Brazilian Journal of Botany

, Volume 38, Issue 2, pp 265–272 | Cite as

Effects of MeJA and SA elicitation on secondary metabolic activity, antioxidant content and callogenesis in Phyllanthus pulcher

  • Mahmoud Danaee
  • Reza Farzinebrahimi
  • Mihdzar Abdul Kadir
  • Uma Rani Sinniah
  • Rosfarizan Mohamad
  • Rosna Mat Taha
Article
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Abstract

To develop in vitro callus induction of Phyllanthus pulcher and investigate secondary metabolite activities, methyl jasmonate (MeJA) and salicylic acid (SA) as two abiotic elicitors were evaluated. Growth and secondary metabolite production of callus from P. pulcher grown in Murashige and Skoog medium, supplemented with 1 mg L−1 2, 4-dinitrophenylhydrazine (2, 4-D) and SA with a lower concentration (≤5 mg L−1) showed a significant effect on callus fresh weight, while the dry weight was not affected significantly by higher concentrations. However, in secondary metabolite content, including total flavonoid and phenolic contents and antioxidant activities, significant differences among different SA concentrations were observed. The results revealed that the high concentration of MeJA (>10 mM) inhibits the callus growth, and 1 mM of MeJA resulted in the highest yield for total flavonoid and phenolic contents and antioxidant activity. A very strong positive relationship (0.969) among TFC, TPC, and antioxidant activity was revealed.

Keywords

Callus Elicitor Methyl jasmonate Phyllanthus pulcher Salicylic acid 

Abbreviations

2,4-D

2,4-Dichlorophenoxy acetic acid

AA

Antioxidant activity

ANCOVA

Analysis of covariance

ANOVA

Analysis of variance

DMRT

Duncan’s multiple range test

DW

Dry weight

FW

Fresh weight

MeJA

Methyl jasmonate

MS

Murashige and Skoog (1962) medium

PGR

Plant growth regulators

RCBD

Randomized complete block design

SA

Salicylic acid

TFC

Total flavonoid content

TPC

Total phenolic content

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Notes

Acknowledgments

This study was supported by University Putra Malaysia. We also thank the University of Malaya for supporting and helping to publish this research.

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Copyright information

© Botanical Society of Sao Paulo 2015

Authors and Affiliations

  • Mahmoud Danaee
    • 1
    • 6
  • Reza Farzinebrahimi
    • 5
  • Mihdzar Abdul Kadir
    • 2
  • Uma Rani Sinniah
    • 3
  • Rosfarizan Mohamad
    • 4
  • Rosna Mat Taha
    • 5
  1. 1.Faculty of AgricultureIslamic Azad University, Roudehen BranchRudehen, TehranIran
  2. 2.Department of Agricultural Technology, Faculty of AgricultureUniversity Putra MalaysiaSerdangMalaysia
  3. 3.Department of Crop Science, Faculty of AgricultureUniversity Putra MalaysiaSerdangMalaysia
  4. 4.Department of Bioprocess, Faculty of BiotechnologyUniversity Putra MalaysiaSerdangMalaysia
  5. 5.Institute of Biological Sciences, Faculty of ScienceUniversity of MalayaKuala LumpurMalaysia
  6. 6.UMCAS, Faculty of ScienceUniversity of MalayaKuala LumpurMalaysia

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