Journal of Arid Land

, Volume 6, Issue 4, pp 410–422 | Cite as

Response of soil N2O emissions to precipitation pulses under different nitrogen availabilities in a semiarid temperate steppe of Inner Mongolia, China

  • XinChao Liu
  • YuChun Qi
  • YunShe DongEmail author
  • Qin Peng
  • YaTing He
  • LiangJie Sun
  • JunQiang Jia
  • CongCong Cao


Short-term nitrous oxide (N2O) pulse emissions caused by precipitation account for a considerable portion of the annual N2O emissions and are greatly influenced by soil nitrogen (N) dynamics. However, in Chinese semiarid temperate steppes, the response of N2O emissions to the coupling changes of precipitation and soil N availability is not yet fully understood. In this study, we conducted two 7-day field experiments in a semiarid temperate typical steppe of Inner Mongolia, China, to investigate the N2O emission pulses resulting from artificial precipitation events (approximately equivalent to 10.0 mm rainfall) under four N addition levels (0, 5, 10, and 20 g N/(m2·a)) using the static opaque chamber technique. The results show that the simulated rainfall during the dry period in 2010 caused greater short-term emission bursts than that during the relatively rainy observation period in 2011 (P<0.05). No significant increase was observed for either the N2O peak effluxes or the weekly cumulative emissions (P>0.05) with single water addition. The peak values of N2O efflux increased with the increasing N input. Only the treatments with water and medium (WN10) or high N addition (WN20) significantly increased the cumulative N2O emissions (P<0.01) in both experimental periods. Under drought condition, the variations in soil N2O effluxes were positively correlated with the soil NH4-N concentrations in the three N input treatments (WN5, WN10, and WN20). Besides, the soil moisture and temperature also greatly influenced the N2O pulse emissions, particularly the N2O pulse under the relatively rainy soil condition or in the treatments without N addition (ZN and ZWN). The responses of the plant metabolism to the varying precipitation distribution and the length of drought period prior to rainfall could greatly affect the soil N dynamics and N2O emission pulses in semiarid grasslands.


