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Triploid frequency of sexual hybridization and pollen and ovary development in mandarins

  • Shirley Nascimento Costa
  • Priscila Andressa Cortez
  • Lucas Aragão da Hora Almeida
  • Fabiano Machado Martins
  • Walter dos Santos Soares Filho
  • Mauricio Antônio Coelho Filho
  • Abelmon da Silva GesteiraEmail author
Original article
  • 37 Downloads

Abstract

This work aimed to ascertain the triploid frequency of sexual hybridization and the pollen and ovary development in Citrus. The ‘Fortune’ mandarin (Citrus clementina Hort. ex Tan. × C. tangerina Hort. ex Tan.) accession was selected as female parent for this study based on its reproductive characteristics, mainly the monoembryony and self-incompatibility. The male parents used were the ‘Cravo’ (C. reticulata, Blanco) and ‘Dancy’ (C. reticulata) mandarins. The pollinations were always manually performed in the morning, and the pollinated flowers were identified for control. During the flowering period, flower samples at different stages of development of the ‘Fortune’ variety were collected for histological analysis. Microspore development occurred regularly in the 4–5 mm (length) phase of the floral buds, and a bicellular microgametophyte was formed in the floral buds with 5–6 mm in length. The formation of the female gametophyte occurred later, in flower buds with approximately 10 mm in length. The hybridizations resulted in 19 triploid plants: five when the ‘Cravo’ mandarin was used as the male parent and 14 when ‘Dancy’ mandarin was used as the male parent, evidencing the importance of the male genitor also in this process.

Keywords

Clementine × C. tangerine Flower Unreduced gametes 

Notes

Acknowledgements

We thank Liziane Marques dos Santos, Maria Aparecida dos Santos de Jesus and Nayara de Almeida Santos for their kind technical assistance; the Electron Microscopy Center of Santa Cruz State University (UESC), Ilhéus, BA, for providing the facilities for microscopic analyses; the National Council for Scientific and Technological Development (CNPq) for the financial support (Grants 301356/2012-2 and 472733/2013-3); and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the scholarship to the first author.

Authors contribution

SNC was involved in conception and design of experiment, acquisition of data, analysis and interpretation of data, and writing and revision of the manuscript. PAC acquired, analyzed and interpreted the data and wrote and revised the manuscript. LAHA was involved in conception and design of the experiment. FMM analyzed and interpreted the data and wrote and revised the manuscript. WSSF was involved in conception and design of the experiment. MACF was involved in conception and design of the experiment. ASG was involved in conception and design of the experiment, writing and revision of the manuscript.

