Advertisement

Brazilian Journal of Botany

, Volume 42, Issue 1, pp 23–28 | Cite as

Effect of lavender hydrosol on antioxidant activity and growth in Nicotiana benthamiana

  • Eun Soo Seong
  • Soo Kyung Kim
  • Jin Won Lee
  • Seung Hyuk Choi
  • Jae Geun Lee
  • Na Young Kim
  • Jong Kuk Na
  • Chang Yeon YuEmail author
Original article
  • 26 Downloads

Abstract

The present study is an investigation of lavender hydrosol as a plant growth promoter. We observed its effects on plant growth promotion, antioxidant activity, and the expression of genes related to defense in tobacco plants. Following treatment with lavender hydrosols for 2 weeks, the leaf length, leaf width, and plant height of N. benthamiana plants were 11.37 ± 0.31 cm, 11.07 ± 0.47 cm, and 14.43 ± 0.90 cm, respectively. An assay using 2,2-diphenyl-1-picrylhydrazyl (DPPH) revealed 90.08 ± 1.20% antioxidant activity, and superoxide dismutase (SOD)-like activity was 79.59 ± 3.18% in a 100% ethanol extract of plants treated with lavender hydrosol. We examined the expression patterns of genes involved in antioxidant and plant defense mechanisms at the transcriptional level in N. benthamiana cells; such genes include those that code for superoxide dismutase (SOD), ascorbate peroxidase (APX), phenylalanine ammonia lyase (PAL), nitrate reductase (NR), and nitric oxide-associated 1 (NOA1). All the genes examined were expressed more in the plants treated with lavender hydrosol than in the untreated controls. The results suggest that lavender hydrosol promotes antioxidant activity in plant cells by activating antioxidant and plant defense mechanisms, ultimately promoting plant growth.

Keywords

Defense Plant growth promotion Transcriptional level 

Notes

Acknowledgements

This work was supported by the Bioherb Research Institute, Kangwon National University, Republic of Korea.

