour necrosis factor alpha (TNF-α) have been shown to be important in RA and PD pathogenesis [19, 20], and step changes in understanding of RA pathogenesis have led to cytokine and immune cell inhibition strategies for RA treatment. There is a well-documented clinical benefit to anti-TNF-α treatments for RA patients. Further to this research has shown that patients with RA and PD receiving anti-TNF-α treatment have diminished PD. Moreover, it has been suggested that some of these cytokine targeting therapies may be less effective in patients who have uncontrolled PD. There is suggestion that anti-TNF-α and potentially other anti-pro-inflammatory cytokine therapeutics may be beneficial in the treatment of PD. 
Importantly RA is also associated with Sjögren’s Syndrome (SjS), which is discussed later in this review. RA mainly effects the joints of the hands and legs; however, temporomandibular joints can also be affected . The prevalence of temporomandibular joint dysfunction (TMD) is between 6 and 12% of the general population. A recent study of TMD in RA patients have shown around 90% of patients with RA have concurrent TMJ symptoms [23, 24]. Patients with RA and TMD show more evidence of synovitis within the TMJ when examined via MRI scanning .
The cardinal sign of SjS is destruction of exocrine glands, most notably salivary and lacrimal glands. Destruction of salivary glands causes decreased salivary flow; resulting in xerostomia. This causes an increased risk of dental caries, fungal infections, and chronic oral discomfort. As such, dentists play a key role in the diagnosis and management of this disease .
SjS pathogenesis occurs through a combination of genetic, environmental, and hormonal factors, leading to the development of inflammation and autoimmune responses to the exocrine glands . There are two variants of SjS; primary—which occurs in isolation without other systemic autoimmune diseases; and secondary which occurs following a diagnosis of systemic autoimmune disease; most commonly RA. Histologically, there is lymphocytic infiltration of the salivary and lacrimal glands. The exact role of lymphocytes in the pathogenesis of SjS is yet to be identified; however, defects in immune tolerance are an important aspect of disease development. The commonly cited auto-antibodies in SjS are antigens against La/SSB/TRIM21 and Ro60/TROVE2. As such, these act as important diagnostic markers for SjS .
There have been multiple classification criteria for SjS previously. The most recent are those developed by American College of Rheumatology (ACR) and the European League Against Rheumatism (EULAR) in 2016 . This classification allows for the simultaneous assessment of multiple systems that may be involved in SjS. As such, it has proven to be effective at both reaching a diagnosis and monitoring disease progression. It has also been beneficial in randomised controlled trials; important due to the potential development of biologics and other agents that may be used in the treatment of SjS .
A recent systematic review showed that 60.82 (95% CI 43.69 to 77.94) per 100,000 people suffer from SjS, with a strong female predilection ratio of 10.72(95% CI 7.35 to 15.62), the mean age of patients with SjS was 56.16(95% CI 52.54 to 59.78) [28, 29].
Currently, there is no cure for SjS, as such management is centred around slowing disease development and symptomatic relief. Despite biologic agents being routinely used for other ADs, they are not in routine use in SjS; however, work continues in this area .
Management from the dental team focuses on prevention and reducing oral discomfort. This includes regular oral examination, provision of high-fluoride toothpaste alongside other caries preventative methods. Saliva replacements often offer little benefit, as such conservative advice including drinking water regularly and using chewing gum may provide some relief for patients . Patients with SjS are at an increased risk of developing B cell lymphoma, which may present as swelling local to the oral cavity and salivary glands, reinforcing the need for regular dental assessment and vigilance with regard to newly arising swellings .
Chronic hyperglycaemia of diabetes results in damage to the cardiovascular system, kidneys, eyes, nerves, and patients are often immunocompromised . There are multiple oral implications of diabetes. This review focuses on type 1 diabetes due to its autoimmune pathophysiology . Diabetes treatment focuses on regulating blood glucose levels through regular testing of levels, the use of insulin, and diet control.
Type 1 diabetes arises from the autoimmune destruction of insulin-secreting pancreatic β cells causing decreased insulin production. Further to this long-standing disease often results in a total loss of insulin production. However, 10–30% of patients have no detectable auto-antibodies, and in these cases, the pathogenesis of diabetes remains unclear [34••].
