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European Archives of Paediatric Dentistry

, Volume 19, Issue 1, pp 1–22 | Cite as

Biodentine™ material characteristics and clinical applications: a 3 year literature review and update

  • S. RajasekharanEmail author
  • L. C. Martens
  • R. G. E. C. Cauwels
  • R. P. Anthonappa
Systematic Review

Abstract

Introduction

Biodentine™ has frequently been acknowledged in the literature as a promising material and serves as an important representative of tricalcium silicate based cements used in dentistry.

Aim

To provide an update on the physical and biological properties of Biodentine™ and to compare these properties with those of other tricalcium silicate cements namely, different variants of mineral trioxide aggregate (MTA) such as ProRoot MTA, MTA Angelus, Micro Mega MTA (MM-MTA), Retro MTA, Ortho MTA, MTA Plus, GCMTA, MTA HP and calcium enriched mixture (CEM), Endosequence and Bioaggregate™.

Study design

A comprehensive literature search for publications from November 20, 2013 to November 20, 2016 was performed by two independent reviewers on Medline (PubMed), Embase, Web of Science, CENTRAL (Cochrane), SIGLE, SciELO, Scopus, Lilacs and clinicaltrials.gov. Electronic and hand search was carried out to identify randomised control trials (RCTs), case control studies, case series, case reports, as well as in vitro and animal studies published in the English language.

Conclusions

The enhanced physical and biologic properties of Biodentine™ could be attributed to the presence of finer particle size, use of zirconium oxide as radiopacifier, purity of tricalcium silicate, absence of dicalcium silicate, and the addition of calcium chloride and hydrosoluble polymer. Furthermore, as Biodentine™ overcomes the major drawbacks of MTA it has great potential to revolutionise the different treatment modalities in paediatric dentistry and endodontics especially after traumatic injuries. Nevertheless, high quality long-term clinical studies are required to facilitate definitive conclusions.

Keywords

Biodentine™ Tricalcium silicate 

Notes

Funding

The authors declare that no funding was received for this review.

Compliance with ethical standards

Conflict of interest

The research group received material support from Septodont, Paris, France for educational programmes intended for general dentists.

