Genetic characterization of Allium stipitatum accessions: an economically wild edible Allium species with unique flavor
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We evaluated the genetic diversity of Iranian Mooseer (Allium stipitatum Regel. syn. A. hirtifolium Boiss.) using morphological traits and AFLP markers and determined the phylogenetic relationships and the position of Iranian Mooseer among domesticated and wild edible alliums using internal transcribed spacer (ITS) sequence data. Four EcoRI and MseI AFLP primer combinations rendered produced 376 markers, 204 being polymorphic (53.28% polymorphism). AFLP data separated clearly the Kazeroun accession, originating from the warmer Southern region of Iran, from the other 20 accessions. According to the morphological evaluation data, Khansar, Kouhrang and Daran accessions showed the highest means for bulb weight. Cluster analysis based on morphological traits divided the Mooseer germplasms into three main groups. The ITS results indicated that Iranian Mooseer clustered with other A. stipitatum accessions, but with small differences. They were phylogenetically close to Iranian wild edible alliums and clearly differentiated from other domesticated alliums. Separation of three Iranian Mooseers from A. stipitatum samples originated from other countries in two distinct groups according to their ITS sequence shows that probably these two groups have divergent evolutionary paths.
KeywordsMooseer Morphology AFLP ITS Phylogeny Germplasm
Authors would like to thank University of Tehran and the Flanders Research Institute for Agriculture, Fisheries and Food (ILVO, Merelbeke, Belgium) for supporting this research.
R. Ebrahimi collected the samples. She also designed and performed the experiments and wrote the manuscript. M.R. Hassandokht and Z. Zamani supervised the project and verified the findings of this work. They also helped in interpretation of results. I. Roldan-Ruiz supervised the molecular analysis and helped in structuring the manuscript. H. Muylle contributed to molecular analysis, data analysis and interpretation of the results. S. Van Glabeke helped in technical work and data analysis. E. Van Bockstaele provided critical feedback and helped shape research, analysis and manuscript. A. Kashi helped in morphological analysis.
- Blattner FR (1999) Direct amplification of the entire ITS region from poorly preserved plant material using recombinant PCR. Bio Techniques 27:1180–1186Google Scholar
- Doyle JJ, Doyle GL (1990) Isolation of DNA from fresh plant tissue. Focus 12:13–15Google Scholar
- Ebrahimi R, Zamani Z, Kashi A, Jabbari A (2008) Comparison of fatty acids, mineral elements of 17 Iranian shallot landraces (Allium hirtifolium Boiss.). J Food Technol 5:61–68Google Scholar
- Ebrahimi R, Zamani Z, Hassandokht MR, Kashi A (2011) Persian shallot (Allium hirtifolium Boiss.): an endangered wild plant. In: Davis RE (ed) Wild plants: identification, uses and conservation. NOVA Press, New York, pp 289–304Google Scholar
- Fritsch RM, Abbasi M, Keusgen M (2006) Useful wild Allium species in Northern Iran. Rostaniha 7:189–206Google Scholar
- Hanelt P, Schultze-Motel J, Fritsch R, Kruse J, Maab HI, Ohle H, Pistrick K (1992) Infrageneric grouping of Allium, the Gatersleben approach. In: Hanelt P, Hammer K, Knüpffer H (eds) The genus Allium taxonomic problems and genetic resources. Proceedings of an international symposium, Gatersleben, 11–13 June IPK, Gatersleben, Germany, pp 107–123Google Scholar
- IBM Corp. Released 2010. IBM SPSS Statistics for Windows, Version 19.0. IBM Corp, ArmonkGoogle Scholar
- Khezri S (2003) Encyclopedia of medicinal plants. Rostamkhani Publication, Tehran, p 568Google Scholar
- Mantel NA (1967) The detection of disease clustering and a generalized approach. Cancer Res 27:209–227Google Scholar
- StatSoft, Inc (2012) STATISTICA (data analysis software system), version 11. www.statsoft.com