References

  1. Abouzari A, Mahdi MN (2016) The investigation of Citrus fruit quality. Popular characteristic and breeding. Acta Univ Agric Silvic Mendel Brun 64:725–740.  https://doi.org/10.11118/actaun201664030725 CrossRefGoogle Scholar
  2. Aleza P, Juárez J, Ollitrault P, Navarro L (2009) Production of tetraploid plants of non apomictic Citrus genotypes. Plant Cell Rep 28:1837–1846.  https://doi.org/10.1007/s00299-009-0783-2 CrossRefGoogle Scholar
  3. Aleza P, Juárez J, Cuenca J, Ollitrault P, Navarro L (2010) Recovery of Citrus triploid hybrids by embryo rescue and flow cytometry from 2x × 2x sexual hybridisation and its application to extensive breeding programs. Plant Cell Rep 29:1023–1103.  https://doi.org/10.1007/s00299-010-0888-7 CrossRefGoogle Scholar
  4. Aleza P, Cuenca J, Hernández M, Juárez J, Navarro L, Ollitrault P (2015) Genetic mapping of centromeres in the nine Citrus clementina chromosomes using half-tetrad analysis and recombination patterns in unreduced and haploid gametes. BMC Plant Biol 15:80–93.  https://doi.org/10.1186/s12870-015-0464-y CrossRefGoogle Scholar
  5. Bono R, Soler J, Buj A, Villalba D,Salvia J, Bellver R, Mares M, Méndez JV, Gavilá L (2006) El problema de la presencia de semillas en los frutos cítricos. Conselleria de Agricultura Pesca y Alimentación, Ficha Técnica serie citricultura, 5Google Scholar
  6. Chen C, Lyon MT, O’Malley D, Federici C, Gmitter J, Gosser JW, Chaparro JX, Rosse ML, Gmitter FR Jr (2008) Origen and frequency of 2n gametes in Citrus sinensis × Poncirius trifoliata and their reciprocal crosses. Plant Sci 174:1–8.  https://doi.org/10.1016/j.tig.2007.08.005 CrossRefGoogle Scholar
  7. Cruz CD (2006) Programa Genes: Biometria, 1st edn. Editora UFV, Viçosa (MG)Google Scholar
  8. D’Erfurth I, Jolivet S, Froger N, Catrice O, Novatchkova M, Simon M, Jenczewski E, Mercier R (2008) Mutations in AtPS1 (Arabidopsis thaliana parallel spindle 1) lead to the production of diploid pollen grains. PLoS Genet 4:e1000274.  https://doi.org/10.1371/journal.pgen.1000274 CrossRefGoogle Scholar
  9. Dolezel J, Greilhuber J, Suda J (2007) Estimation of nuclear DNA content in plants using flow cytometry. Nat Protoc 2:2233–2244.  https://doi.org/10.1038/nprot.2007.310 CrossRefGoogle Scholar
  10. Esen A, Soost RK (1971) Unexpected triploids in citrus: their origin, identification and possible use. J Hered 62:329–333.  https://doi.org/10.1093/oxfordjournals.jhered.a108186 CrossRefGoogle Scholar
  11. Food and Agriculture Organization of the United Nations. Food and agriculture (2017). FAO http://www.fao.org/faostat/en/#home. Acessed 27 July 2017
  12. Gerlach G (1969) Botanische mikrotechnik: eine einfuhrung, 3rd edn. Georg Thieme Verlag, StuttgartGoogle Scholar
  13. Geraci G, Esen A, Soost RK (1975) Triploid progenies from 2x x 2x crosses of Citrus cultivars. J Hered 66:177–178.  https://doi.org/10.1093/oxfordjournals.jhered.a108607 CrossRefGoogle Scholar
  14. Guerra M (2009) Chromosomal variability and the origin of Citrus species. In: Mahone CL, Springer DA (eds) Genetic diversity. Nova Science Publishers Inc, New York, pp 51–68Google Scholar
  15. Hodgson RW (1967) Horticultural varieties of Citrus. In: Reuther W, Webber HJ, Batchelor LD (eds) The Citrus industry. University of California Press, California, pp 432–459Google Scholar
  16. Iglesias DJ, Cercós M, Colmenero-Flores JM, Naranjo Ríos G, Carrera E, Ruiz-Rivero O, Lliso-Morillon LR, Tadeo FR, Talon M (2007) Physiology of Citrus fruiting. Braz J Plant Physiol 19:333–362.  https://doi.org/10.1590/S1677-04202007000400006 CrossRefGoogle Scholar
  17. Johansen DA (1940) Plant microtechnique, 1st edn. McGraw-Hill Book Company, New YorkGoogle Scholar
  18. Kimata Y, Higaki T, Kawashima T, Kurihara D, Sato Y, Yamada T, Seiichiro H, Frederic B, Tetsuya H, Ueda M (2016) Cytoskeleton dynamics control the first asymmetric cell division in Arabidopsis zygote. Proc Natl Acad Sci USA.  https://doi.org/10.1073/pnas.1613979113 Google Scholar
  19. Lloyd G, Mccown B (1981) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Int Plant Propag Soc 30:421–427Google Scholar
  20. Mason AS, Pires JC (2015) Unreduced gametes: meiotic mishap or evolutionary mechanism? Trends Genet 31:5–10.  https://doi.org/10.1016/j.pmr.2014.09.011 CrossRefGoogle Scholar
  21. Ollitrault P, Dambier D, Luro F, Froelicher Y (2008) Ploidy manipulation for breeding seedless triploid Citrus. Plant Breed Rev 30:323–354.  https://doi.org/10.1002/9780470380130.ch7 CrossRefGoogle Scholar
  22. Otto SP (2007) The evolutionary consequences of polyploidy. Cell 131:452–462.  https://doi.org/10.1016/j.cell.2007.10.022 CrossRefGoogle Scholar
  23. Pécrix Y, Rallo G, Folzer H, Cigna M, Gudin S, Manuel Le Bris M (2011) Polyploidization mechanisms: temperature environment can induce diploid gamete formation in Rosa sp. J Exp Bot 62:3587–3597.  https://doi.org/10.1093/jxb/ers387 CrossRefGoogle Scholar
  24. Soost RK, Cameron JW (1975) Advances in fruit breeding. In: Jannick J, Moore JN (eds) Citrus. Purdue University Press, West Lafayette, pp 507–540Google Scholar
  25. Storme N, Geelen D (2011) The Arabidopsis mutant jason produces unreduced first division restitution male gametes through a parallel/fused spindle mechanism in meiosis II. Plant Physiol 155:1403–1415.  https://doi.org/10.1104/pp.110.170415 CrossRefGoogle Scholar
  26. Storme N, Geelen D (2013) Sexual polyploidization in plants—cytological mechanisms and molecular regulation. New Phytol 198:670–684.  https://doi.org/10.1111/nph.12184 CrossRefGoogle Scholar
  27. Van Genuchten MT (1980) A closed form equation for predicting the hydraulic conductivity of unsaturated soils. Soil Sci Soc Am J 44:892–898.  https://doi.org/10.2136/sssaj1980.03615995004400050002x CrossRefGoogle Scholar
  28. Velázquez TLC, Hernández ARA, Figueroa-Castro DM, Villanueva AC (2016) Anatomy of the reproductive structures of Stenanona flagelliflora (Annonaceae). Brazi J Bot 39:679–687.  https://doi.org/10.1007/s40415-016-0252-4 CrossRefGoogle Scholar
  29. Wang X, Cheng ZM, Zhi S, Xu F (2016) Breeding triploid plants: a review. Czech J Genet Plant Breed 52:41–54.  https://doi.org/10.17221/151/2015-CJGPB CrossRefGoogle Scholar

Copyright information

© Botanical Society of Sao Paulo 2019

Authors and Affiliations

  • Shirley Nascimento Costa
    • 1
    • 2
  • Priscila Andressa Cortez
    • 1
  • Lucas Aragão da Hora Almeida
    • 1
  • Fabiano Machado Martins
    • 3
  • Walter dos Santos Soares Filho
    • 4
  • Mauricio Antônio Coelho Filho
    • 4
  • Abelmon da Silva Gesteira
    • 1
    • 4
    Email author
  1. 1.Universidade Estadual de Santa CruzIlhéusBrazil
  2. 2.Faculdade Santo AntônioAlagoinhasBrazil
  3. 3.Universidade Federal do Recôncavo da BahiaCruz das AlmasBrazil
  4. 4.Embrapa Mandioca e FruticulturaCruz das AlmasBrazil

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