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European Archives of Paediatric Dentistry

, Volume 16, Issue 2, pp 219–226 | Cite as

Inhibitory effects of children’s toothpastes on Streptococcus mutans, Streptococcus sanguinis and Lactobacillus acidophilus

  • A. Evans
  • S. J. Leishman
  • L. J. Walsh
  • W. K. SeowEmail author
Original Scientific Article

Abstract

Aim

As suppression of Streptococcus mutans in young children may prevent or delay colonisation of the oral cavity, toothbrushing with dentifrices containing anti-S. mutans activity may aid in preventing caries. The aims of this study were to compare the effects of children’s dentifrices on the growth of S. mutans and non-mutans bacteria (Streptococcus sanguinis and Lactobacillus acidophilus).

Materials and methods

The agar diffusion assay at neutral pH was used to examine the antibacterial activity of commercial dentifrices and their major constituents.

Results

Dentifrices containing 1,450 ppm fluoride produced greater growth inhibition of both S. mutans and S. sanguinis than those with <500 ppm. No inhibition was seen for pure solutions of sodium fluoride or sodium monofluorophosphate at fluoride concentrations up to 100,000 ppm. Stannous fluoride exerted antibacterial effects at concentrations above 10,000 ppm. Significant growth inhibition of both S. mutans and S. sanguinis was seen with sodium lauryl sulphate at 2,500 ppm and with triclosan at 100 ppm. No inhibitory effects were seen for xylitol, sorbitol, sodium pyrophosphate or polyethylene glycol at concentrations up to 80,000 ppm.

Conclusion

Sodium lauryl sulphate is the major bacterial inhibitory compound in children’s dentifrices.

Keywords

Children’s dentifrices Streptococcus mutans Sodium lauryl sulphate Triclosan 

Notes

Acknowledgments

This study was supported in part by the National Health and Medical Research Council of Australia (Grant No.1046779) and the Australian Dental Research Foundation (Grant No. 72-2012). The authors thank Dr. Lei Chai, postdoctoral fellow, School of Dentistry, University of Queensland, for laboratory assistance.