Diabetes has conclusively been shown to be a risk factor for periodontal disease, and this relationship has been extensively studied and reviewed [35, 36]. Elevated HbA1c is associated with greater prevalence of disease and periodontal destruction, and glycaemic control appears to be the main predictor of attachment loss and subsequent tooth loss. Specifically, an HbA1c of greater than 7.0% appears to determine if a diabetic has and elevated PD risk [37•].
Periodontal disease negatively impacts glycaemic control and the development of nephropathy in diabetic patients [38, 39]. A recent systematic review of HbA1c levels following periodontal treatment concluded an average reduction of 0.46% of HbA1c in diabetic patients who received periodontal treatment . Thus, it is evident that there is a bidirectional relationship between PD and diabetes; the mechanisms of which are not fully understood but are thought to relate to the increased in pro-inflammatory mediators, which are involved in both diabetes and PD. 
These studies highlight the importance of ensuring that diabetes is well controlled in patients with periodontal disease, and that periodontal treatment can positively influence diabetic control.
Diabetes is a cause of xerostomia, as such, persistent dry-mouth should be investigated appropriately . Further to this, the resulting immunocompromisation from chronic hyperglycaemia predisposes diabetics to fungal infections, delayed healing after surgery, and acute dental infection that may develop quicker and take longer to resolve . Thus, it is imperative for diabetics to undergo regular dental assessment and have an appropriate prevention strategy implemented.
The ADs discussed thus far do not directly impact the oral cavity, i.e., the auto-antigens are not directed against the oral cavity. However, Systemic Lupus Erythematous (SLE) represents an interesting challenge due to its heterogeneous clinical manifestations—one of which being oral mucosal lesions. Lupus is described as a rare disease with an incidence of 4.91/100,000 people over a 3-year period; however, although incidence appears to be declining, its prevalence appears to be rising to 97.04/100,000 in 2012. Persons of Black Caribbean ethnicity have the highest incidence and prevalence . There is a higher female predilection in SLE, with an affected female to male ratio of 10–15:1 .
Lupus is a chronic AD, which affects multiple body systems including the respiratory, dermatological, musculoskeletal, renal, and cardiovascular systems. Lupus goes through periods of remission and relapse, increasing the complexity of disease management. The varied presentation of lupus is likely due to auto-antibodies being produced against nuclear and cell membrane phospholipid components. The American College of Rheumatology use the presence of auto-antibodies for double-stranded DNA and antibodies against snRNP as lupus diagnositics . These molecules are associated with apoptosis .
SLE auto-antibodies target the oral cavity directly, resulting in erythema and/or erosion with surrounding white striae, similar to that seen in oral lichen planus. Lupus lesions can affect any oral site. However, compared with lichen planus, lupus lesions more often present on the palate. Oral squamous cell carcinoma may arise within these sites, suggesting that it may be potentially malignant . As with other ADs, treatment for SLE often results in immune compromise leading to an increased risk of fungal infection.
A recent study shows that SLE has negative effects on oral health that extend to compromise the quality of life. The exact mechanisms of this are unclear .
Oral Lichen Planus
Oral lichen planus (OLP) is a chronic inflammatory condition. Both antigen-specific and non-specific mechanisms play a role in its pathophysiology . OLP manifests as a result of apoptosis of basal keratinocytes—induced by CD8+ T cells (Fig. 2B). This damage to the basal layer makes it easier for leukocytes to move into the intra-epithelial space and results in self-perpetuating inflammation . In addition, there are more degranulated mast cells present in OLP tissue compared to normal oral mucosa; degranulated mast cells release pro-inflammatory mediators and upregulate endothelial cell adhesion molecules, shown to be important in OLP pathogenesis [49, 51, 52]. Clinically, this results in painful areas of erythema, erosions, and white patches. This primarily affects the tongue and buccal mucosa but may also present as desquamative gingivitis. OLP is potentially malignant, and transformation occurs in around 1% of cases . OLP prevalence is between 1 and 2% and has a female predilection [54, 55]. Lichen planus may also affect skin and genitals, these sites are implicated in 20 and 15% of OLP cases, respectively. OLP is seen in 70–77% of cutaneous lichen planus . Whilst the exact cause of OLP remains elusive, genetic and environmental factors play a role in its pathogenesis [57•]. Environmental factors involved in OLP include trauma, dental materials, toothpastes, and systemic medications .
Oral Lichenoid Reactions (OLR) can be seen as either an exacerbation of OLP to an environmental factor or a separate disease entity, removal or modification of this factor will result in improvement or resolution of the lesions .
