European Archives of Paediatric Dentistry

, Volume 17, Issue 1, pp 45–51 | Cite as

Fluoride release and recharging ability of new dental sealants

  • D. DionysopoulosEmail author
  • T. Sfeikos
  • K. Tolidis
Original Scientific Article



This in vitro investigation was to evaluate the fluoride release and recharge abilities of three recently introduced dental fissure sealants (FS).


Three dental FS were assessed: Teethmate F-1 (Kuraray), Fissurit F (Voco), BeautiSealant (Shofu), and a conventional glass ionomer cement FX-II (Shofu) as a control. Eight cylindrical specimens (7 × 2 mm) of each material were made and immersed individually in 5 ml de-ionized water in plastic containers. Fluoride release of the tested materials was evaluated during the experimental period (28 days) utilising a fluoride ion-selective electrode. At the end of the test period, the specimens were soaked for 5 min in a fluoride solution (0.05 % NaF) and fluoride release was evaluated for the next 5 days. The data were statistically analysed using one-way ANOVA and Bonferroni post hoc test and the level of significance was preset at α = 0.05.


The total fluoride release over the 28-day period for each material was: FX-II (408.6 ± 45.66 μg/cm2) > Teethmate F-1 (89.45 ± 12.32 μg/cm2) > Fissurit F (68.62 ± 8.72 μg/cm2) > BeautiSealant (33.32 ± 4.91 μg/cm2), (p < 0.05). After the re-fluoridation of the specimens with 0.05 % NaF solution the cumulative fluoride re-release during the 5-day period for each material was: FX-II (99.53 ± 13.21 μg/cm2) > Teethmate F-1 (9.76 ± 1.62 μg/cm2) > BeautiSealant (5.69 ± 0.89 μg/cm2) > Fissurit F (4.76 ± 0.72 μg/cm2), (p < 0.05).


The three tested sealants exhibited different capabilities to release and uptake fluoride ions after re-fluoridation but significantly lower than the glass ionomer tested.


Dental sealants Fluoride recharge Fluoride release Giomer Glass ionomer Resin-based materials 