References

  1. Aggarwal V, Singla M, Yadav S, Yadav H, Ragini H. Marginal adaptation evaluation of Biodentine and MTA plus in “Open Sandwich” class II restorations. J Esthet Restor Dent. 2015;27:167–75.PubMedCrossRefGoogle Scholar
  2. Agrafioti A, Tzimpoulas N, Chatzitheodoridis E, Kontakiotis EG. Comparative evaluation of sealing ability and microstructure of MTA and Biodentine after exposure to different environments. Clin Oral Investig. 2015;20:1535–40.PubMedCrossRefGoogle Scholar
  3. Aldakak MM, Capar ID, Rekab MS, Abboud S. Single-visit pulp revascularization of a nonvital immature permanent tooth using Biodentine. Iran Endod J. 2016;11:246–9.PubMedPubMedCentralGoogle Scholar
  4. Alhodiry W, Lyons MF, Chadwick RG. Effect of saliva and blood contamination on the bi-axial flexural strength and setting time of two calcium-silicate based cements: Portland cement and Biodentine. Eur J Prosthodont Restor Dent. 2014;22:20–3.PubMedGoogle Scholar
  5. Alsubait SA, Hashem Q, Alhargan N, Almohimeed K, Alkahtani A. Comparative evaluation of push-out bond strength of ProRoot MTA, bioaggregate and Biodentine. J Contemp Dent Pract. 2014;15:336–40.PubMedCrossRefGoogle Scholar
  6. Altunsoy M, Tanriver M, Ok E, Kucukyilmaz E. Shear bond strength of a self-adhering flowable composite and a flowable base composite to mineral trioxide aggregate, calcium-enriched mixture cement, and Biodentine. J Endod. 2015;41:1691–5.PubMedCrossRefGoogle Scholar
  7. Atmeh AR, Chong EZ, Richard G, et al. Calcium silicate cement-induced remineralisation of totally demineralised dentine in comparison with glass ionomer cement: tetracycline labelling and two-photon fluorescence microscopy. J Microsc. 2015;257:151–60.PubMedCrossRefGoogle Scholar
  8. Attik GN, Villat C, Hallay F, et al. In vitro biocompatibility of a dentine substitute cement on human MG63 osteoblasts cells: Biodentine versus MTA((R)). Int Endod J. 2014;47:1133–41.PubMedCrossRefGoogle Scholar
  9. Bajwa NK, Jingarwar MM, Pathak A. Single visit apexification procedure of a traumatically injured tooth with a novel bioinductive material (Biodentine). Int J Clin Pediatr Dent. 2015;8:58–61.PubMedPubMedCentralCrossRefGoogle Scholar
  10. Bani M, Sungurtekin-Ekci E, Odabas ME. Efficacy of Biodentine as an apical plug in nonvital permanent teeth with open apices: an in vitro study. Biomed Res Int. 2015;2015(2015):4.Google Scholar
  11. Baranwal AK. Management of external invasive cervical resorption of tooth with Biodentine: a case report. J Conserv Dent. 2016;19:296–9.PubMedPubMedCentralCrossRefGoogle Scholar
  12. Bayram E, Bayram HM. Fracture resistance of immature teeth filled with mineral trioxide aggregate, bioaggregate, and Biodentine. Eur J Dent. 2016;10:220–4.PubMedPubMedCentralCrossRefGoogle Scholar
  13. Beatty H, Svec T. Quantifying coronal tooth discoloration caused by Biodentine and endosequence root repair material. J Endod. 2015;41:2036–9.PubMedCrossRefGoogle Scholar
  14. Bhat SS, Hegde SK, Adhikari F, Bhat VS. Direct pulp capping in an immature incisor using a new bioactive material. Contemp Clin Dent. 2014;5:393–6.PubMedPubMedCentralCrossRefGoogle Scholar
  15. Bhavana V, Chaitanya KP, Gandi P, et al. Evaluation of antibacterial and antifungal activity of new calcium-based cement (Biodentine) compared to MTA and glass ionomer cement. J Conserv Dent. 2015;18:44–6.PubMedPubMedCentralCrossRefGoogle Scholar
  16. Bolhari B, Ashofteh Yazdi K, Sharifi F, Pirmoazen S. Comparative scanning electron microscopic study of the marginal adaptation of four root-end filling materials in presence and absence of blood. J Dent (Tehran). 2015;12:226–34.Google Scholar
  17. Borkar S, De Noronha De Ataide I. Management of a massive resorptive lesion with multiple perforations in a molar: case report. J Endod. 2015;41:753–8.PubMedCrossRefGoogle Scholar
  18. Borkar SA, Ataide I. Biodentine pulpotomy several days after pulp exposure: four case reports. J Conserv Dent. 2015;18:73–8.PubMedPubMedCentralCrossRefGoogle Scholar
  19. Bortoluzzi EA, Niu LN, Palani CD, et al. Cytotoxicity and osteogenic potential of silicate calcium cements as potential protective materials for pulpal revascularization. Dent Mater. 2015;31:1510–22.PubMedPubMedCentralCrossRefGoogle Scholar
  20. Butt N, Talwar S, Chaudhry S, et al. Comparison of physical and mechanical properties of mineral trioxide aggregate and Biodentine. Indian J Dent Res. 2014;25:692–7.PubMedCrossRefGoogle Scholar
  21. Camilleri J. Hydration characteristics of Biodentine and theracal used as pulp capping materials. Dent Mater. 2014;30:709–15.PubMedCrossRefGoogle Scholar
  22. Camilleri J. Staining potential of Neo MTA plus, MTA plus, and Biodentine used for pulpotomy procedures. J Endod. 2015;41:1139–45.PubMedCrossRefGoogle Scholar