There is no cure for OLP as such management is focused on the symptomatic control of the disease. Treatment of the disease includes the modification of exacerbating factors, such as change of mediations implicated in OLP, removal and replacement of amalgam fillings, and modification of sharp dental cusps . Topical agents used include benzydamine or steroid preparations . In more severe cases, immune-modulating drugs such as tacrolimus, retinoids, dapsone, and mycophenolate may be necessary .
SLE has a similar clinical presentation to OLP, and graft versus host disease, which affects 40–70% of engrafted patients that may present with similar clinical and histologic features. Hence, differential diagnosis for suspected OLP lesions is important [60, 61, 62].
The vesico-bullous disorder pemphigus is an AD with the clinical presentation of blisters which evolve to painful erosions that can affect both skin and mucous membranes . Bullae formation occurs as a result of acantholysis caused by the destruction of desmosomes. Specifically, IgG auto-antibodies are directed against desmoglein 3 and desmoglein 1, resulting in loss of intra-epithelial keratinocyte adhesion  (Fig. 2C).
Oral presentation of pemphigus occurs in 50–90% of cases and is the first manifestation of the disease in around 18% of patients. Blisters and ulcerations can be present in any area of the oral mucosa, but most frequently those exposed to trauma .
Diagnosis of the disease is reached through clinical, histologic, and immunologic examination. Clinically, if pressure is applied often blisters will result, known as Nikolsky’s sign, this may indicate the presence of a blistering condition; however, concerns are raised as to its specificity and sensitivity, as well as the adverse effect of inducing blisters . The histologic examination should be undertaken, specifically, direct immunofluorescence will often identify a ‘basket weave’ appearance, showing the intra-epithelial location of auto-antibodies. Indirect immunofluorescence will detect circulating antibodies [63, 65].
Treatment of pemphigus is focused on providing symptomatic relief. Systemic corticosteroids, mycophenolate, and azathioprine are commonly used in the management of pemphigus. The biologic agent rituximab, a monoclonal anti-CD20 antibody, has been shown to achieve complete remission in 59–100% of cases; however, a relapse rate of 40–81% is reported .
Pemphigoid has a similar presentation to pemphigus, namely the formation of blisters, which often result in painful ulcers when burst [66, 67]. It can affect the skin in addition to mucous membranes. This results in a sub-epidermal blister, these are more robust than those seen in pemphigus, and Nickolsky’s sign is negative [64, 68].
The blisters result from IgG auto-antibodies directed against collagen XVII and dystonin-e; this in turn leads to destruction of hemidesmosomes responsible for the attachment of the epidermis and epithelium to basement membranes (Fig. 2D). The factors that induce pemphigoid are not understood. A genetic predisposition is important in its aetiology .
Clinical, histopathological, and immunofluorescence are employed in reaching a diagnosis of pemphigus. Histologic findings may show subepithelial bulla with some inflammation [67, 70]. Direct immunofluorescence is the gold standard for diagnosis of pemphigoid. It will typically show IgG at the basement membrane in a linear appearance, allowing differentiation between it and pemphigus ,  (Fig. 2E). Indirect immunofluorescence will show the presence of IgG antibodies .
Management of pemphigoid typically involves the use of systemic corticosteroids, and topical steroids may also be used in addition to or in isolation, if the affected area is small. Systemic treatment may include methotrexate and dapsone .
ADs can directly and indirectly affect the oral cavity and have a variety of clinical presentations. Whilst we still do not fully understand some of the mechanisms that cause ADs in the mouth or the mechanisms that cause systemic AD to impact the oral cavity, clearly, there is a bidirectional relationship. Maintaining good oral health is an important, yet often overlooked, component to the management of patients with ADs. Further studies will elucidate insights to the exact mechanisms that regulate AD in the oral cavity and the relationship between systemic AD and the oral cavity.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
Human and Animal Rights and Informed Consent
This article does not contain any studies with human or animal subjects performed by any of the authors.
