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European Archives of Paediatric Dentistry

, Volume 16, Issue 4, pp 365–370 | Cite as

First-time isolation of Candida dubliniensis from plaque and carious dentine of primary teeth

  • S. KneistEmail author
  • A. Borutta
  • B. W. Sigusch
  • S. Nietzsche
  • H. Küpper
  • M. Kostrzewa
  • A. Callaway
Short communication

Abstract

Aim

To determine those organisms of the genus Candida associated with dental caries by investigating samples from active carious lesions. Within the genus Candida, the species Candida albicans and Candida dubliniensis are capable of forming chlamydospores and germ tubes. Until it became possible in 1995 to differentiate between the two species taxonomically, C. dubliniensis was falsely identified as C. albicans. Whilst the importance of C. albicans for rapidly progressing early childhood caries (ECC) has been recognised, so far there have been only reports about C. dubliniensis in connection with children/mothers who have been infected with HIV or already developed AIDS. In the present study, C. dubliniensis was for the first time isolated from plaque and carious dentine of a healthy five-year-old boy.

Methods

As part of the investigation, a number of samples were collected from individual children affected by active dental caries. Amongst the samples, one in particular indicated that Candida species might be involved. The patient was a five-year-old boy with ECC of the primary dentition, scheduled for restorative treatment under general anaesthesia. Before treatment, a salivary, plaque (region of 54/55) and soft carious dentine sample from the tooth 51 was taken before extraction. The counts of yeasts, lactobacilli (LB) and mutans streptococci were determined in the samples.

Results

The boy’s dmft was 11, which was dominated by the d component. In the saliva of the boy, LB and mutans streptococci (MS) were detected. In plaque and carious dentine, MS and most interestingly C. dubliniensis were present. The yeasts were visualised in carious dentine by means of scanning electron micrographs.

Conclusions

Plaque and carious dentine may be a further habitat of C. dubliniensis.

Keywords

Candida albicans Candida dubliniensis Early childhood caries Caries of primary teeth 

Notes

Conflict of interest

The authors declare that there is no conflict of interest. Dr. M. Kostrzewa is an employee of the company Bruker Daltonik GmbH, Bremen, Germany, the manufacturer of the mass spectrometry system used in this work.

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Copyright information

© European Academy of Paediatric Dentistry 2015

Authors and Affiliations

  • S. Kneist
    • 1
    Email author
  • A. Borutta
    • 2
  • B. W. Sigusch
    • 3
  • S. Nietzsche
    • 4
  • H. Küpper
    • 1
  • M. Kostrzewa
    • 5
  • A. Callaway
    • 6
  1. 1.Biological Laboratory, Clinic for Prosthetic Dentistry and Dental Materials, Centre of DentistryUniversity HospitalJenaGermany
  2. 2.WHO Collaborating Centre on Prevention of Oral Diseases at the Centre for Dental, Oral and Maxillofacial SurgeryUniversity HospitalJenaGermany
  3. 3.Clinic of Conservative Dentistry Centre of DentistryUniversity HospitalJenaGermany
  4. 4.Centre of Electron Microscopy, University HospitalJenaGermany
  5. 5.Bruker Daltonik GmbHBremenGermany
  6. 6.Department of Operative DentistryUniversity Medical CentreMainzGermany

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