  23. Camilleri J, Sorrentino F, Damidot D. Investigation of the hydration and bioactivity of radiopacified tricalcium silicate cement. Biodentine and MTA angelus. Dent Mater. 2013;29:580–93.PubMedCrossRefGoogle Scholar
  24. Camilleri J, Grech L, Galea K, et al. Porosity and root dentine to material interface assessment of calcium silicate-based root-end filling materials. Clin Oral Investig. 2014a;18:1437–46.PubMedCrossRefGoogle Scholar
  25. Camilleri J, Laurent P, About I. Hydration of Biodentine, Theracal LC, and a prototype tricalcium silicate-based dentin replacement material after pulp capping in entire tooth cultures. J Endod. 2014b;40:1846–54.PubMedCrossRefGoogle Scholar
  26. Cantekin K, Avci S. Evaluation of shear bond strength of two resin-based composites and glass ionomer cement to pure tricalcium silicate-based cement (Biodentine(R)). J Appl Oral Sci. 2014;22:302–6.PubMedPubMedCentralCrossRefGoogle Scholar
  27. Caron G, Azerad J, Faure MO, Machtou P, Boucher Y. Use of a new retrograde filling material (Biodentine) for endodontic surgery: two case reports. Int J Oral Sci. 2014;6:250–3.PubMedPubMedCentralCrossRefGoogle Scholar
  28. Caronna V, Himel V, Yu Q, Zhang JF, Sabey K. Comparison of the surface hardness among 3 materials used in an experimental apexification model under moist and dry environments. J Endod. 2014;40:986–9.PubMedCrossRefGoogle Scholar
  29. Cechella BC, De Almeida J, Felippe MC, et al. Influence of phosphate buffered saline on the bond strength of endodontic cement to dentin. Braz J Oral Sci. 2015;14:126–9.CrossRefGoogle Scholar
  30. Ceci M, Beltrami R, Chiesa M, Colombo M, Poggio C. Biological and chemical-physical properties of root-end filling materials: a comparative study. J Conserv Dent. 2015;18:94–9.PubMedPubMedCentralCrossRefGoogle Scholar
  31. Cengiz E, Ulusoy N. Microshear bond strength of tri-calcium silicate-based cements to different restorative materials. J Adhes Dent. 2016;18:231–7.PubMedGoogle Scholar
  32. Centenaro CF, Santini MF, Da Rosa RA, et al. Effect of calcium hydroxide on the bond strength of two bioactive cements and SEM evaluation of failure patterns. Scanning. 2016;38:240–4.PubMedCrossRefGoogle Scholar
  33. Chang SW, Lee SY, Ann HJ, Kum KY, Kim EC. Effects of calcium silicate endodontic cements on biocompatibility and mineralization-inducing potentials in human dental pulp cells. J Endod. 2014;40:1194–200.PubMedCrossRefGoogle Scholar
  34. Colak H, Tokay U, Uzgur R, et al. The effect of different adhesives and setting times on bond strength between Biodentine and composite. J Appl Biomater Funct Mater. 2016;14:e217–22.PubMedGoogle Scholar
  35. Cornelio AL, Rodrigues EM, Salles LP, et al. Bioactivity of MTA Plus, Biodentine and experimental calcium silicate-based cements in human osteoblast-like cells. Int Endod J. 2015;50:39–47.CrossRefGoogle Scholar
  36. Corral Nunez CM, Bosomworth HJ, Field C, Whitworth JM, Valentine RA. Biodentine and mineral trioxide aggregate induce similar cellular responses in a fibroblast cell line. J Endod. 2014;40:406–11.PubMedCrossRefGoogle Scholar
  37. Costa F, Sousa Gomes P, Fernandes MH. Osteogenic and angiogenic response to calcium silicate-based endodontic sealers. J Endod. 2016;42:113–9.PubMedCrossRefGoogle Scholar
  38. Cuadros-Fernandez C, Lorente Rodriguez AI, Saez-Martinez S, et al. Short-term treatment outcome of pulpotomies in primary molars using mineral trioxide aggregate and Biodentine: a randomized clinical trial. Clin Oral Investig. 2015;20:1639–45.PubMedCrossRefGoogle Scholar
  39. Da Fonseca TS, Da Silva GF, Tanomaru-Filho M, et al. In vivo evaluation of the inflammatory response and IL-6 immunoexpression promoted by Biodentine and MTA Angelus. Int Endod J. 2016;49:145–53.PubMedCrossRefGoogle Scholar
  40. Daltoe MO, Paula-Silva FWG, Faccioli LH, et al. Expression of mineralization markers during pulp response to Biodentine and mineral trioxide aggregate. J Endod. 2016;42:596–603.PubMedCrossRefGoogle Scholar
  41. Darvell BW, Wu RC. “MTA”-an hydraulic silicate cement: review update and setting reaction. Dent Mater. 2011;27:407–22.PubMedCrossRefGoogle Scholar
  42. Dawood AE, Manton DJ, Parashos P, Wong RH. The effect of working time on the displacement of Biodentine beneath prefabricated stainless steel crown: a laboratory study. J Investig Clin Dent. 2015a;7:391–5.PubMedCrossRefGoogle Scholar
  43. Dawood AE, Manton DJ, Parashos P, et al. Push-out bond strength of CPP-ACP-modified calcium silicate-based cements. Dent Mater J. 2015b;34:490–4.PubMedCrossRefGoogle Scholar
  44. Dawood AE, Manton DJ, Parashos P, et al. The physical properties and ion release of CPP-ACP-modified calcium silicate-based cements. Aust Dent J. 2015c;60:434–44.CrossRefGoogle Scholar