temperate semiarid steppe nitrous oxide nitrogen availability precipitation 


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  1. Austin A T, Yahdjian L, Stark J, et al. 2004. Water pulses and biogeochemical cycles in arid and semiarid ecosystems. Oecologia, 141(2): 221–235.CrossRefGoogle Scholar
  2. Austin A T. 2011. Has water limited our imagination for aridland biogeochemistry? Trends in Ecology & Evolution, 26(5): 229–235.CrossRefGoogle Scholar
  3. Barton L, Kiese R, Gatter D, et al. 2008. Nitrous oxide emissions from a cropped soil in a semi-arid climate. Global Change Biology, 14(1): 177–192.Google Scholar
  4. Birch H F. 1964. Mineralisation of plant nitrogen following alternate wet and dry conditions. Plant and Soil, 20(1): 43–49.CrossRefGoogle Scholar
  5. Borken W, Matzner E. 2009. Reappraisal of drying and wetting effects on C and N mineralization and fluxes in soils. Global Change Biology, 15(4): 808–824.CrossRefGoogle Scholar
  6. Burger M, Jackson L E, Lundquist E J, et al. 2005. Microbial responses and nitrous oxide emissions during wetting and drying of organically and conventionally managed soil under tomatoes. Biology and Fertility of Soils, 42(2): 109–118.CrossRefGoogle Scholar
  7. Cantarel A, Bloor J, Deltroy N, et al. 2011. Effects of climate change drivers on nitrous oxide fluxes in an upland temperate grassland. Ecosystems, 14(2): 223–233.CrossRefGoogle Scholar
  8. Collins S L, Sinsabaugh R L, Crenshaw C, et al. 2008. Pulse dynamics and microbial processes in aridland ecosystems. Journal of Ecology, 96(3): 413–420.CrossRefGoogle Scholar
  9. Davidson E A. 1992a. Sources of nitric oxide and nitrous oxide following wetting of dry soil. Science Society of America Journal, 56(1): 95–102.CrossRefGoogle Scholar
  10. Davidson E A. 1992b. Pulses of nitric oxide and nitrous oxide flux following wetting of dry soil: an assessment of probable sources and importance relative to annual fluxes. Ecological Bulletins, 42: 149–155.Google Scholar
  11. Davidson E A, Matson P A, Vitousek P M, et al. 1993. Processes regulating soil emissions of NO and N2O in a seasonally dry tropical forest. Ecology, 74(1): 130–139.CrossRefGoogle Scholar
  12. Dijkstra F A, Augustine D J, Brewer P, et al. 2012. Nitrogen cycling and water pulses in semiarid grasslands: are microbial and plant processes temporally asynchronous? Oecologia, 170(3): 799–808.CrossRefGoogle Scholar
  13. Dobbie K E, Smith K A. 2003. Nitrous oxide emission factors for agricultural soils in Great Britain: the impact of soil water-filled pore space and other controlling variables. Global Change Biology, 9(2): 204–218.CrossRefGoogle Scholar
  14. Dong Y S, Zhang S, Qi Y C, et al. 2000. Fluxes of CO2, N2O and CH4 from a typical temperate grassland in Inner Mongolia and its daily variation. Chinese Science Bulletin, 45(17): 1590–1594.CrossRefGoogle Scholar
  15. Du R, Lu D R, Wang G C. 2006. Diurnal, seasonal, and inter-annual variations of N2O fluxes from native semi-arid grassland soils of inner Mongolia. Soil Biology and Biochemistry, 38(12): 3474–3482.CrossRefGoogle Scholar
  16. Fan J W, Zhong H P, Harris W, et al. 2008. Carbon storage in the grasslands of China based on field measurements of above- and below-ground biomass. Climatic Change, 86(3–4): 375–396.CrossRefGoogle Scholar
  17. Fierer N, Schimel J P. 2002. Effects of drying-rewetting frequency on soil carbon and nitrogen transformations. Soil Biology and Biochemistry, 34(6): 777–787.CrossRefGoogle Scholar
  18. Freibauer A, Kaltschmitt M. 2003. Controls and models for estimating direct nitrous oxide emissions from temperate and sub-boreal agricultural mineral soils in Europe. Biogeochemistry, 63(1): 93–115.CrossRefGoogle Scholar
  19. Goldberg S D, Knorr K H, Blodau C, et al. 2010. Impact of altering the water table height of an acidic fen on N2O and NO fluxes and soil concentrations. Global Change Biology, 16(1): 220–233.CrossRefGoogle Scholar
  20. Harrison-Kirk T, Beare M H, Meenken E D, et al. 2013. Soil organic matter and texture affect responses to dry/wet cycles: Effects on carbon dioxide and nitrous oxide emissions. Soil Biology and Biochemistry, 57: 43–55.CrossRefGoogle Scholar
  21. Hernandez-Ramirez G, Brouder S M, Smith D R, et al. 2009. Greenhouse gas fluxes in an eastern corn belt soil: weather, nitrogen source, and rotation. Journal of Environmental Quallity, 38(3): 841–854.CrossRefGoogle Scholar
  22. Huxman T E, Snyder K A, Tissue D, et al. 2004a. Precipitation pulses and carbon fluxes in semiarid and arid ecosystems. Oecologia, 141(2): 254–268.Google Scholar
  23. Huxman T E, Smith M D, Fay P A, et al. 2004b. Convergence across biomes to a common rain-use efficiency. Nature, 429(10): 651–654.CrossRefGoogle Scholar
  24. Jin Z, Dong Y S, Qi Y C, et al. 2009. Precipitation pulses and soil CO2 emission in desert shrubland of Artemisia ordosica on the Ordos Plateau of Inner Mongolia, China. Pedosphere, 19(6): 799–807.CrossRefGoogle Scholar
  25. Kim D G, Mishurov M, Kiely G. 2010. Effect of increased N use and dry periods on N2O emission from a fertilized grassland. Nutrient Cycling in Agroecosystems, 88(3): 397–410.CrossRefGoogle Scholar
  26. Kim D G, Vargas R, Bond-Lamberty B, et al. 2012. Effects of soil rewetting and thawing on soil gas fluxes: a review of current literature and suggestions for future research. Biogeosciences, 9(7): 2459–2482.CrossRefGoogle Scholar
  27. Li Y, Barton L, Chen D L. 2011. Simulating response of N2O emissions to fertiliser N application and climatic variability from a rain-fed and wheatcropped soil in Western Australia. Journal of the Science of Food and Agriculture, 92(5): 1130–1143.CrossRefGoogle Scholar