References

  1. Attramadal A, Svatun B. In vivo antibacterial effect of tin on the oral microflora. Scand J Dent Res. 1984;92:161–4.PubMedGoogle Scholar
  2. Australian Research Centre for Population Oral Health. Recommendations for home use of fluoride. 2008. http://www.adelaide.edu.au/arcpoh/dperu/fluoride/Prac_info_Fl_Home_use.pdf. Accessed 9 Sept 2014.
  3. Banas JA, Vickerman MM. Glucan-binding proteins of the oral streptococci. Crit Rev Oral Biol Med. 2003;14:89–99.CrossRefPubMedGoogle Scholar
  4. Blinkhorn A, Bartold PM, Cullinan MP, et al. Is there a role for triclosan/copolymer toothpaste in the management of periodontal disease? Br Dent J. 2009;207:117–25.CrossRefPubMedGoogle Scholar
  5. Block SS. Disinfection, sterilization and preservation. In: Use of metals as microbicides in preventing infections in healthcare. Philadelphia: Lippincott, Williams and Wilkins; 2001.Google Scholar
  6. Buzalaf MAR. Fluoride and the oral environment. Monogr Oral Sci. Basel: Karger; 2011. vol. 22.Google Scholar
  7. Chen F, Rice KC, Liu XM, et al. Triclosan-loaded tooth-binding micelles for prevention and treatment of dental biofilm. Pharm Res. 2010;27:2356–64.CrossRefPubMedCentralPubMedGoogle Scholar
  8. Chikte UM, Rudolph MJ, Reinach SG. Anti-calculus effects of dentifrice containing pyrophosphate compared with control. Clin Prev Dent. 1992;14:29–33.PubMedGoogle Scholar
  9. Ferretti GA, Tanzer JM, Tinanoff N. The effect of fluoride and stannous ions on streptococcus mutans. Viability, growth, acid, glucan production, and adherence. Caries Res. 1982;16:298–307.CrossRefPubMedGoogle Scholar
  10. Gunsolley JC. A meta-analysis of six-month studies of antiplaque and antigingivitis agents. J Am Dent Assoc. 2006;137:1649–57.CrossRefPubMedGoogle Scholar
  11. Hamada S, Torii M, Tsuchitani Y, Kotani S. Isolation and immunobiological classification of Streptococcus sanguis from human tooth surfaces. J Clin Microbiol. 1980;12:243–9.PubMedCentralPubMedGoogle Scholar
  12. Holder IA. In-vitro susceptibility of organisms isolated from burns to topical co-trimoxazole. J Antimicrob Chemother. 1981;7:623–7.CrossRefPubMedGoogle Scholar
  13. Kawabata S, Torii M, Minami T, Fujiwara T, Hamada S. Effects of selected surfactants on purified glycosyltransferases from mutans streptococci and cellular adherence to smooth surfaces. J Med Microbiol. 1993;38:54–60.CrossRefPubMedGoogle Scholar
  14. Koo H. Strategies to enhance the biological effects of fluoride on dental biofilms. Adv Dent Res. 2008;20:17–21.CrossRefPubMedGoogle Scholar
  15. Law V, Seow WK. A longitudinal controlled study of factors associated with mutans streptococci infection and caries lesion initiation in children 21 to 72 months old. Pediatr Dent. 2006;28:58–65.PubMedGoogle Scholar
  16. Lynch RJM, Navada R, Walia R. Low-levels of fluoride in plaque and saliva and their effects on the demineralisation and remineralisation of enamel; role of fluoride toothpastes. Int Dent J. 2004;54:304–9.PubMedGoogle Scholar
  17. Makinen KK. Sugar alcohol sweeteners as alternatives to sugar with special consideration of xylitol. Med Princ Pract. 2011;20:303–20.CrossRefPubMedGoogle Scholar
  18. Marinho VC, Higgins JP, Logan S, Sheiham A. Topical fluoride (toothpastes, mouthrinses, gels or varnishes) for preventing dental caries in children and adolescents. Cochrane Database Syst Rev. 2003:CD002782.Google Scholar
  19. Marquis RE. Antimicrobial actions of fluoride for oral bacteria. Can J Microbiol. 1995;41:955–64.CrossRefPubMedGoogle Scholar
  20. Marquis RE, Clock SA, Mota-Meira M. Fluoride and organic weak acids as modulators of microbial physiology. FEMS Microbiol Rev. 2003;26:493–510.CrossRefPubMedGoogle Scholar
  21. Mayhew RR, Brown LR. Comparative effect of SnF2, NaF, and SnCl2 on the growth of Streptococcus mutans. J Dent Res. 1981;60:1809–14.CrossRefPubMedGoogle Scholar
  22. Mueller JR, Hinton JW. A protein-free medium for primary isolation of Gonococcus and Meningococcus. Proc Soc Exp Biol Med. 1941;48:330–3.CrossRefGoogle Scholar
  23. Murray PR, Zeitinger JR. Evaluation of Mueller–Hinton agar for disk diffusion susceptibility tests. J Clin Microbiol. 1983;18:1269–71.PubMedCentralPubMedGoogle Scholar