  45. De-Deus G, Ferreira CB, Oliveira Dda S, et al. Resistance of hydraulic calcium silicate cements to dislodgment in short- and long-term assessment. J Adhes Dent. 2016;18:157–60.PubMedGoogle Scholar
  46. De Rossi A, Silva LA, Gaton-Hernandez P, et al. Comparison of pulpal responses to pulpotomy and pulp capping with Biodentine and mineral trioxide aggregate in dogs. J Endod. 2014;40:1362–9.PubMedCrossRefGoogle Scholar
  47. Deepa VL, Dhamaraju B, Bollu IP, Balaji TS. Shear bond strength evaluation of resin composite bonded to three different liners: TheraCal LC, Biodentine, and resin-modified glass ionomer cement using universal adhesive: An in vitro study. J Conserv Dent. 2016;19:166–70.PubMedPubMedCentralCrossRefGoogle Scholar
  48. Demirturk Kocasarac H, Helvacioglu Yigit D, et al. Contrast-to-noise ratio with different settings in a CBCT machine in presence of different root-end filling materials: an in vitro study. Dentomaxillofac Radiol. 2016;45:20160012.PubMedPubMedCentralCrossRefGoogle Scholar
  49. Di Fiore PM, Reyes A, Dorn SO, Cron SG, Ontiveros JC. Evaluation of a calcium silicate-based cement as a root reinforcement material for endodontically treated maxillary anterior teeth. J Prosthet Dent. 2016;115:35–41.PubMedCrossRefGoogle Scholar
  50. El-Khodary HM, Farsi DJ, Farsi NM, Zidan AZ. Sealing ability of four calcium containing cements used for repairing furcal perforations in primary molars: an in vitro study. J Contemp Dent Pract. 2015;16:733–9.PubMedCrossRefGoogle Scholar
  51. El Karim IA, Mccrudden MT, Mcgahon MK, et al. Biodentine reduces tumor necrosis factor alpha-induced TRPA1 expression in odontoblastlike cells. J Endod. 2016;42:589–95.PubMedCrossRefGoogle Scholar
  52. El Meligy OA, Allazzam S, Alamoudi NM. Comparison between Biodentine and formocresol for pulpotomy of primary teeth: a randomized clinical trial. Quintessence Int. 2016;47:571–80.PubMedGoogle Scholar
  53. Elnaghy AM. Influence of acidic environment on properties of Biodentine and white mineral trioxide aggregate: a comparative study. J Endod. 2014;40:953–7.PubMedCrossRefGoogle Scholar
  54. Elnaghy AM, Elsaka SE. Fracture resistance of simulated immature teeth filled with Biodentine and white mineral trioxide aggregate—an in vitro study. Dent Traumatol. 2016;32:116–20.PubMedCrossRefGoogle Scholar
  55. Escobar-Garcia DM, Aguirre-Lopez E, Mendez-Gonzalez V, Pozos-Guillen A. Cytotoxicity and initial biocompatibility of endodontic biomaterials (MTA and Biodentine) used as root-end filling materials. Biomed Res Int. 2016;2016:7926961.PubMedPubMedCentralCrossRefGoogle Scholar
  56. Evren OK, Altunsoy M, Tanriver M, et al. Fracture resistance of simulated immature teeth after apexification with calcium silicate-based materials. Eur J Dent. 2016;10:188–92.PubMedPubMedCentralCrossRefGoogle Scholar
  57. Gandolfi MG, Siboni F, Botero T, et al. Calcium silicate and calcium hydroxide materials for pulp capping: biointeractivity, porosity, solubility and bioactivity of current formulations. J Appl Biomater Funct Mater. 2014;13:43–60.PubMedGoogle Scholar
  58. Govindaraju L, Neelakantan P, Gutmann JL (2016) Effect of root canal irrigating solutions on the compressive strength of tricalcium silicate cements. Clin Oral Investig [Epub ahead of print].Google Scholar
  59. Gupta PK, Garg G, Kalita C, et al. Evaluation of sealing ability of Biodentine as retrograde filling material by using two different manipulation methods: an in vitro study. J Int Oral Health. 2015;7:111–4.PubMedPubMedCentralGoogle Scholar
  60. Hadrossek PH, Dammaschke T. New treatment option for an incomplete vertical root fracture—a preliminary case report. Head Face Med. 2014;10:9.PubMedPubMedCentralCrossRefGoogle Scholar
  61. Han L, Okiji T. Uptake of calcium and silicon released from calcium silicate-based endodontic materials into root canal dentine. Int Endod J. 2011;44:1081–7.PubMedCrossRefGoogle Scholar
  62. Hashem DF, Foxton R, Manoharan A, Watson TF, Banerjee A. The physical characteristics of resin composite-calcium silicate interface as part of a layered/laminate adhesive restoration. Dent Mater. 2014;30:343–9.PubMedCrossRefGoogle Scholar
  63. Hashem D, Mannocci F, Patel S, et al. Clinical and radiographic assessment of the efficacy of calcium silicate indirect pulp capping: a randomized controlled clinical trial. J Dent Res. 2015;94:562–8.PubMedPubMedCentralCrossRefGoogle Scholar
  64. Helvacioglu-Yigit D, Demirturk Kocasarac H, Bechara B, Noujeim M. Evaluation and reduction of artifacts generated by 4 different root-end filling materials by using multiple cone-beam computed tomography imaging settings. J Endod. 2016;42:307–14.PubMedCrossRefGoogle Scholar
