Clinical and Translational Imaging

, Volume 1, Issue 6, pp 365–376

Multiagent imaging of the brain

  • Andrea Ciarmiello
  • Chiara Gaeta
  • Claudio Guidotti
  • Massimo del Sette
Review Article

DOI: 10.1007/s40336-013-0042-y

Cite this article as:
Ciarmiello, A., Gaeta, C., Guidotti, C. et al. Clin Transl Imaging (2013) 1: 365. doi:10.1007/s40336-013-0042-y


Positron emission tomography (PET) and single-photon emission computed tomography (SPECT) are in vivo imaging techniques that, using a wide range of tracers, allow the non-invasive tracking of pathophysiological processes in healthy and diseased brain. One of most promising of the various PET and SPECT applications is the investigation of pathophysiological aspects of neurodegenerative disorders. This is an extremely important area of investigation given the aging of the global population and the high prevalence of brain disorders such as Alzheimer’s disease and Parkinson’s disease in elderly persons. Clinical translation of advances in molecular imaging research into clinical practice may, by overcoming the limitations of a diagnostic approach that relies exclusively on clinical judgment and structural imaging, lead to better clinical management of affected patients.


Brain Neurodegenerative diseases Molecular imaging PET SPECT PET/MRI 


Positron emission tomography (PET) and single-photon emission computed tomography (SPECT) are in vivo imaging techniques that allow the non-invasive tracking of pathophysiological processes in healthy and diseased brain. The unique feature of in vivo emission tomography techniques is their potential for allowing integration of information about neuronal function, viability, neurotransmission systems, along with protein deposition and immune system activation. This integration of data about cerebral (dys)functions at cellular and molecular level is possible using a multiagent imaging approach.

Indeed, the use of an imaging approach that exploits the wide range of radiotracers developed in recent decades will likely lead to a better understanding of the pathophysiology of brain disorders, of their natural course, and consequently of their possible treatments.

In research settings, PET and SPECT radiotracers have been successfully used in drug development, for further understanding of the mechanism of action of pharmacological agents in the central nervous system [1]. In daily clinical practice, combining different tracers and techniques in sequential imaging sessions constitutes the current diagnostic workup for several brain diseases.

One of most promising of the various PET and SPECT applications is the investigation of pathophysiological aspects of neurodegenerative disorders. This is an extremely important area of investigation given the aging of the global population and the high prevalence of brain disorders such as Alzheimer’s disease (AD) and Parkinson’s disease (PD) in elderly life.

This review will focus on the perspectives for PET and SPECT radiotracers capable of mapping intracellular and extracellular mechanisms underlying several brain disorders that have a particularly high impact both on individuals and on society. Our discussion will deal mainly with neurodegenerative disorders.


Dementia is a term used to refer to a variety of neurodegenerative disorders associated with considerable disability and high costs to individuals and society [2]. The most common neurodegenerative disorders responsible for dementia, such as AD, dementia with Lewy bodies (DLB) and frontotemporal dementia (FTD), have different underlying pathogenetic mechanisms; each is associated with a distinctive course and its own set of possible complications [3].

A diagnosis of dementia is currently made in terms of probability and is based on clinical features as well as on the results of structural brain imaging investigations [computed tomography (CT) or magnetic resonance imaging (MRI)] and blood tests [4, 5].

However, the accuracy of these diagnostic criteria is limited compared with post-mortem histopathology [6].

Even though symptomatic medications are the only treatments currently available, an accurate diagnosis of dementia is critically important to avoid inappropriate interventions and to provide guidance for appropriate non-pharmacological management.

The recent introduction, in both investigational and clinical settings, of imaging biomarkers as an optional tool for AD diagnosis represents a cultural shift towards a more biologically focused workup in dementia [7, 8]. Clinical translation of the advances in molecular imaging research may, by allowing more accurate diagnosis of dementia, lead to better clinical management of affected patients. Moreover, such biomarkers may also make it possible to achieve early etiological characterization of the prodromal stage of dementia, also known as mild cognitive impairment (MCI) [9]. This is a particularly important aspect, given that the disease-modifying drugs currently under development are expected to be more effective in the early disease phase [10].

