European Archives of Paediatric Dentistry

, Volume 19, Issue 1, pp 47–55 | Cite as

Efficacy of a single session protocol for endodontic treatment in primary teeth: in vivo study

  • T. C. TrichesEmail author
  • G. S. Zimmermann
  • S. F. T. de Freitas
  • M. Ximenes
  • R. S. Vieira
  • M. M. R. Cordeiro
Original Scientific Article



To determine the efficacy of a single session protocol (SSP) in the reduction of septic content of primary teeth root canals and identify the persistence of bacterial species associated with unsuccessful treatment.


Primary teeth root canals (16) with pulp necrosis and peri-radicular lesions were treated. Samples were collected at baseline (T1), and after chemo-mechanical preparation, before filling (T2). Identification of the microorganisms was determined using checkerboard DNA–DNA hybridisation. Statistical analysis: Wilcoxon test was applied for comparison of mean number of species, proportion and mean count of each species between the evaluation times.


Significant reductions were found in the mean number of bacteria species between T1 and T2 (p < 0.05), but not for the reduction in proportion (p > 0.05). There was a reduction (6.0–4.6) of the mean number of species associated with failure, without statistical significance.


The SSP was capable of significantly reducing the septic content, even though, many of the bacteria associated with failure persisted at the time of root canal filling.


Dental pulp necrosis DNA probes Failure Primary teeth Root canal therapy 



The authors are grateful to the National Council for the Improvement of Higher Education (CAPES/REUNI) for funding granted to the present study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© European Academy of Paediatric Dentistry 2018

Authors and Affiliations

  • T. C. Triches
    • 1
    • 2
    Email author
  • G. S. Zimmermann
    • 1
  • S. F. T. de Freitas
    • 3
  • M. Ximenes
    • 1
  • R. S. Vieira
    • 1
  • M. M. R. Cordeiro
    • 1
  1. 1.Department of DentistryFederal University of Santa Catarina (UFSC)FlorianópolisBrazil
  2. 2.Terra RoxaBrazil
  3. 3.Department of Public HealthUFSCFlorianópolisBrazil

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