  65. Hiremath GS, Kulkarni RD, Naik BD. Evaluation of minimal inhibitory concentration of two new materials using tube dilution method: an in vitro study. J Conserv Dent. 2015;18:159–62.PubMedPubMedCentralCrossRefGoogle Scholar
  66. Jang YE, Lee BN, Koh JT, et al. Cytotoxicity and physical properties of tricalcium silicate-based endodontic materials. Restor Dent Endod. 2014;39:89–94.PubMedPubMedCentralCrossRefGoogle Scholar
  67. Jeevani E, Jayaprakash T, Bolla N, et al. Evaluation of sealing ability of MM-MTA, endosequence, and Biodentine as furcation repair materials: UV spectrophotometric analysis. J Conserv Dent. 2014;17:340–3.PubMedPubMedCentralCrossRefGoogle Scholar
  68. Johns DA, Shivashankar VY, Shobha K, Johns M. An innovative approach in the management of palatogingival groove using Biodentine and platelet-rich fibrin membrane. J Conserv Dent. 2014;17:75–9.PubMedPubMedCentralCrossRefGoogle Scholar
  69. Jung JY, Woo SM, Lee BN, et al. Effect of Biodentine and Bioaggregate on odontoblastic differentiation via mitogen-activated protein kinase pathway in human dental pulp cells. Int Endod J. 2015;48:177–84.PubMedCrossRefGoogle Scholar
  70. Jung S, Mielert J, Kleinheinz J, Dammaschke T. Human oral cells’ response to different endodontic restorative materials: an in vitro study. Head Face Med. 2014;10:55.PubMedPubMedCentralCrossRefGoogle Scholar
  71. Karypidou A, Chatzinikolaou ID, Kouros P, Koulaouzidou E, Economides N. Management of bilateral invasive cervical resorption lesions in maxillary incisors using a novel calcium silicate-based cement: a case report. Quintessence Int. 2016;47:637–42.PubMedGoogle Scholar
  72. Kaup M, Dammann CH, Schafer E, Dammaschke T. Shear bond strength of Biodentine, ProRoot MTA, glass ionomer cement and composite resin on human dentine ex vivo. Head Face Med. 2015a;11:14.PubMedPubMedCentralCrossRefGoogle Scholar
  73. Kaup M, Schafer E, Dammaschke T. An in vitro study of different material properties of Biodentine compared to ProRoot MTA. Head Face Med. 2015b;11:16.PubMedPubMedCentralCrossRefGoogle Scholar
  74. Kayahan MB, Nekoofar MH, Mccann A, et al. Effect of acid etching procedures on the compressive strength of 4 calcium silicate-based endodontic cements. J Endod. 2013;39:1646–8.PubMedCrossRefGoogle Scholar
  75. Kenchappa M, Gupta S, Gupta P, Sharma P. Dentine in a capsule: clinical case reports. J Indian Soc Pedod Prev Dent. 2015;33:250–4.PubMedCrossRefGoogle Scholar
  76. Keskin C, Demiryurek EO, Ozyurek T. Color stabilities of calcium silicate-based materials in contact with different irrigation solutions. J Endod. 2015;41:409–11.PubMedCrossRefGoogle Scholar
  77. Khetarpal A, Chaudhary S, Talwar S, Verma M. Endodontic management of open apex using Biodentine as a novel apical matrix. Indian J Dent Res. 2014;25:513–6.PubMedCrossRefGoogle Scholar
  78. Kim JR, Nosrat A, Fouad AF. Interfacial characteristics of Biodentine and MTA with dentine in simulated body fluid. J Dent. 2015;43:241–7.PubMedCrossRefGoogle Scholar
  79. Kim J, Song YS, Min KS, et al. Evaluation of reparative dentin formation of ProRoot MTA, Biodentine and BioAggregate using micro-CT and immunohistochemistry. Restor Dent Endod. 2016;41:29–36.PubMedPubMedCentralCrossRefGoogle Scholar
  80. Kohli MR, Yamaguchi M, Setzer FC, Karabucak B. Spectrophotometric analysis of coronal tooth discoloration induced by various bioceramic cements and other endodontic materials. J Endod. 2015;41:1862–6.PubMedCrossRefGoogle Scholar
  81. Koruyucu M, Topcuoglu N, Tuna EB, et al. An assessment of antibacterial activity of three pulp capping materials on Enterococcus faecalis by a direct contact test: an in vitro study. Eur J Dent. 2015;9:240–5.PubMedPubMedCentralCrossRefGoogle Scholar
  82. Kucukkaya S, Gorduysus MO, Zeybek ND, Muftuoglu SF. In vitro cytotoxicity of calcium silicate-based endodontic cement as root-end filling materials. Scientifica. 2016;2016(2016):5.Google Scholar
  83. Kucukkaya Eren S, Aksel H, Serper A. Effect of placement technique on the push-out bond strength of calcium-silicate based cements. Dent Mater J. 2016;35:742–7.PubMedCrossRefGoogle Scholar
  84. Kum KY, Kim EC, Yoo YJ, et al. Trace metal contents of three tricalcium silicate materials: MTA Angelus, Micro Mega MTA and Bioaggregate. Int Endod J. 2014;47:704–10.PubMedCrossRefGoogle Scholar
  85. Kusum B, Rakesh K, Richa K. Clinical and radiographical evaluation of mineral trioxide aggregate, Biodentine and propolis as pulpotomy medicaments in primary teeth. Restor Dent Endod. 2015;40:276–85.PubMedPubMedCentralCrossRefGoogle Scholar
  86. Lee BN, Lee KN, Koh JT, et al. Effects of 3 endodontic bioactive cements on osteogenic differentiation in mesenchymal stem cells. J Endod. 2014;40:1217–22.PubMedCrossRefGoogle Scholar