Neuronal damage or dysfunction and specific pathological features of dementias are the targets of the most widely validated PET biomarkers [11].

Neurodegeneration assessed using [18F]FDG PET has been extensively evaluated in dementia patients [12]. In fact, the cerebral metabolic rate of glucose (CMRglc) reflects synaptic activity and density [13]. Metabolic deficits in AD patients are present in the neocortical association areas, with reductions detected predominantly in temporoparietal regions, the posterior cingulate cortex and the frontal cortex [14] (Fig. 1). Metabolic reductions in the medial occipital cortex are typically observed in DLB, whereas metabolic impairments in FTD typically involve frontal or frontotemporal regions [15] (Fig. 1). [18F]FDG PET has been shown to correctly differentiate AD subjects from healthy controls with high sensitivity and specificity, using either clinical assessment or pathological confirmation as reference standard [16, 17, 18, 19, 20]. In addition, despite some overlap in the hypometabolic patterns, several studies have proved the feasibility of using [18F]FDG PET in the differential diagnosis of the major neurodegenerative disorders, the technique being found to show higher diagnostic accuracy than clinical judgment [21].
Fig. 1

Images showing normal [18F]FDG distribution in a healthy subject (a), and reduced uptake in posterior brain areas in an MCI subject (b) and a DLB subject (c). This reduced uptake is particularly evident in the occipital areas of the DLB patient (color figure online)

The added value of [18F]FDG PET over the clinical assessment of patients suspected of having AD was shown by Jagust et al. [22]. The probability of detecting post-mortem AD pathology increases from 70 to 84 % in a patient with a positive PET scan, whereas it decreases to 31 % in the presence of a negative scan. Conversely, a negative clinical evaluation for AD is associated with a 35 % probability of post-mortem pathology, versus 70 % in the case of a positive [18F]FDG PET scan.

CMRglc reductions in patients with MCI predict progression to AD with high accuracy [23, 24] and have been found to be highly correlated with the severity of the clinical impairment [25]. Even though the hypometabolic pattern in patients with DLB can be similar to that observed in AD, the involvement of the occipital cortices is usually more severe while the temporal lobes are less affected [26].

β-amyloid (Aβ) plaques are present in the cortical gray matter in all cases of AD, in 50–70 % of patients with DLB, and in normal elderly people in a percentage that increases with age [27]. Since the advent of the first amyloid tracer, 11C-labeled Pittsburgh compound B (PiB) [27], several studies have been published assessing the amyloid status of individuals with dementia.

In the attempt to overcome the limitations related to the short half-life of 11C (20 min), 18F-labeled Aβ radiopharmaceuticals have been developed, including 18F-3′-F-PiB (flutemetamol), 18F-AV-45 (florbetapir), 18F-AV-1 or 18F-BAY94-9172 (florbetaben), and 18F-AZD4694 or NAV4694. A high correlation between PiB and 18F-labeled ligands has been demonstrated [27], supporting the translation of PiB PET findings into the domain of 18F-labeled radiopharmaceuticals [28].

PiB PET studies have shown a robust difference in PiB uptake between AD patients and age-matched controls, reporting very high sensitivity (90 %) and high negative predictive value. On the other hand, specificity and positive predictive value decline with age, showing a diagnostic accuracy for AD of 75–80 % in patients over 80 years of age [11]. However, if the purpose of the diagnostic assessment is to evaluate the presence of Aβ plaques, the accuracy of amyloid imaging remains high at all ages [27]. A positive amyloid PET does not establish a diagnosis of AD, but it can help to support or exclude the presence of AD in the context of a more comprehensive evaluation of the patient [28].

An important clinical application of amyloid PET is the differential diagnosis between AD and FTD, as Aβ pathology is not present in the latter. In a recent study [29] that included 62 AD patients and 45 FTD patients, PiB and [18F]FDG PET showed similar diagnostic accuracy. However, PiB was more sensitive and showed higher inter-rater reliability and agreement between qualitative and quantitative evaluation.