Papers of particular interest, published recently, have been highlighted as: • Of importance •• Of major importance
- 1.•• Julkunen A, Heikkinen AM, Söder B, Söder P-Ö, Toppila-Salmi S, Meurman JH. Autoimmune diseases and oral health: 30-year follow-up of a Swedish cohort. Dent J. 2017;6(1):E1. https://doi.org/10.3390/dj6010001 A large study showing a relationship between autoimmune disease and oral health. CrossRefGoogle Scholar
- 12.Van Gaalen FA, Van Aken J, Huizinga TWJ, et al. Association between HLA class II genes and autoantibodies to cyclic citrullinated peptides (CCPs) influences the severity of rheumatoid arthritis. Arthritis Rheum. 2004;50(7):2113–21. https://doi.org/10.1002/art.20316.CrossRefPubMedPubMedCentralGoogle Scholar
- 14.•• Äyräväinen L, Leirisalo-Repo M, Kuuliala A, et al. Periodontitis in early and chronic rheumatoid arthritis: a prospective follow-up study in Finnish population. BMJ Open. 2017;7:e011916. https://doi.org/10.1136/bmjopen-2016-011916 Accessed august 8, 2018. A study demonstarting that periodontitis is more prevelant in patients with rheumatoid arthritis. CrossRefPubMedPubMedCentralGoogle Scholar
- 17.• Chukkapalli S, Rivera-Kweh M, Gehlot P, et al. Periodontal bacterial colonization in synovial tissues exacerbates collagen-induced arthritis in B10.RIII mice. Arthritis Res Ther. 2016;18(1):161. https://doi.org/10.1186/s13075-016-1056-4 A study showing that a key periodontal pathogen is associated with rheumatoid arthritis. CrossRefPubMedPubMedCentralGoogle Scholar
- 18.• Kharlamova N, Jiang X, Sherina N, et al. Antibodies to Porphyromonas gingivalis indicate interaction between oral infection, smoking, and risk genes in rheumatoid arthritis etiology. Arthritis Rheumatol (Hoboken, NJ). 2016;68(3):604–13. https://doi.org/10.1002/art.39491 A study showing that a key periodontal pathogen is associated with rheumatoid arthritis. CrossRefGoogle Scholar
- 31.WHO | Diabetes programme. WHO. 2018. http://www.who.int/diabetes/en/. Accessed September 4, 2018.
- 34.•• Liu Y, Bie R, Iwasaki LR, Nickel JC. Prevalence differentiations of periodontitis by diabetic status among US adults. J Diabetes. 2018;10(11):896–8. https://doi.org/10.1111/1753-0407.12781 A study showing increased prevelance in periodontal disease in patients with diabetes. CrossRefPubMedGoogle Scholar
- 37.• Faggion CM, Cullinan MP, Atieh M. An overview of systematic reviews on the effectiveness of periodontal treatment to improve glycaemic control. J Periodontal Res. 2016;51(6):716–25. https://doi.org/10.1111/jre.12358 A review of systematic reviews showing that periodontal treatment does not improve glycameic control. CrossRefPubMedGoogle Scholar
- 42.Demmer RT, Holtfreter B, Desvarieux M, Jacobs DR, Kerner W, Nauck M, et al. The influence of type 1 and type 2 diabetes on periodontal disease progression: prospective results from the Study of Health in Pomerania (SHIP). Diabetes Care. 2012;35(10):2036–42. https://doi.org/10.2337/dc11-2453.CrossRefPubMedPubMedCentralGoogle Scholar
- 68.Delgado JC, Turbay D, Yunis EJ, Yunis JJ, Morton ED, Bhol K, et al. A common major histocompatibility complex class II allele HLA-DQB1* 0301 is present in clinical variants of pemphigoid. Proc Natl Acad Sci U S A. 1996;93(16):8569–71 http://www.ncbi.nlm.nih.gov/pubmed/8710911. Accessed December 6, 2018.CrossRefGoogle Scholar
- 70.Vodegel RM, Jonkman MF, Pas HH, De Jong MCJM. U-serrated immunodeposition pattern differentiates type VII collagen targeting bullous diseases from other subepidermal bullous autoimmune diseases. Br J Dermatol. 2004;151(1):112–8. https://doi.org/10.1111/j.1365-2133.2004.06006.x.CrossRefPubMedGoogle Scholar
- 71.Sárdy M, Kostaki D, Varga R, Peris K, Ruzicka T. Comparative study of direct and indirect immunofluorescence and of bullous pemphigoid 180 and 230 enzyme-linked immunosorbent assays for diagnosis of bullous pemphigoid. J Am Acad Dermatol. 2013;69(5):748–53. https://doi.org/10.1016/j.jaad.2013.07.009.CrossRefPubMedGoogle Scholar
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.