Evaluation of remineralisation potential of experimental nano hydroxyapatite pastes using scanning electron microscope with energy dispersive X-ray analysis: an in-vitro trial
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To analyse the remineralisation potential of experimental nano hydroxyapatite (nHAP) paste on artificial caries lesions using scanning electron microscope (SEM) with energy dispersive X-ray analysis (EDX).
Sixty-five enamel specimens were randomly divided into 4 test groups (n = 15): 1% commercially available nHAP paste (CnHAP) group, 1% experimental nHAP paste (EnHAP) group, 10% EnHAP group, casein phosphopeptide–amorphous calcium phosphate (CPP–ACP) group and control group (n = 5). Artificial carious lesions were induced in all the specimens and treated with respective paste twice daily for 14 days. The specimens were subjected to EDX analysis at baseline, after demineralisation and after remineralisation. The surface characteristics of the remineralised enamel specimens were evaluated by SEM.
All the test groups showed significant change in calcium and phosphorus weight percentage (wt%) after remineralisation. The 10 % EnHAP showed higher mean value of calcium and phosphorus wt% followed by 1% CnHAP, 1% EnHAP and CPP–ACP. After remineralisation, both 10% EnHAP and CPP–ACP showed favourable enamel surface changes in SEM analysis.
Commercially available and experimental nHAP have the potential to remineralise artificially induced carious lesions.
KeywordsTooth remineralisation Nano hydroxyapatite paste Energy dispersive X-ray Scanning electron microscope
Compliance with ethical standards
Conflict of interest
All three authors declare no conflict of interest.
- de Carvalho FG, Vieira BR, Santos RL, et al. In vitro effects of nano-hydroxyapatite paste on initial enamel carious lesions. Pediatr Dent. 2014;36(3):85–9.Google Scholar
- Fejerskov O, Kidd AME. Defining the disease: an introduction. In: Fejerskov O, Kidd EA, Nyvad B, Baelum V, editors. Dental caries: the disease and its clinical management. 2nd ed. Copenhagen: Blackwell Munksgaard publication; 2008.Google Scholar
- Haghgoo R, Rezvani MB, SalehiZeinabadi M. Comparison of nano-hydroxyapatite and sodium fluoride mouthrinse for remineralization of incipient carious lesions. J Dent. 2014;11(4):406–10.Google Scholar
- Hedge S, Roma M, Shetty D. Non fluoridated remineralizing agents in dentistry. J Pharm Sci Res. 2016;8(8):884–7.Google Scholar
- Kidd AME. Introduction: what is caries. In: Kidd EA, editors. Essentials of dental caries: the disease and its management. 3rd ed. Oxford: Oxford medical Publication; 2005.Google Scholar
- Kim MY, Kwon HK, Choi CH, Kim BI. Combined effects of nano-hydroxyapatite and NaF on remineralization of early caries lesion. Key Eng Mater. 2007;1347:330–2.Google Scholar
- Najibfard K, Ramalingam K, Chedjieu I, Amaechi BT. Remineralization of early caries by a nano-hydroxyapatite dentifrice. J Clin Dent. 2011;22(5):139–43.Google Scholar
- Naveena P, Nagarathana C, Sakunthala BK. Remineralizing agent—then and now—an update. Dentistry. 2014;4(9):1–5.Google Scholar
- Shetty PA, Shetty D, Shetty S. A comparison of clinical efficacy of dentifrices containing calcium sodium phosphosilicate, nanoparticle hydroxyapatite and a dentifrice containing casein phosphopeptide amorphous calcium phosphate on dentinal hypersensitivity—a comparative triple blind randomized study. Adv Hum Biol. 2014;4:57–64.Google Scholar
- Verma P, Gupta U, Dodwad V, et al. Evaluation of the clinical efficacy of a new desensitizing toothpaste containing nano-crystalline hydroxyapatite in dentine hypersensitivity patients: a double blind randomized controlled clinical trial. J Dent Spec. 2013;1(2):47–54.Google Scholar