Since the publication of the amyloid cascade hypothesis [30], according to which accumulation of Aβ during early stages of the disease precedes a cascade of neuropathological events that eventually lead to AD, intense efforts have been directed at evaluating the possible role of amyloid imaging in the prognostic assessment of MCI patients and cognitively normal elderly people. Even though several studies support the notion that many healthy elderly people with a positive PiB PET are in a preclinical phase of AD, this hypothesis requires further longitudinal investigation [31]. On the other hand, current data suggest that amyloid PET may correctly stratify MCI patients, detecting those who will convert to AD [32]. Forsberg et al. [33] showed that the mean cortical PiB retention in MCI patients was intermediate compared with the value recorded in healthy controls and AD patients. MCI patients who converted to AD during follow-up compared to non-converting patients had a significantly higher PiB retention, comparable to that of AD patients.

The findings of several longitudinal studies, which set out to compare PiB retention, [18F]FDG uptake and cognitive impairment in AD and MCI patients, suggest a bidirectional interrelationship between neurodegeneration assessed by [18F]FDG PET and Aβ deposition evaluated by PiB PET [34]. Amyloid-positive regions show maintenance of neuronal activity in an early preclinical phase, followed by progressive neuronal degeneration coupled with cognitive decline. In addition, while neuronal degeneration and cognitive impairment continue in AD patients, the accumulation of Aβ appears to be stable. Thus, whereas PiB studies show inverse correlations with glucose hypometabolism in some brain regions in AD patients, no significant correlations are observed in MCI patients. Accordingly, PiB PET does not correlate with cognitive impairment in AD patients, whereas this correlation is observed in MCI patients [27]. In conclusion, whereas [18F]FDG PET can be used to determine the degree and progression of neurodegeneration [35], amyloid PET appears to be a powerful tool for the early diagnosis of AD pathology that may lead to a greater level of diagnostic confidence in particular cases, such as MCI patients, patients with possible AD and atypical clinical presentation, or patients with atypically young-onset dementia [28].

In DLB, amyloid deposits are associated with AD-like atrophy, predominantly involving the parahippocampal area and lateral temporal and parietal cortices [36]. In a study comparing DLB patients with patients affected by Parkinson’s disease dementia (PDD), the presence of amyloid plaques in the DLB patients was found to be linked to the cognitive impairment and to the timing of the onset of dementia symptoms relative to parkinsonism [37].

Neurofibrillary tangles (NFTs) result from the abnormal aggregation of tau protein and can be regarded as another pathological hallmark of AD [38]. Among several probes developed to detect tau pathology in the brain, the radiotracer FDDNP (i.e., 2-(1-{6-[(2-[18F]fluoroethyl)(methyl)amino]-2-naphthyl}ethylidene)malononitrile) has been shown to label senile plaques and NFTs in vitro [39]. PET with FDDNP has proved able to distinguish between cognitively normal, MCI and AD subjects [40]. More recently, FDDNP has been proposed as a feasible diagnostic tool for stratifying non-demented subjects with different levels of dementia risk [41].

Inflammatory mechanisms, like microglial activation, are thought be involved in the pathogenesis of AD. Furthermore, pathological and animal studies have demonstrated that microglia are attracted to sites of amyloid deposition. However, the first in vivo assessments of microglial activation using PET and 11C-PK11195 (a marker of translocator protein) in AD and DLB have given discordant results. Okello et al. [42] showed increased binding of 11C-PK11195 in the frontal cortex in a small group of PiB-positive versus PiB-negative MCI patients. Neuroinflammation was also reported in DLB patients compared with controls [43]. Conversely, a more recent study comparing patients with probable AD, MCI patients and healthy controls did not demonstrate significant differences in uptake between the groups [44]. Larger studies and a better understanding of the role of inflammation in dementia are needed to draw definite conclusions.

