European Archives of Paediatric Dentistry

, Volume 17, Issue 4, pp 231–237 | Cite as

Remineralizing potential of CPP-ACP applied for 1 min in vitro

  • V. Gopalakrishnan
  • R. P. AnthonappaEmail author
  • N. M. King
  • A. Itthagarun
Original Scientific Article



To evaluate (1) the remineralizing potential of Tooth Mousse® (TM) on artificial carious lesions, when applied for 1 min, and (2) the benefit of a fluoridated dentifrice prior to TM application.


Demineralisation caries-like lesions 120–200 μm deep were produced by placing molars in demineralizing solution for 96 h, sections 100–150 μm thick were then randomly assigned to four groups. Specimens were treated thrice daily with a non-fluoridated (Group A), or fluoridated dentifrice (1000 ppm) (Group B), or TM (Group C), or a fluoridated dentifrice followed by TM (Group D) then subjected to a 10-day pH cycling model. Lesion evaluation involved polarizing light microscopy and microradiography.


Application of TM in isolation (Group C) for 1 min resulted in significant increase in lesion depth (LD), however, an increase in mineral content deposition (V max) was not significant. Post treatment LD increases were greatest in Group A, then Group C and Group B respectively. A decrease in LD (by 19.9 %) was noted only after fluoridated dentifrice and TM (Group D) were applied consecutively for 1 min each (p < 0.05). Mineral content deposition was also greatest in this group (Group D) and both V max and LD changes were statistically significant when compared to other groups.


TM alone failed to remineralize carious lesions when applied for 1 min. However, remineralization occurred after a prior application of a fluoridated dentifrice.


Remineralization Demineralization Dental caries Fluoride Dentifrice CPP-ACP 



None to declare.

Compliance with ethical standards

Conflict of interest

Author Gopalkrishnan declares that she has no conflict of interest. Author Anthonappa declares that he has no conflict of interest. Author King declares that he has no conflict of interest. Author Itthagarun declares that she has no conflict of interest.


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Copyright information

© European Academy of Paediatric Dentistry 2016

Authors and Affiliations

  • V. Gopalakrishnan
    • 1
  • R. P. Anthonappa
    • 1
    Email author
  • N. M. King
    • 1
  • A. Itthagarun
    • 2
  1. 1.Paediatric DentistrySchool of Dentistry, University of Western AustraliaNedlandsAustralia
  2. 2.Paediatric DentistrySchool of Dentistry and Oral Health, Griffith UniversityGold CoastAustralia

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