  24. Nathan P, Law E, Murphy D. A laboratory method for selection of topical antimicrobial agents to treat infected burn wounds. Burns. 1978;4:177–87.CrossRefGoogle Scholar
  25. Olsson J, Carlen A, Holmberg K. Inhibition of Streptococcus mutans adherence to hydroxyapatite with combinations of alkyl phosphates and nonionic surfactants. Caries Res. 1991;25:51–7.CrossRefPubMedGoogle Scholar
  26. Petersen FC, Assev S, Scheie AA. Combined effects of NaF and sls on acid- and polysaccharide-formation of biofilm and planktonic cells. Arch Oral Biol. 2006;51:665–71.CrossRefPubMedGoogle Scholar
  27. Plonka KA, Pukallus ML, Barnett AG, et al. A longitudinal case-control study of caries development from birth to 36 months. Caries Res. 2013;47:117–27.CrossRefPubMedGoogle Scholar
  28. Rogosa M, Mitchell JA, Wiseman RF. A selective medium for the isolation and enumeration of oral lactobacilli. J Dent Res. 1951;30:682–9.CrossRefPubMedGoogle Scholar
  29. Rykke M, Rolla G, Sonju T. Effect of sodium lauryl sulfate on protein adsorption to hydroxyapatite in vitro and on pellicle formation in vivo. Scand J Dent Res. 1990;98:135–43.PubMedGoogle Scholar
  30. Saxton CA, Svatun B, Lloyd AM. Antiplaque effects and mode of action of a combination of zinc citrate and a nonionic antimicrobial agent. Scand J Dent Res. 1988;96:212–7.PubMedGoogle Scholar
  31. Scheie AA, Petersen FC. Antimicrobials in caries control. In: Fejerskov O, Kidd E, editors. Dental caries the disease and its clinical management. Oxford: Blackwell Munksgaard; 2008. p. 265–77.Google Scholar
  32. Seow WK. The effects of dyadic combinations of endodontic medicaments on microbial growth inhibition. Pediatr Dent. 1990;12:292–7.PubMedGoogle Scholar
  33. Seow WK, Clifford H, Battistutta D, et al. Case-control study of early childhood caries in Australia. Caries Res. 2009;43:25–35.CrossRefPubMedGoogle Scholar
  34. Shimotoyodome A, Koudate T, Kobayashi H, et al. Reduction of Streptococcus mutans adherence and dental biofilm formation by surface treatment with phosphorylated polyethylene glycol. Antimicrob Agents Chemother. 2007;51:3634–41.CrossRefPubMedCentralPubMedGoogle Scholar
  35. Stoodley P, Wefel J, Gieseke A, Debeer D, von Ohle C. Biofilm plaque and hydrodynamic effects on mass transfer, fluoride delivery and caries. J Am Dent Assoc. 2008;139:1182–90.CrossRefPubMedGoogle Scholar
  36. ten Cate JM. Review on fluoride, with special emphasis on calcium fluoride mechanisms in caries prevention. Eur J Oral Sci. 1997;105:461–5.CrossRefPubMedGoogle Scholar
  37. ten Cate JM. Current concepts on the theories of the mechanism of action of fluoride. Acta Odontol Scand. 1999;57:325–9.CrossRefPubMedGoogle Scholar
  38. Tinanoff N, Camosci DA. Microbiological, ultrastructural and spectroscopic analyses of the anti-tooth-plaque properties of fluoride compounds in vitro. Arch Oral Biol. 1980;25:531–43.CrossRefPubMedGoogle Scholar
  39. Tseng CC, Wolff LF, Aeppli DM. Effect of gels containing stannous fluoride on oral bacteria- an in vitro study. Aust Dent J. 1992;37:368–73.CrossRefPubMedGoogle Scholar
  40. Tveit AB, Hals E, Isrenn R, Totdal B. Highly acidic SnF2 and TiF4 solutions. Effect on and chemical reaction with root dentin in vitro. Caries Res. 1983;17:412–8.CrossRefPubMedGoogle Scholar
  41. Van Loveren C. Sugar alcohols: what is the evidence for caries-preventive and caries-therapeutic effects? Caries Res. 2004;38:286–93.CrossRefPubMedGoogle Scholar
  42. Walsh T, Worthington HV, Glenny AM, et al. Fluoride toothpastes of different concentrations for preventing dental caries in children and adolescents. Cochrane Database Syst Rev. 2010:CD007868.Google Scholar
  43. Wong MC, Glenny AM, Tsang BW, et al. Topical fluoride as a cause of dental fluorosis in children. Cochrane Database Syst Rev. 2010:CD007693.Google Scholar
  44. Zameck RL, Tinanoff N. Effects of NaF and SnF2 on growth, acid and glucan production of several oral streptococci. Arch Oral Biol. 1987;32:807–10.CrossRefPubMedGoogle Scholar

Copyright information

© European Academy of Paediatric Dentistry 2014

Authors and Affiliations

  • A. Evans
    • 1
  • S. J. Leishman
    • 1
  • L. J. Walsh
    • 1
  • W. K. Seow
    • 1
    Email author
  1. 1.School of DentistryThe University of QueenslandBrisbaneAustralia

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