Neurotransmitter systems (i.e. the cholinergic, dopaminergic, GABAergic and serotonergic systems) are impaired in dementia. A wide range of PET and SPECT radiotracers is available for studying the integrity of these systems.

Over the years, several SPECT and PET studies using [123I]IBVM ([123I]iodobenzovesamicol), a pre-synaptic ligand for the acetylcholine (ACh) vesicular transporter, and [11C]MP4A (N-[11C]methylpiperidin-4-yl acetate), an ACh analog whose trapping in the brain is directly proportional to the presence of acetylcholinesterase (AChE), have demonstrated the occurrence of a cortical pre-synaptic cholinergic decline mainly in severe–moderate AD and in early-onset cases of AD [45, 46]. A marked reduction in cortical AChE was demonstrated using PET with [11C]MP4A in patients with DLB, in whom the phenomenon was found to be more severe than in AD patients [47].

The density of pre-synaptic membrane dopamine transporters (DATs) and of dopamine terminal vesicular monoamine transporters can be assessed using various SPECT/PET radiotracers. DAT imaging with 123I-FP-CIT SPECT is useful for diagnosing DLB and exhibits sufficient diagnostic accuracy to distinguish DLB from AD [48]. An abnormal DAT scan in a patient with a clinical suspicion of DLB or other dementia shows a diagnostic sensitivity of 83 % and specificity of 100 % for autopsy-confirmed DLB [49].

Recently, a combined PET imaging approach with 11C-dihydrotetrazine, a striatal vesicular monoamine transporter marker, and PiB has been proposed as diagnostic stratification tool to aid in differential diagnosis of AD, DLB and FTD [50]. Alterations in the GABAergic system can be evaluated assessing the availability of GABA receptors using 11C-flumazenil PET or 123I-iomazenil SPECT. Even though cortical depletion of GABA receptors has been proven by post-mortem studies in AD, in vivo imaging studies with PET or SPECT provided discordant results [51, 52].

Brain perfusion imaging with the two most commonly employed SPECT tracers, 99mTc-hexamethylpropylenamine (HMPAO) and 99mTc-ethylcysteinate dimmer (ECD), has been extensively used in the diagnosis, therapeutic management and follow-up of dementia patients [53]. In the absence of cerebrovascular disease, hypoperfusion is related to impaired neuronal activity and is coupled with decreased metabolism detected by [18F]FDG PET, showing similar patterns of reduced regional uptake in different types of dementia. However, correlations of abnormal tracer uptake between [18F]FDG PET and HMPAO SPECT in the same subjects are only modest (r = 0.43), with the best correspondence being found in the temporoparietal and posterior cingulate association cortices [54]. [18F]FDG PET performs better in discriminating between AD patients and controls, showing a sensitivity of 100 % compared to the 90 % of SPECT [55].

Thus, when it is possible to choose between PET and SPECT, PET should be preferred. Nevertheless, SPECT can be considered a reliable alternative.

Movement disorders

PD is a progressive neurodegenerative disease clinically characterized by rigidity, rest tremors and bradykinesia associated with the loss of dopaminergic neurons in the substantia nigra pars compacta and a subsequent striatal dopaminergic deficiency [56]. Even though the U.K. Parkinson’s Disease Society Brain Bank diagnostic criteria have improved the accuracy of clinical diagnosis of PD, anatomical-pathological studies nevertheless show a high rate of misdiagnosis (25 %) [57]. Furthermore, when diagnosis is based entirely on clinical criteria, treatment may be delayed for years until functional disability appears. Molecular imaging with SPECT or PET allows a more accurate diagnostic workup and can be used for the differential diagnosis between PD and parkinsonian syndromes (also known as Parkinson-plus syndromes) such as multiple system atrophy (MSA), progressive supranuclear palsy (PSP), corticobasal degeneration (CBD) and diffuse Lewy body disease (DLBD).