  87. Lenherr P, Allgayer N, Weiger R, et al. Tooth discoloration induced by endodontic materials: a laboratory study. Int Endod J. 2012;45:942–9.PubMedCrossRefGoogle Scholar
  88. Li X, Pongprueksa P, Van Landuyt K, et al. Correlative micro-Raman/EPMA analysis of the hydraulic calcium silicate cement interface with dentin. Clin Oral Investig. 2015;20:1663–73.PubMedCrossRefGoogle Scholar
  89. Li X, Yoshihara K, De Munck J, et al. (2016) Modified tricalcium silicate cement formulations with added zirconium oxide. Clin Oral Investig [Epub ahead of print].Google Scholar
  90. Luo Z, Li D, Kohli MR, et al. Effect of Biodentine (TM) on the proliferation, migration and adhesion of human dental pulp stem cells. J Dent. 2014a;42:490–7.PubMedCrossRefGoogle Scholar
  91. Luo ZR, Kohli MR, Yu Q, et al. Biodentine induces human dental pulp stem cell differentiation through mitogen-activated protein kinase and calcium-/calmodulin-dependent protein kinase II pathways. J Endod. 2014b;40:937–42.PubMedCrossRefGoogle Scholar
  92. Malkondu O, Karapinar Kazandag M, Kazazoglu E. A review on Biodentine, a contemporary dentine replacement and repair material. Biomed Res Int. 2014;2014:160951.PubMedPubMedCentralCrossRefGoogle Scholar
  93. Mandava P, Bolla N, Thumu J, Vemuri S, Chukka S. Microleakage evaluation around retrograde filling materials prepared using conventional and ultrasonic techniques. J Clin Diagn Res. 2015;9:43–6.Google Scholar
  94. Marconyak LJ Jr, Kirkpatrick TC, Roberts HW, et al. A comparison of coronal tooth discoloration elicited by various endodontic reparative materials. J Endod. 2016;42:470–3.PubMedCrossRefGoogle Scholar
  95. Martens L, Rajasekharan S, Cauwels R. Pulp management after traumatic injuries with a tricalcium silicate-based cement (Biodentine): a report of two cases, up to 48 months follow-up. Eur Arch Paediatr Dent. 2015;16:491–6.PubMedCrossRefGoogle Scholar
  96. Martens L, Rajasekharan S, Cauwels R. Endodontic treatment of trauma-induced necrotic immature teeth using a tricalcium silicate-based bioactive cement. A report of 3 cases with 24-month follow-up. Eur J Paediatr Dent. 2016;17:24–8.PubMedGoogle Scholar
  97. Mori GG, Teixeira LM, De Oliveira DL, Jacomini LM, Da Silva SR. Biocompatibility evaluation of Biodentine in subcutaneous tissue of rats. J Endod. 2014;40:1485–8.PubMedCrossRefGoogle Scholar
  98. Nadig PP, Agrawal IS, Agrawal VS, Srinivasan SC. Palato-radicular groove: a rare entity in maxillary central incisor leading to endo-perio lesion. J Clin Diagn Res. 2016;10:14–5.Google Scholar
  99. Nagas E, Cehreli ZC, Uyanik MO, Vallittu PK, Lassila LV. Effect of several intracanal medicaments on the push-out bond strength of ProRoot MTA and Biodentine. Int Endod J. 2016a;49:184–8.PubMedCrossRefGoogle Scholar
  100. Nagas E, Cehreli ZC, Uyanik O, Vallittu PK, Lassila LV. Reinforcing effect of glass fiber-incorporated ProRoot MTA and Biodentine as intraorifice barriers. J Endod. 2016b;42:1673–6.PubMedCrossRefGoogle Scholar
  101. Naik M, De Ataide Ide N, Fernandes M, Lambor R. Treatment of combined endodontic: periodontic lesion by sealing of palato-radicular groove using Biodentine. J Conserv Dent. 2014;17:594–7.PubMedPubMedCentralCrossRefGoogle Scholar
  102. Naik MM, De Ataide Ide N, Fernandes M, Lambor R. Assessment of apical seal obtained after irrigation of root end cavity with MTAD followed by subsequent retrofilling with MTA and Biodentine: an in vitro study. J Conserv Dent. 2015;18:132–5.PubMedPubMedCentralCrossRefGoogle Scholar
  103. Nanjappa AS, Ponnappa KC, Nanjamma KK, et al. Sealing ability of three root-end filling materials prepared using an erbium: yttrium aluminium garnet laser and endosonic tip evaluated by confocal laser scanning microscopy. J Conserv Dent. 2015;18:327–30.PubMedPubMedCentralCrossRefGoogle Scholar
  104. Natale LC, Rodrigues MC, Xavier TA, et al. Ion release and mechanical properties of calcium silicate and calcium hydroxide materials used for pulp capping. Int Endod J. 2015;48:89–94.PubMedCrossRefGoogle Scholar
  105. Nayak G, Hasan MF. Biodentine-a novel dentinal substitute for single visit apexification. Restor Dent Endod. 2014;39:120–5.PubMedPubMedCentralCrossRefGoogle Scholar
  106. Niranjan B, Shashikiran ND, Dubey A, Singla S, Gupta N. Biodentine-A new novel bio-inductive material for treatment of traumatically injured tooth (single visit apexification). J Clin Diagn Res. 2016;10:3–4.CrossRefGoogle Scholar
  107. Niranjani K, Prasad MG, Vasa AA, et al. Clinical evaluation of success of primary teeth pulpotomy using mineral trioxide aggregate((R)), laser and Biodentine(TM)—an in vivo study. J Clin Diagn Res. 2015;9:35–7.Google Scholar