Author contributions

ESS, SKK, JWL, and SHC performed experiment design and writing of manuscript. CYY supervised the experiment. JGL, NYK, and JKN performed editing of manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Adsersen A, Gauguin B, Gudiksen L, Jager AK (2006) Screening of plants used in Danish folk medicine to treat memory dysfunction for acetylcholinesterase inhibitory activity. J Ethnopharma 104:418–422CrossRefGoogle Scholar
  2. Ann SW, Kim YC, Hwang IS, Cho JK, Kim MS, Lee JK, Eum WY (2010) Effect of seafood amino acid fertilizer and Korean effective microorganisms on the fruit quality of Fuji apple. J Environ Sci 10:1293–1299Google Scholar
  3. Asada K (1999) The water–water cycle in chloroplasts: scavenging of active oxygen and dissipation of excess photons. Ann Rev Plant Physiol Plant Mol Biol 50:601–639CrossRefGoogle Scholar
  4. Badawi GH, Kawano N, Yamauchi Y, Shimada E, Sasaki R, Kubo A, Tanaka K (2004) Over-expression of ascorbate peroxidase in tobacco chloroplasts enhances the tolerance to salt stress and water deficit. Physiol Plant 121:231–238CrossRefGoogle Scholar
  5. Bright J, Desikan R, Hancock JT, Weir IS, Neill SJ (2006) ABA induced NO generation and stomatal closure in Arabidopsis are dependent on H2O2 synthesis. Plant J 45:113–122CrossRefGoogle Scholar
  6. Catty S (2001) Hydrosols: the next aromatherapy. Healing Art Press, Rochester, pp 9–10, 12, 28Google Scholar
  7. Cavin A, Hostettmann K, Dyatmyko W, Potterat O (1998) Antioxidant and lipophilic constituents of Tinospora crispa. Planta Med 64:393–396CrossRefGoogle Scholar
  8. Cheong YH, Han MJ, Sung SJ, Seo DC, Kang JG, Sohn BK, Heo JS, Cho JS (2009) Effects of selenium supplement on germination, sprout growth and selenium uptake in four vegetables. Korean J Environ Agric 28:179–185CrossRefGoogle Scholar
  9. Crawford NM, Galli M, Tischner R, Heimer YM, Okamoto M, Mack A (2006) Plant nitric oxide synthase: back to square one. Trends Plant Sci 11:526–527CrossRefGoogle Scholar
  10. Dapkevicius A, Venskutonis R, van Beek TA, Linssen JPH (1998) Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. J Sci Food Agric 77:140–146CrossRefGoogle Scholar
  11. Desikan R, Griffiths R, Hancock J, Neill S (2002) A new role for an old enzyme: nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsis thaliana. Proceed Natl Acad Sci USA 99:16314–16318CrossRefGoogle Scholar
  12. Dixon RA, Paiva NL (1995) Stress-induced phenylpropanoid metabolism. Plant Cell 7:1085–1097CrossRefGoogle Scholar
  13. Han SH, Kim DH, Kim HS, Cho DH, Yoo SG, Kim PK (2009) Effects of SCB liquid fertilizer on physiological activity of seed and seedling of Pinus densiflora Sieb. et Zucc and Maackia amurensis Rupr. et Maxim. Korean Soc Forest Sci Acad Con pp 236–237Google Scholar
  14. Hritcu L, Cioanca O, Hancianu M (2012) Effects of lavender oil inhalation on improving scopolamine-induced spatial memory impairment in laboratory rats. Phytomed 19:529–534CrossRefGoogle Scholar
  15. Jeon DH, Moon JY, Hyun HB, Cho SK (2013) Composition analysis and antioxidant activities of the essential oil and the hydrosol extracted from Rosmarinus officinalis L. and Lavandula angustifolia Mill. produced in Jeju. J Appl Biol Chem 56:141–146CrossRefGoogle Scholar
  16. Kato H, Asai S, Yamamoto-Katou A, Yoshioka H, Doke N, Kawakita K (2008) Involvement of NbNOA1 in NO production and defense responses in INF1-treated Nicotiana benthamiana. J Gene Plant Pathol 74:15–23CrossRefGoogle Scholar
  17. Kim JH, Kim MJ, Choi SK, Bae SH, An SK, Yoon YM (2011) Antioxidant and antimicrobial effects of lemon and eucalyptus essential oils. J Soc Cosmet Sci 37:303–308Google Scholar
  18. Kunicka-Styczynska A, Smigielski K, Prusinowska R, Rajkowska K, Kusmider B, Sikora M (2014) Preservative activity of lavender hydrosols in moisturizing body gels. Lett Appl Microbiol 60:27–32CrossRefGoogle Scholar
  19. Lee CK, Cho KC, Lee JH, Cho JY, Seo BS, Yang WM (2005) Effects of selenium supplying methods on the growth and Se uptake of hydroponically grown tomato plants. J Bio Environ Control 14:284–288Google Scholar
  20. Liu R, Xu S, Li J, Hu Y, Lin Z (2006) Expression profile of a PAL gene from Astragalus membranaceus var. mongholicus and its crucial role in flux into flavonoids biosynthesis. Plant Cell Rep 25:705–710CrossRefGoogle Scholar
  21. Marklund S, Marklund G (1974) Involvement of superoxide anion radical in autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Euro J Biochem 47:469–474CrossRefGoogle Scholar
  22. Michalak A (2006) Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish J Environ Stud 15:523–530Google Scholar
  23. Monica H, Oana C, Marius M, Lucian H (2013) Neuroprotective effects of inhaled lavender oil on scopolamine-induced dementia via antioxidative activities in rats. Phytomed 20:446–452CrossRefGoogle Scholar
  24. Parejo I, Viladomat F, Bastida J, Rosas R, Flerlage N, Burillo J, Codina C (2002) Comparison between the radical scavenging activity and antioxidant activity of six distilled and nondistilled mediterranean herbs and aromatic plants. J Agric Food Chem 50:6882–6890CrossRefGoogle Scholar
  25. Romero I, Sanchez-Ballesta MT, Maldonado R, Isabel Escribano M, Merodio C (2008) Anthocyanin, antioxidant activity and stress-induced gene expression in high CO2-treated table grapes stored at low temperature. J Plant Physiol 165:522–530CrossRefGoogle Scholar
  26. Rose J (1999) 375 Essential oils and hydrosols. Frog, Ltd, Berkeley, pp 163–164Google Scholar
  27. Sait C, Ahmet O (2002) Effects of intraperitoneally administered lipoic acid, vitamin E, and linalool on the level of total lipid and fatty acids in guinea pig brain with oxidative stress induced by H2O2. J Biochem Mol Biol 35:547–552Google Scholar
  28. Shin YH, Kim HJ, Lee JY, Cho YJ, An BJ (2012) Major compound analysis and assessment of natural essential oil on anti-oxidative and anti-microbial effects. J Life Sci 20:1344–1351CrossRefGoogle Scholar
  29. Tannous P, Juliani R, Wang M, Simon S (2004) Water balance in hydrosol production via steam distillation: case study using lavandin (Lavandula x intermadia). New Use Agriculture and natural Plant Products and ASNAPP Program, February 09, New Jersey, USAGoogle Scholar
  30. Zhao MG, Chen L, Zhang LL, Zhang WH (2009) Nitric reductase dependent nitric oxide production is involved in cold acclimation and freezing tolerance in Arabidopsis. Plant Physiol 151:755–767CrossRefGoogle Scholar

Copyright information

© Botanical Society of Sao Paulo 2019

Authors and Affiliations

  • Eun Soo Seong
    • 1
  • Soo Kyung Kim
    • 1
  • Jin Won Lee
    • 1
  • Seung Hyuk Choi
    • 2
  • Jae Geun Lee
    • 3
  • Na Young Kim
    • 4
  • Jong Kuk Na
    • 5
  • Chang Yeon Yu
    • 2
    Email author
  1. 1.Department of Medicinal PlantSuwon Women’s UniversitySuwonSouth Korea
  2. 2.Department of Bio-Resource SciencesKangwon National UniversityChuncheonSouth Korea
  3. 3.Hwajin CosmeticsHongcheonSouth Korea
  4. 4.Hotel Culinary Arts, Songho UniversityHoengseongSouth Korea
  5. 5.Department of Controlled AgricultureKangwon National UniversityChuncheonSouth Korea

Personalised recommendations