Quantitative Biology

, Volume 2, Issue 2, pp 59–70 | Cite as

Target specificity of the CRISPR-Cas9 system

Review

Abstract

The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown great promise in correcting mutations in human genetic diseases. Off-target effects are a critical issue for all of these applications. Here we review the current status on the target specificity of the CRISPR-Cas9 system.

Keywords

CRISPR Cas9 target specificity off-targets genome engineering 

Supplementary material

40484_2014_30_MOESM1_ESM.xlsx (15 kb)
Supplementary material, approximately 15.3 KB.

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Copyright information

© Higher Education Press and Springer-Verlag GmbH 2014

Authors and Affiliations

  • Xuebing Wu
    • 1
    • 2
  • Andrea J. Kriz
    • 3
  • Phillip A. Sharp
    • 1
    • 3
  1. 1.David H. Koch Institute for Integrative Cancer ResearchMassachusetts Institute of TechnologyCambridgeUSA
  2. 2.Computational and Systems Biology Graduate ProgramMassachusetts Institute of TechnologyCambridgeUSA
  3. 3.Department of BiologyMassachusetts Institute of TechnologyCambridgeUSA

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