  108. Nowicka A, Wilk G, Lipski M, Kolecki J, Buczkowska-Radlinska J. Tomographic evaluation of reparative dentin formation after direct pulp capping with Ca(OH)2, MTA, Biodentine, and dentin bonding system in human teeth. J Endod. 2015;41:1234–40.PubMedCrossRefGoogle Scholar
  109. Odabas ME, Bani M, Tirali RE. Shear bond strengths of different adhesive systems to Biodentine. Sci World J. 2013;2013:626103.CrossRefGoogle Scholar
  110. Pawar AM, Kokate SR, Shah RA. Management of a large periapical lesion using Biodentine() as retrograde restoration with eighteen months evident follow up. J Conserv Dent. 2013;16:573–5.PubMedPubMedCentralCrossRefGoogle Scholar
  111. Peters OA, Galicia J, Arias A, et al. Effects of two calcium silicate cements on cell viability, angiogenic growth factor release, and related gene expression in stem cells from the apical papilla. Int Endod J. 2015;49:1132–40.PubMedCrossRefGoogle Scholar
  112. Poggio C, Arciola CR, Beltrami R, et al. Cytocompatibility and antibacterial properties of capping materials. Sci World J. 2014a;2014:181945.CrossRefGoogle Scholar
  113. Poggio C, Ceci M, Beltrami R, et al. Biocompatibility of a new pulp capping cement. Ann Stomatol (Roma). 2014b;5:69–76.Google Scholar
  114. Poggio C, Beltrami R, Colombo M, et al. In vitro antibacterial activity of different pulp capping materials. J Clin Exp Dent. 2015a;7:e584–8.PubMedPubMedCentralGoogle Scholar
  115. Poggio C, Ceci M, Dagna A, et al. In vitro cytotoxicity evaluation of different pulp capping materials: a comparative study. Arh Hig Rada Toksikol. 2015b;66:181–8.PubMedCrossRefGoogle Scholar
  116. Pruthi PJ, Dharmani U, Roongta R, Talwar S. Management of external perforating root resorption by intentional replantation followed by Biodentine restoration. Dent Res J (Isfahan). 2015;12:488–93.CrossRefGoogle Scholar
  117. Rajasekharan S, Martens LC, Cauwels RG, Verbeeck RM. Biodentine material characteristics and clinical applications: a review of the literature. Eur Arch Paediatr Dent. 2014;15:147–58.PubMedCrossRefGoogle Scholar
  118. Rajasekharan S, Martens L, Vandenbulcke J, et al. Efficacy of three different pulpotomy agents in primary molars—a randomised control trial. Int Endod J. 2016;50:215–28.PubMedCrossRefGoogle Scholar
  119. Raju VG, Venumbaka NR, Mungara J, et al. Comparative evaluation of shear bond strength and microleakage of tricalcium silicate-based restorative material and radioopaque posterior glass ionomer restorative cement in primary and permanent teeth: an in vitro study. J Indian Soc Pedod Prev Dent. 2014;32:304–10.PubMedCrossRefGoogle Scholar
  120. Ramos JC, Palma PJ, Nascimento R, et al. 1-year in vitro evaluation of tooth discoloration induced by 2 calcium silicate-based cements. J Endod. 2016;42:1403–7.PubMedCrossRefGoogle Scholar
  121. Saberi EA, Karkehabadi H, Mollashahi NF. Cytotoxicity of various endodontic materials on stem cells of human apical papilla. Iran Endod J. 2016;11:17–22.PubMedGoogle Scholar
  122. Salzano S, Tirone F. Conservative nonsurgical treatment of class 4 invasive cervical resorption: a case series. J Endod. 2015;41:1907–12.PubMedCrossRefGoogle Scholar
  123. Samyuktha V, Ravikumar P, Nagesh B, et al. Cytotoxicity evaluation of root repair materials in human-cultured periodontal ligament fibroblasts. J Conserv Dent. 2014;17:467–70.PubMedPubMedCentralCrossRefGoogle Scholar
  124. Setbon HM, Devaux J, Iserentant A, Leloup G, Leprince JG. Influence of composition on setting kinetics of new injectable and/or fast setting tricalcium silicate cements. Dent Mater. 2014;30:1291–303.PubMedCrossRefGoogle Scholar
  125. Sharma S, Deepak P, Vivek S, Ranjan Dutta S. Palatogingival groove: recognizing and managing the hidden tract in a maxillary incisor: a case report. J Int Oral Health. 2015;7:110–4.PubMedPubMedCentralGoogle Scholar
  126. Shokouhinejad N, Nekoofar MH, Pirmoazen S, Shamshiri AR, Dummer PM. Evaluation and comparison of occurrence of tooth discoloration after the application of various calcium silicate-based cements: an ex vivo study. J Endod. 2016;42:140–4.PubMedCrossRefGoogle Scholar
  127. Silva EJ, Carvalho NK, Zanon M, et al. Push-out bond strength of MTA HP, a new high-plasticity calcium silicate-based cement. Braz Oral Res. 2016a;30(1).  https://doi.org/10.1590/1807-3107BOR-2016.vol30.0084
  128. Silva EJ, Senna PM, De-Deus G, Zaia AA. Cytocompatibility of Biodentine using a three-dimensional cell culture model. Int Endod J. 2016b;49:574–80.PubMedCrossRefGoogle Scholar
  129. Simsek N, Alan H, Ahmetoglu F, et al. Assessment of the biocompatibility of mineral trioxide aggregate, bioaggregate, and Biodentine in the subcutaneous tissue of rats. Niger J Clin Pract. 2015;18:739–43.PubMedCrossRefGoogle Scholar
