To compare the effectiveness of different decontamination methods on tried-in preformed metal crowns (PMCs).
Sixty unused PMCs and 90 tried-in PMCs from patients were assessed for contamination after culturing for 24 h on liquid media, solid media and differential media for identification of Streptococcus mutans, Staphylococcus aureus, and Escherichia coli. Subsequently, these PMCs were divided equally into the following six groups: autoclave (121 °C, 15 psi for 15 min), 5% sodium hypochlorite (5 min), 5% glutaraldehyde (5 min), 70% isopropyl alcohol (1 min) and normal saline (5 min). The contamination was reassessed, and the Log10 counts were compared to the pre-decontamination levels using one way ANOVA and paired t-test at a significance level of p < 0.05.
The mean percentage reduction in colony counts was significantly more in the autoclave group compared to glutaraldehyde or sodium hypochlorite groups, glass bead, isopropyl alcohol, and normal saline in this decreasing order.
PMCs supplied by the manufacturer were found to be sterile and can be used directly on patients. The autoclave was the best method of sterilisation, although it did not eliminate the microbes 100%; followed by 5% glutaraldehyde and 5% sodium hypochlorite.
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The study was approved by Institution Review board of Sudha Rustagi College of Dental Sciences and Research, Faridabad.
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The authors deny any conflicts of interest.
Research involving human participants and/or animals
All procedures performed in this study were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed consent was taken from all the participants of the study.
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Darshan, V., Indushekar, K.R., Saraf, B.G. et al. A comparison of decontamination methods of tried-in preformed metal crowns: an in-vivo study. Eur Arch Paediatr Dent 20, 537–544 (2019). https://doi.org/10.1007/s40368-018-00414-4
- Preformed Metal
- Sodium hypochlorite