The availability of pre-synaptic DATs can be assessed using a variety of SPECT and PET tracers, including 123I-β-CIT, 123I-FP-CIT (DaTSCAN), 123I-altropane, 99mTc-TRODAT-1 and 11C-CFT ([11C]2β-carbomethoxy-3β-(4-fluorophenyl)tropane) [58]. Other pre-synaptic PET tracers such as 18F-DOPA and 11C- or 18F-dihydrotetrabenazine (DTBZ) are markers, respectively, of terminal DOPA aromatic acid decarboxylase activity (AADC) and of type 2 vesicle monoamine transporter availability [59].

Over the years, various studies using SPECT with 123I-b-CIT and 123I-FP-CIT showed a clear reduction of striatal DATs in patients with PD [60, 61]. Differential diagnosis between PD and other parkinsonian syndromes from those with essential tremor or other neurological diseases which do not involve the nigrostriatal dopaminergic system can be particularly challenging when based exclusively on clinical features. Compared with clinical assessment, 123I-FP-CIT SPECT was found to show higher specificity in differentiating between PD and essential tremor [61] (Fig. 2). The impact on clinical management can be considered one of the most direct measures of the benefit derived from a diagnostic imaging modality. The results of two studies including patients with clinically uncertain parkinsonism syndromes have shown that including a measure of striatal dopaminergic function in the workup of uncertain parkinsonism cases helps in planning their management. In fact, when 123I-FP-CIT SPECT findings were divulged to clinicians, the diagnosis of parkinsonian syndrome was revised in 52 % of the 118 patients and the management strategy was changed in 72 % [62]. Also, 123I-FP-CIT SPECT was found to have a high negative predictive value. Only 3 % of 150 patients suspected of having a parkinsonian syndrome but presenting normal dopamine terminal function on SPECT showed clinical progression during 2 years of follow-up [63]. DAT imaging may be useful in identifying individuals with early PD (i.e. before onset of symptoms) showing a relative reduction in uptake in the putamen compared with the caudate [64].
Fig. 2

Normal distribution of 123I-FP-CIT (DaTSCAN) in a healthy subject (a) and in an essential tremor subject (b). In a PD patient (c), the right putamen shows a reduced concentration of radiotracer (color figure online)

Various studies have demonstrated the feasibility of using 18F-DOPA PET to differentiate PD patients from healthy controls. Jokinen et al. [65] showed a lower striatal-to-occipital ratio in PD patients compared with controls, with the lowest ratio being found in the posterior putamen contralateral to the side of predominant clinical symptoms. However, because of up-regulation of AADC activity in the surviving cells, 18F-DOPA may underestimate the severity of nigrostriatal loss, especially in the early stages of disease [66].

11C- or 18F-DTBZ uptake is decreased in the corpus striatum in PD patients compared with control subjects and it is not affected by synaptic dopamine levels or dopaminergic agents [67]. Thus, even though its use has, to date, been limited to research settings, DTBZ can be considered one of the best available ligands for examining dopaminergic system integrity [68].

As pre-synaptic dopaminergic nerve terminals are involved in most degenerative parkinsonian disorders, pre-synaptic dopaminergic imaging performs poorly in differentiating between PD and Parkinson-plus syndromes. Conversely, this differential diagnosis is possible using post-synaptic dopaminergic imaging assessing post-synaptic D2 receptor density with benzamide derivative ligands, such as 11C-raclopride and 123I-IBZM (123I-iodobenzamide). In fact, various authors have shown reduced striatal binding of D2 receptor ligands in patients with Parkinson-plus syndromes and increased binding in untreated patients with early PD [69, 70, 71]. Even though international guidelines recommend post-synaptic imaging to distinguish PD from Parkinson-plus syndromes [69], a recent meta-analysis on the role of SPECT in parkinsonian syndromes indicated a low level of diagnostic accuracy in differentiating between PD and Parkinson-plus syndromes [72]. Furthermore, post-synaptic dopaminergic imaging does not discriminate among Parkinson-plus syndrome subgroups. For this reason, different tracer approaches have been proposed.