  130. Simsek N, Bulut ET, Ahmetoglu F, Alan H. Determination of trace elements in rat organs implanted with endodontic repair materials by ICP-MS. J Mater Sci Mater Med. 2016;27:46.PubMedCrossRefGoogle Scholar
  131. Singh S, Podar R, Dadu S, Kulkarni G, Purba R. Solubility of a new calcium silicate-based root-end filling material. J Conserv Dent. 2015;18:149–53.PubMedPubMedCentralCrossRefGoogle Scholar
  132. Sinha N, Singh B, Patil S. Cone beam-computed topographic evaluation of a central incisor with an open apex and a failed root canal treatment using one-step apexification with Biodentine: a case report. J Conserv Dent. 2014;17:285–9.PubMedPubMedCentralCrossRefGoogle Scholar
  133. Soni HK. Biodentine pulpotomy in mature permanent molar: a case report. J Clin Diagn Res. 2016;10:9–11.Google Scholar
  134. Soundappan S, Sundaramurthy JL, Raghu S, Natanasabapathy V. Biodentine versus mineral trioxide aggregate versus intermediate restorative material for retrograde root end filling: an invitro study. J Dent (Tehran). 2014;11:143–9.Google Scholar
  135. Tanalp J, Karapinar-Kazandag M, Dolekoglu S, Kayahan MB. Comparison of the radiopacities of different root-end filling and repair materials. Sci World J. 2013;2013:594950.CrossRefGoogle Scholar
  136. Togaru H, Muppa R, Srinivas N, et al. Clinical and radiographic evaluation of success of two commercially available pulpotomy agents in primary teeth: an in vivo study. J Contemp Dent Pract. 2016;17:557–63.PubMedGoogle Scholar
  137. Topcuoglu G, Topcuoglu HS. Regenerative endodontic therapy in a single visit using platelet-rich plasma and Biodentine in necrotic and asymptomatic immature molar teeth: a report of 3 cases. J Endod. 2016;42:1344–6.PubMedCrossRefGoogle Scholar
  138. Tziafa C, Koliniotou-Koumpia E, Papadimitriou S, Tziafas D. Dentinogenic responses after direct pulp capping of miniature swine teeth with Biodentine. J Endod. 2014;40:1967–71.PubMedCrossRefGoogle Scholar
  139. Ulusoy OI, Paltun YN, Guven N, Celik B. Dislodgement resistance of calcium silicate-based materials from root canals with varying thickness of dentine. Int Endod J. 2015;49:1188–93.PubMedCrossRefGoogle Scholar
  140. Umashetty G, Hoshing U, Patil S, Ajgaonkar N. Management of inflammatory internal root resorption with Biodentine and thermoplasticised gutta-percha. Case Rep Dent. 2015;2015:452609.PubMedPubMedCentralGoogle Scholar
  141. Ustun Y, Topcuoglu HS, Akpek F, Aslan T. The effect of blood contamination on dislocation resistance of different endodontic reparative materials. J Oral Sci. 2015;57:185–90.PubMedCrossRefGoogle Scholar
  142. Valles M, Roig M, Duran-Sindreu F, Martinez S, Mercade M. Color stability of teeth restored with Biodentine: a 6-month in vitro study. J Endod. 2015;41:1157–60.PubMedCrossRefGoogle Scholar
  143. Vemisetty H, Reddy SJ, et al. Comparative evaluation of marginal adaptation of Biodentine(TM) and other commonly used root end filling materials-an in vitro study. J Clin Diagn Res. 2014;8:243–5.PubMedPubMedCentralGoogle Scholar
  144. Vidal K, Martin G, Lozano O, et al. Apical closure in apexification: a review and case report of apexification treatment of an immature permanent tooth with Biodentine. J Endod. 2016;42:730–4.PubMedCrossRefGoogle Scholar
  145. Villat C, Grosgogeat B, Seux D, Farge P. Conservative approach of a symptomatic carious immature permanent tooth using a tricalcium silicate cement (Biodentine): a case report. Restor Dent Endod. 2013;38:258–62.PubMedPubMedCentralCrossRefGoogle Scholar
  146. Widbiller M, Lindner SR, Buchalla W, et al. Three-dimensional culture of dental pulp stem cells in direct contact to tricalcium silicate cements. Clin Oral Investig. 2016;20:237–46.PubMedCrossRefGoogle Scholar
  147. Yoldas SE, Bani M, Atabek D, Bodur H. Comparison of the potential discoloration effect of bioaggregate, Biodentine, and white mineral trioxide aggregate on bovine teeth In vitro research. J Endod. 2016;42:1815–8.PubMedCrossRefGoogle Scholar
  148. Zhabuawala MS, Nadig RR, Pai VS, Gowda Y. Comparison of fracture resistance of simulated immature teeth with an open apex using Biodentine and composite resin: an in vitro study. J Indian Soc Pedod Prev Dent. 2016;34:377–82.PubMedCrossRefGoogle Scholar

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© European Academy of Paediatric Dentistry 2018

Authors and Affiliations

  1. 1.Department of Paediatric Dentistry and special care, PAECOMEDIS Research ClusterGhent UniversityGhentBelgium
  2. 2.Paediatric Oral Health Research Group, School of DentistryThe University of Western AustraliaPerthAustralia

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