  1. Azarpazhooh A, Main PA. Pit and fissure sealants in the prevention of dental caries in children and adolescents: a systematic review. J Can Dent Assoc. 2008;74:171–7.PubMedGoogle Scholar
  2. Beauchamp J, Caufield PW, Crall JJ, et al. Evidence-based clinical recommendations for the use of pit-and-fissure sealants: a report of the American Dental Association Council on Scientific Affairs. J Am Dent Assoc. 2008;139:257–68.CrossRefPubMedGoogle Scholar
  3. Bell A, Creanor SL, Foye RH, Saunders WP. The effect of saliva on fluoride release by a glass-ionomer filling material. J Oral Rehabil. 1999;26:407–12.CrossRefPubMedGoogle Scholar
  4. Bertacchini SM, Abate PF, Blank A, Baglieto MF, Macchi RL. Solubility and fluoride release in ionomers and compomers. Quintessence Int. 1999;30:193–7.PubMedGoogle Scholar
  5. Choudhary P, Tandon S, Ganesh M, Mehra A. Evaluation of the remineralization potential of amorphous calcium phosphate and fluoride containing pit and fissure sealants using scanning electron microscopy. Indian J Dent Res. 2012;23:157–63.CrossRefPubMedGoogle Scholar
  6. Czarnecka B, Limanowska-Shaw H, Nicholson JW. Buffering and ion-release by a glass-ionomer cement under near-neutral and acidic conditions. Biomaterials. 2002;23:2783–8.CrossRefPubMedGoogle Scholar
  7. Dionysopoulos D. The effect of fluoride-releasing restorative materials on inhibition of secondary caries formation. Fluoride. 2014;47:258–65.Google Scholar
  8. Dionysopoulos P, Kotsanos N, Pataridou A. Fluoride release and uptake by four new fluoride releasing restorative materials. J Oral Rehabil. 2003;30:866–72.CrossRefPubMedGoogle Scholar
  9. Dionysopoulos D, Koliniotou-Koumpia E, Helvatzoglou-Antoniades M, Kotsanos N. Fluoride release and recharge ability of contemporary fluoride-containing restorative materials and dental adhesives. Dent Mater J. 2013;32:296–304.CrossRefPubMedGoogle Scholar
  10. Fan Y, Townsend J, Wang Y, et al. Formulation and characterization of antibacterial fluoride-releasing sealants. Pediatr Dent. 2013;35:13–8.PubMedCentralGoogle Scholar
  11. FDI Commission. Mouthrinses and dental caries. Int Dent J. 2002;52:337–45.CrossRefGoogle Scholar
  12. Garcia-Godoy F, Abarzua I, De Goes MF, Chan DC. Fluoride release from fissure sealants. J Clin Pediatr Dent. 1997;22:45–9.PubMedGoogle Scholar
  13. Han L, Cv E, Li M, et al. Effect of fluoride mouth rinse on fluoride releasing and recharging from aesthetic dental materials. Dent Mater J. 2002;21:285–95.CrossRefPubMedGoogle Scholar
  14. Hatibovic-Kofman S, Koch G, Ekstrand J. Glass ionomer materials as a rechargeable fluoride-release system. Int J Paediatr Dent. 1997;7:65–73.CrossRefPubMedGoogle Scholar
  15. Hayacibara MF, Ambrozano GM, Cury JA. Simultaneous release of fluoride and aluminum from dental materials in various immersion media. Oper Dent. 2004;29:16–22.PubMedGoogle Scholar
  16. Hicks MJ, Flaitz CM, Garcia-Godoy F. Fluoride-releasing sealant and caries-like enamel lesion formation in vitro. J Clin Pediatr Dent. 2000;24:215–9.PubMedGoogle Scholar
  17. Ikemura K, Tay FR, Endo T, Pashley H. A review of chemical-approach and ultramorphological studies on the development of fluoride-releasing dental adhesives comprising new pre-reacted glass ionomer (PRG) fillers. Dent Mater J. 2008;27:315–39.CrossRefPubMedGoogle Scholar
  18. Itota T, Carrick TE, Yoshiyama M, McCabe JF. Fluoride release and recharge in giomer, compomer and resin composite. Dent Mater. 2004;20:789–95.CrossRefPubMedGoogle Scholar
  19. Kantovitz KR, Pascon FM, Correr GM, et al. Influence of environmental conditions on properties of ionomeric and resin sealant materials. J Appl Oral Sci. 2009;17:294–300.PubMedCentralCrossRefPubMedGoogle Scholar
  20. Kavaloglou Cildir S, Sandalli N. Compressive strength, surface roughness, fluoride release and recharge of four new fluoride-releasing fissure sealants. Dent Mater J. 2007;26:335–41.CrossRefGoogle Scholar
  21. Kosior P, Kaczmarek U. Short term fluoride release from conseal F fissure sealant in some media—an in vitro study. Ann Acad Med Stetin. 2006;52:61–5.PubMedGoogle Scholar
  22. Kuşgöz A, Tüzüner T, Ulker M, Kemer B, Saray O. Conversion degree, microhardness, microleakage and fluoride release of different fissure sealants. J Mech Behav Biomed Mater. 2010;3:594–9.CrossRefPubMedGoogle Scholar
  23. Marks D, Owens BM, Johnson WW. Effect of adhesive agent and fissure morphology on the in vitro microleakage and penetrability of pit and fissure sealants. Quintessence Int. 2009;40:763–72.PubMedGoogle Scholar
  24. Mejàre I, Lingström P, Petersson LG, et al. Caries-preventive effect of fissure sealants: a systematic review. Acta Odontol Scand. 2003;61:321–30.CrossRefPubMedGoogle Scholar
  25. Papacchini F, Goracci C, Sadek FT, et al. Microtensile bond strength to ground enamel by glass-ionomers, resin-modified glass-ionomers, and resin composites used as pit and fissure sealants. J Dent. 2005;33:459–67.CrossRefPubMedGoogle Scholar
  26. Rajtboriraks D, Nakornchai S, Bunditsing P, Surarit R, Iemjarern P. Plaque and saliva fluoride levels after placement of fluoride releasing pit and fissure sealants. Pediatr Dent. 2004;26:63–6.PubMedGoogle Scholar
  27. Shen C, Shokry TE, Anusavice KJ. Influence of pH and oxygen-inhibited layer on fluoride release properties of fluoride sealant. J Dent. 2007;35:275–81.CrossRefPubMedGoogle Scholar
  28. Shimazu K, Ogata K, Karibe H. Evaluation of the ion-releasing and recharging abilities of a resin-based fissure sealant containing S-PRG filler. Dent Mater J. 2011;30:923–7.CrossRefPubMedGoogle Scholar
  29. Twetman S, Petersson LG, Axelsson S, et al. Caries preventive effect of sodium chloride mouthrinses: a systematic review of controlled clinical trials. Acta Odontol Scand. 2004;62:223–30.CrossRefPubMedGoogle Scholar
  30. Ulukapi H, Benderli Y, Soyman M. Determination of fluoride release from light-cured glass-ionomers and a fluoridated composite resin from the viewpoint of curing time. J Oral Rehabil. 1996;23:197–201.CrossRefPubMedGoogle Scholar
  31. Wiegand A, Buchalla W, Attin T. Review on fluoride-releasing restorative materials-Fluoride release and uptake characteristics, antibacterial activity and influence on caries formation. Dent Mater. 2007;23:343–62.CrossRefPubMedGoogle Scholar
  32. Williams JA, Billington RW, Pearson GJ. The influence of sample dimensions on fluoride ion release from a glass ionomer restorative cement. Biomaterials. 1999;20:1327–37.CrossRefPubMedGoogle Scholar
  33. Zhou SL, Zhou J, Watanabe S, et al. In vitro study of the effects of fluoride-releasing dental materials on remineralization in an enamel erosion model. J Dent. 2012;40:255–63.CrossRefPubMedGoogle Scholar

Copyright information

© European Academy of Paediatric Dentistry 2015

Authors and Affiliations

  1. 1.Department of Operative Dentistry, School of DentistryAristotle University of ThessalonikiThessalonikiGreece

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