It has been demonstrated that [18F]FDG PET can reveal regional differences in glucose metabolism between PD and Parkinson-plus syndromes [73]. However, broad application of metabolic imaging in these neurodegenerative diseases has been limited on account of the substantial variability in brain activity between subjects and brain regions. To overcome these limitations, a “network” analysis approach was proposed, based on the assessment of functional interactions between brain regions [74]. The rationale for this approach was that neurodegenerative processes, even if highly localized, are associated with disease-specific alterations in functional connectivity across the whole brain. Applying this method of analysis, PD appears to be characterized by increased pallidothalamic and pontine metabolic activity and relative reductions in activity in the premotor cortex, supplemental motor area, and parietal association regions [75]. MSA shows metabolic decreases in the putamen and the cerebellum, whereas PSP shows metabolic decreases predominantly in the upper brainstem and medial prefrontal cortex, as well as in the medial thalamus, the caudate nuclei, the anterior cingulate area, and the superior frontal cortex. Asymmetrical decreases in FDG uptake in frontal and parietal cortices and subcortical regions have been reported in CBD patients, whereas DLBD is characterized by bilateral metabolic reduction in the occipital cortex [76].

Lewy bodies are constantly present in the sympathetic ganglia of PD and DLBD patients [77], leading to degeneration of adrenergic post-ganglionic pathways, a phenomenon that can be assessed using specific ligands targeting the adrenergic terminations at cardiac level. PET with 11C-MHED or 18F-DOPA and SPECT with 123I-MIBG showed reduced cardiac uptake in PD and DLBD patients compared with patients with other Parkinson-plus syndromes [78, 79].

Microglial activation has been associated with loss of substantia nigra neurons in PD and appears to be widespread in the end stage of the disease, being detected histochemically in the basal ganglia, cingulate, hippocampus and cortical association areas [80]. PET studies in PD patients using 11C-PK11195 showed an increased midbrain uptake, inversely correlated with the uptake of 11C-CFT. Other authors demonstrated increased 11CPK11195 uptake in the medulla and pons, striatum, pallidum and frontal cortex, a pattern that reflects the distribution of Lewy body pathology [81]. The results of a 2-year follow-up study of PD patients suggest that microglial activation does not change during the disease course, even in the presence of a clinical deterioration [81]. These findings suggest that early treatment with neuroprotective drugs to suppress microglial activation could have favorable effects in PD patients. In MSA, significant 11C-PK11195 uptake was observed in the putamen, pallidum, pons, substantia nigra pars compacta, and dorsolateral prefrontal cortex, in topographic correspondence with the distribution of neuropathological alterations [82]. In PSP patients, 11C-PK11195 PET showed significant levels of activated microglia in the midbrain, cerebellum, pons, frontal lobe, and basal ganglia, while in CDB patients activated microglia were observed at the level of the caudate nucleus, putamen, substantia nigra pars compacta, pons, and pre- and postcentral gyrus [81, 83].

A significant number of PD patients develop either MCI or frank dementia, having a sixfold higher risk of developing dementia compared with healthy controls [84]. The pathological basis of dementia in PD is likely to be multifactorial. Possibilities include cortical Lewy body disease and concomitant Aβ plaque deposition, among others. It is still unclear whether PDD and DLB are part of a spectrum or separate disease entities [84]. DLB is clinically distinguished from PDD purely based on the different timing of onset of dementia symptoms in the two conditions. Histopathology analyses show that cortical Aβ deposition can be found in PDD, albeit less frequently than in DLB [85]. Several studies using 11C-PiB PET to compare DLB, PDD and PD without dementia show an increased uptake in only a minority of PDD cases, in whom the pattern of distribution of Aβ plaques is similar to that observed in DLB [86, 87]. Even though findings regarding the association between Aβ plaque load and cognitive decline in PDD and DLB are discordant, in vivo imaging markers of amyloid load may be able to identify those PDD and DLB subjects who can benefit from novel anti-amyloid strategies.

