Cell-Free eccDNAs: A New Type of Nucleic Acid Component for Liquid Biopsy?
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Extrachromosomal circular DNAs (eccDNAs) are circular DNAs that are originated from chromosomes, but are independent from chromosomal DNA. The eccDNAs are commonly found in various tissues and cell types, and in both normal and diseased conditions. Due to their highly heterogeneous origins and being widely spread in nearly all eukaryotes, the eccDNAs are believed to reflect the genome’s plasticity and instability. With the assistance of next-generation sequencing, more eccDNAs have been characterized at the molecular level. Recently, eccDNAs have been reported as cell-free DNAs in the circulation system. Importantly, these circulating eccDNAs have shown some evidence with disease associations, suggesting their potential utility as a new type of biomarker for disease detection, treatment assessment and progress surveillance. However, many challenges need to be addressed before implementing the eccDNAs as a new source of genetic material for liquid biopsy.
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Conflict of interest
The authors, JZ, SC, FZ and LW, disclose no potential conflicts of interest.
This study was partially supported by a National Institute of Health (R01CA212097) Grant to LW and National Natural Science Foundation of China (NSFC) (project# 81301752) and University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (project# UNPYSCT-2017056) Grants to JZ.
- 27.Kinoshita Y, Ohnishi N, Yamada Y, Kunisada T, Yamagishi H. Extrachromosomal circular DNA from nuclear fraction of higher plants. Plant Cell Physiol. 1985;26(7):1401–9.Google Scholar
- 37.Moller HD, Bojsen RK, Tachibana C, Parsons L, Botstein D, Regenberg B. Genome-wide purification of extrachromosomal circular DNA from eukaryotic cells. J Vis Exp. 2016;110:1–8.Google Scholar
- 53.Mouliere F, Piskorz AM, Chandrananda D, Moore E, Morris J, Smith CG, Goranova T, Heider K, Mair R, Supernat A, Gounaris I, Ros S, Wan JCM, Jimenez-Linan M, Gale D, Brindle K, Massie CE, Parkinson CA, Brenton JD, Rosenfeld N. Selecting short DNA fragments in plasma improves detection of circulating tumour DNA. bioRxiv. 2017. https://doi.org/10.1101/134437.CrossRefGoogle Scholar
- 55.Alekseeva LA, Mironova NL, Brenner EV, Kurilshikov AM, Patutina OA, Zenkova MA. Alteration of the exDNA profile in blood serum of LLC-bearing mice under the decrease of tumour invasion potential by bovine pancreatic DNase I treatment. PLoS One. 2017;12(2):e0171988.CrossRefPubMedPubMedCentralGoogle Scholar