  28. Liu X R, Dong Y S, Qi Y C, et al. 2010. N2O fluxes from the native and grazed semi-arid steppes and their driving factors in Inner Mongolia, China. Nutrient Cycling in Agroecosystems, 86(2): 231–240.CrossRefGoogle Scholar
  29. McSwiney C P, Robertson G P. 2005. Nonlinear response of N2O flux to incremental fertilizer addition in a continuous maize (Zea mays L.) cropping system. Global Change Biology, 11(10): 1712–1719.CrossRefGoogle Scholar
  30. Muhr J, Goldberg S D, Borken W, et al. 2008. Repeated drying-rewetting cycles and their effects on the emission of CO2, N2O, NO, and CH4 in a forest soil. Journal of Plant Nutrition and Soil Science, 171(5): 719–728.CrossRefGoogle Scholar
  31. Mummey D L, Smith J L, Bluhm G. 2000. Estimation of nitrous oxide emissions from US grasslands. Environmental Management, 25(2): 169–175.CrossRefGoogle Scholar
  32. Nobre A, Keller M, Crill P, et al. 2001. Short-term nitrous oxide profile dynamics and emissions response to water, nitrogen and carbon additions in two tropical soils. Biology and Fertility of Soils, 34(5): 363–373.CrossRefGoogle Scholar
  33. Peng Q, Qi Y C, Dong Y S, et al. 2011. Soil nitrous oxide emissions from a typical semiarid temperate steppe in inner Mongolia: effects of mineral nitrogen fertilizer levels and forms. Plant and Soil, 342(1): 345–357.CrossRefGoogle Scholar
  34. Priemé A, Christensen S. 2001. Natural perturbations, drying-wetting and freezing-thawing cycles, and the emission of nitrous oxide, carbon dioxide and methane from farmed organic soils. Soil Biology and Biochemistry, 33(15): 2083–2091.CrossRefGoogle Scholar
  35. Qi Y C, Dong Y S, Domroes M, et al. 2006. Comparison of CO2 effluxes and their driving factors between two temperate steppes in Inner Mongolia, China. Advances in Atmospheric Sciences, 23(5): 726–736.CrossRefGoogle Scholar
  36. Qi Y C, Dong Y S, Liu J Y, et al. 2007. Effect of the conversion of grassland to spring wheat field on the CO2 emission characteristics in Inner Mongolia, China. Soil and Tillage Research, 94(2): 310–320.CrossRefGoogle Scholar
  37. Rafique R, Hennessy D, Kiely G. 2011. Nitrous oxide emission from grazed grassland under different management systems. Ecosystems, 14(4): 563–582.CrossRefGoogle Scholar
  38. Rudaz A O, Davidson E A, Firestone M K. 1991. Sources of nitrous oxide production following wetting of dry soil. FEMS Microbiology Letters, 85(2): 117–124.CrossRefGoogle Scholar
  39. Saetre P, Stark J M. 2005. Microbial dynamics and carbon and nitrogen cycling following re-wetting of soils beneath two semi-arid plant species. Oecologia, 142(2): 247–260.CrossRefGoogle Scholar
  40. Schwinning S, Sala O E. 2004. Hierarchy of responses to resource pulses in arid and semi-arid ecosystems. Oecologia, 141(2): 211–220.Google Scholar
  41. Song L, Bao X, Liu X, et al. 2012. Impact of nitrogen addition on plant community in a semi-arid temperate steppe in China. Journal of Arid Land, 4(1): 3–10.CrossRefGoogle Scholar
  42. Sponseller R A. 2007. Precipitation pulses and soil CO2 flux in a Sonoran Desert ecosystem. Global Change Biology, 13(2): 426–436.CrossRefGoogle Scholar
  43. Trost B, Prochnow A, Drastig K, et al. 2013. Irrigation, soil organic carbon and N2O emissions. A review. Agronomy for Sustainable Development, 33(4): 733–749.CrossRefGoogle Scholar
  44. Van Haren J L M, Handley L L, Biel K Y, et al. 2005. Drought-induced nitrous oxide flux dynamics in an enclosed tropical forest. Global Change Biology, 11(8): 1247–1257.CrossRefGoogle Scholar
  45. Weier K L, Doran J W, Power J F, et al. 1993. Denitrification and the dinitrogen/nitrous oxide ratio as affected by soil water, available carbon, and nitrate. Science Society of America Journal, 57(1): 66–72.CrossRefGoogle Scholar
  46. Weitz A M, Linder E, Frolking S, et al. 2001. N2O emissions from humid tropical agricultural soils: effects of soil moisture, texture and nitrogen availability. Soil Biology and Biochemistry, 33(7–8): 1077–1093.CrossRefGoogle Scholar
  47. Wuebbles D J. 2009. Nitrous oxide: no laughing matter. Science, 326(2): 56–57.CrossRefGoogle Scholar
  48. Xu R, Wang Y S, Zheng X H, et al. 2003. A comparison between measured and modeled N2O emissions from Inner Mongolian semi-arid grassland. Plant and Soil, 255(2): 513–528.CrossRefGoogle Scholar
  49. Xu Z W, Wan S Q, Ren H Y, et al. 2012. Effects of water and nitrogen addition on species turnover in temperate grasslands in Northern China. PLOS ONE, 7(6): e39762. doi:10.1371/journal. pone.0039762.CrossRefGoogle Scholar
  50. Yao Z, Wu X, Wolf B, et al. 2010. Soil-atmosphere exchange potential of NO and N2O in different land use types of Inner Mongolia as affected by soil temperature, soil moisture, freeze-thaw, and drying-wetting events. Journal of Geophysical Research: Atmospheres, 115(D17): D17116. doi: 10.1029/2009JD013528.CrossRefGoogle Scholar
  51. Zhang F, Qi J, Li F M, et al. 2010. Quantifying nitrous oxide emissions from Chinese grasslands with a process-based model. Biogeosciences, 7(6): 2039–2050.CrossRefGoogle Scholar
  52. Zhang Y, He N, Zhang G, et al. 2013. Ammonia emissions from soil under sheep grazing in inner mongolian grasslands of China. Journal of Arid Land, 5(2): 155–165.CrossRefGoogle Scholar

Copyright information

© Science Press, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • XinChao Liu
    • 1
    • 2
  • YuChun Qi
    • 1
  • YunShe Dong
    • 1
    Email author
  • Qin Peng
    • 1
  • YaTing He
    • 1
    • 3
  • LiangJie Sun
    • 1
    • 2
  • JunQiang Jia
    • 1
    • 2
  • CongCong Cao
    • 1
    • 2
  1. 1.Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijingChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.Institute of Agricultural Resources and Regional PlanningCAASBeijingChina

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