Huntington’s disease (HD) is an autosomal dominant neurodegenerative disease caused by an expansion mutation of the CAG gene over 35 trinucleotide repeats in the Huntington’s gene on chromosome 4p16.3 [88, 89]. The number of CAG repeats is a major determinant of the age at onset of HD and other poly-(CAG) diseases. Symptoms are characterized by the triad of cognitive impairment, psychiatric disturbance and movement disorder. The pathology preferentially affects striatal areas and the caudate nucleus appears to be more severely affected than the putamen. The mechanism leading to neuronal death is not completely understood, but it is believed that abnormal huntingtin may be noxious to neurons through glutamate-mediated excitotoxicity [90]. Screening genetic tests allow identification of subjects at risk of the disease. All patients showing triplet abnormalities will eventually develop clinically evident disease. Age at onset of disease can be predicted using regression analysis based on the number of triplets. However, the penetrance of the genetic abnormality is variable and there is considerable variability in the age at onset of disease even in patients with similar number of triplets [91].

[18F]FDG PET studies in symptomatic HD patients revealed reduced striatal glucose metabolism. However, in the early symptomatic phases of HD, only caudate hypometabolism has been reliably identified, and putamen metabolism may be normal [92]. In symptomatic patients, as well as in presymptomatic (preHD) subjects that are healthy carriers of the CAG mutation, a significant correlation was found between striatal metabolism and the estimated time to symptom onset (Fig. 3) [93, 94]. Interestingly, both in clinically unaffected subjects at risk of HD and in symptomatic patients, metabolic reductions were not only confined to the striatum but also involved the frontal cortex and the temporal lobes. The metabolic decline correlated linearly with the severity of motor symptoms calculated using the Unified Huntington Disease Rating Scale (UHDRS). Similarly, the rate of metabolic changes in the frontal and temporal areas of the cerebral cortex correlated linearly with worsening behavioral scores on UHDRS-III. Application of brain tissue segmentation techniques to PET data to correct for partial volume effect showed that metabolic reductions were not artifacts due to brain atrophy since CMRglu deficits remained significant even after partial volume correction. These results highlight that HD affects the cerebral cortex in addition to the striatum, and that the disease is associated with loss of white as well as of gray matter [93, 94].
Fig. 3

Linear dependence of predicted age at onset on a CAG-based model and a multivariate model (CAG-based + striatum [18F]FDG uptake). Regression line and 95 % confidence interval between true age at disease onset and estimated age at disease onset by the regression model including CAG expansion alone (a) and CAG expansion combined with glucose metabolism in the striatum (b) in 26 phenoconverted subjects. Regression equations were y = 14 + 0.63x, R2 = 0.63, p < 0.0001 (a), and y = −4.19e−13 × 1x, R2 = 0.86, p < 0.0001 (b)

Many studies showing early defects in cortical and striatal glucose metabolism [93, 95] have driven the search for sensitive brain markers (dry biomarkers) that might be measured before HD becomes manifest, thereby making it possible to predict how quickly overt symptoms are likely to appear in at-risk mutation carriers. Research models to date attempting to predict the age at onset in unaffected mutation carriers have merely sought to relate CAG expansion size to subject age [96]. Recently a new model for predicting the age at onset was reported: this model includes, along with CAG expansion and the subject’s age, uptake of [18F]FDG on PET. The study group comprised 43 preHD subjects carrying the HD mutation. They underwent an [18F]FDG PET scan and were prospectively followed up for at least 5 years using the UHDRS to detect clinical changes. The authors showed that caudate hypometabolism detected in the brains of HD subjects precedes early clinical phenoconversion. This new finding should help to improve prediction of age at onset in HD [97].

PET with [18F]FDG has been used for monitoring HD patients that are being treated with riluzole, a glutamate receptor antagonist that is thought to reduce excitotoxicity. Patients treated with riluzole showed significantly less gray matter volume loss and less severe CMRglc decreases in the striatum and in cortical areas. Moreover, plasma levels of brain-derived neurotrophic factor were significantly higher in patients treated with riluzole [94].

