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European Archives of Paediatric Dentistry

, Volume 18, Issue 4, pp 251–261 | Cite as

Quantitative analysis of S. mutans, Lactobacillus and Bifidobacterium found in initial and mature plaques in Thai children with early childhood caries

  • K. MitrakulEmail author
  • S. Chanvitan
  • A. Jeamset
  • K. Vongsawan
Original Scientific Article

Abstract

Aims

To quantify Streptococcus mutans, lactobacillus and bifidobacterium in initial and mature plaque collected from children with severe early childhood caries (S-ECC) and caries-free (CF) groups and to analyse the association between these bacteria and caries-related factors in each group.

Study design

A collection of 120 initial and overnight supra-gingival plaques were collected from Thai children aged 2–5 years-old (S-ECC = 60, CF = 60). Plaque, gingival indices and decayed, missing, filled tooth (dmft) scores were recorded. A questionnaire was used to assess the parents’ attitudes and behaviour regarding the child’s oral hygiene care and diet.

Methods

After DNA extraction, quantitative real-time polymerase chain reaction (PCR) using fluorescent dye (SYBR green) was performed.

Results

Levels of Streptococcus mutans, lactobacillus and bifidobacterium in both initial and mature plaques of S-ECC were significantly higher than those from the caries-free group (p < 0.05). The ratio of S. mutans, lactobacillus, and bifidobacterium to the total bacteria in S-ECC was significantly higher than in the caries-free group (p < 0.05). Levels of lactobacillus and bifidobacterium in both plaques significantly correlated with dmft scores and the plaque index, while S. mutans levels only correlated with dmft scores (p < 0.05). Factors that were significantly associated with caries were parents’s education, duration of bottle feeding, especially during sleeping and the frequency of consuming cariogenic food between meals (p < 0.05).

Conclusion

Levels of S. mutans, lactobacillus, bifidobacterium and the ratio of these bacteria to total bacteria in both initial and mature plaques were significantly higher in children with S-ECC and related to dmft scores, oral hygiene and dietary habits.

Keywords

Early childhood caries S. mutans Lactobacillus Bifidobacterium Dental plaque Polymerase chain reaction 

Notes

Compliance with ethical standards

Conflict of interest

All authors declare that he/she have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© European Academy of Paediatric Dentistry 2017

Authors and Affiliations

  • K. Mitrakul
    • 1
    Email author
  • S. Chanvitan
    • 1
  • A. Jeamset
    • 1
  • K. Vongsawan
    • 1
  1. 1.Department of Paediatric Dentistry, Faculty of DentistryMahidol UniversityBangkokThailand

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