In a recent study by Politis et al., MRI and PET were combined to investigate the functional and structural changes of the brain in asymptomatic mutation carriers. Regional volume changes were studied using MRI volumetric analysis, whereas 11C-raclopride and 11C-PK11195 PET were used to investigate, respectively, the integrity of post-synaptic dopamine D2/D3 receptors and the presence of altered levels of activated microglia. The authors found reduced brain volume and reduced 11C-raclopride specific binding. Interestingly, significantly increased 11C-PK11195 uptake was found in sensorimotor and associative striatum of the preHD cases, leading the authors to conclude that activated microglia in brain areas related to cognitive function may be a predictor of clinical onset [98].

In vitro studies showed that neuronal loss in HD is associated with derangements in many neurotransmitter systems. The role of dopamine in the clinical manifestations of HD has been a topic of particular interest given the prominence of the movement disorder in this condition. Changes in the dopaminergic system have been assessed in HD patients using PET. Striatal binding correlated negatively with disease stage and positively with the Mini-Mental State Examination score. Both D1 and D2 receptor binding were significantly reduced in the moderate-advanced patients and in the mutation carriers [99]. Patients with more rigidity showed greater receptor loss than patients without significant rigidity [100]. A decrease in D1 and D2 receptors has also been reported in asymptomatic mutation carriers [101].

Another area of interest is the gabaergic system. The vast majority (>90 %) of striatal neurons are gabaergic. Abnormalities in the gabaergic system were investigated using PET and 11C-flumazenil, an antagonist of the central benzodiazepine receptor [102]. It was found that benzodiazepine receptor density was significantly decreased in the caudate nucleus of HD patients compared with controls. This abnormality was restricted to the striatum even though glucose metabolism was significantly reduced not only in the caudate nucleus but also in the putamen and thalamus [102].

Molecular neuroimaging with PET/MRI

PET/MRI is a new hybrid imaging technology which is attracting growing interest in the medical community because of its potential clinical and research applications. The availability of new MRI techniques and sequences such as MR spectroscopy, diffusion-weighted imaging (DWI), dynamic contrast-enhanced MRI (DCE-MRI) and blood oxygen level-dependent (BOLD) fMRI has broadened the diagnostic spectrum of MRI beyond anatomical morphological imaging of the brain. Indeed, MR spectroscopy provides a measure of biochemicals in tissue, DWI assesses cellular density, tissue architecture and extracellular space tortuosity, DCE-MRI describes vessel density, vascular permeability and tissue perfusion, while BOLD fMRI evaluates changes in blood oxygen levels.

By providing co-registered functional PET and anatomical/functional MR images, PET/MRI opens up exciting possibilities for the evaluation of brain diseases. All the new MRI techniques and sequences can potentially be used in combination with the molecular information provided by PET [103].


Currently, several tracers for PET and SPECT imaging are widely used in the diagnostic workup of patients with brain diseases. Many other tracers have recently been developed and may lead to better clinical management of patients, overcoming the limitations of a diagnostic approach that relies exclusively on clinical judgment and structural imaging. Molecular imaging, providing a better understanding of pathophysiological processes underlying these disorders, opens up exciting new possibilities for the development of treatments aimed at modifying the natural course of different brain diseases.

Conflict of interest

The authors of the manuscript—Andrea Ciarmiello, Chiara Gaeta, Claudio Guidotti and Massimo del Sette—declare no conflict of interest.

Compliance with ethics guidelines

This article does not contain any study with human or animal subjects performed by any of the authors.

Copyright information

© Italian Association of Nuclear Medicine and Molecular Imaging 2013

Authors and Affiliations

  • Andrea Ciarmiello
    • 1
  • Chiara Gaeta
    • 1
  • Claudio Guidotti
    • 1
  • Massimo del Sette
    • 2
  1. 1.Department of Nuclear MedicineS. Andrea HospitalLa SpeziaItaly
  2. 2.Neurology DepartmentS. Andrea HospitalLa SpeziaItaly

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