Identification of a Novel Somatic Mutation Leading to Allele Dropout for EGFR L858R Genotyping in Non-Small Cell Lung Cancer
- 99 Downloads
While PCR-based genotyping methods abound in molecular testing for lung cancer therapy, these approaches may not provide the robust sensitivity to detect accurate genotypes in a variable cancer genomic background.
Here, we describe a study of a clinical tumor specimen containing a novel somatic single nucleotide variant that caused allele drop-out in EGFR L858R genotyping, resulting in a false-negative interpretation and impacting patient clinical management.
We demonstrate that a subsequent unbiased next-generation sequencing approach correctly identified the driver mutation, and therefore may be more reliable for somatic variant detection.
These findings magnify the potential pitfalls of PCR amplification-based approaches and stress the importance of unbiased and sensitive molecular testing strategies for therapeutic marker detection as molecular testing becomes the standard for determining clinical management of cancer patients.
KeywordsEpidermal Growth Factor Receptor Epidermal Growth Factor Receptor Mutation Epidermal Growth Factor Receptor Gene L858R Mutation Epidermal Growth Factor Receptor Mutation Status
Compliance with Ethical Standards
Conflict of interest
H. A. C. and J. L. Z. have no conflicts of interest to report, J.W.N. is a consultant for Clovis Oncology, CARET, Nektar, Boehringer Ingelheim, ARMO BioScience and ARIAD. C.D.B. is on the scientific advisory boards (SAB) of AncestryDNA, BigDataBio, Etalon DX, Liberty Biosecurity, and Personalis. He is also a founder and SAB chair of IdentifyGenomics. None of these entities played a role in the design, execution, interpretation, or presentation of this study.
This research was supported by grants from the National Institutes of Health (ClinGen 4U01HG007436-04 to C.D.B.; NCI 2PO1CA49605 to J.L.Z.).
- 1.Hankey BF, Ries LA, Edwards BK. The surveillance, epidemiology, and end results program: a national resource. Cancer Epidemiol Biomarkers Prev. 1999;8(12):1117.Google Scholar
- 16.Lindeman N, Cagle P, Beasley M, Chitale D, Dacic S, Giaccone G, et al. Molecular testing guideline for selection of lung cancer patients for EGFR and ALK tyrosine kinase inhibitors: guideline from the College of American Pathologists, International Association for the Study of Lung Cancer, and Association for Molecular Pathology. J Thorac Oncol. 2013;8(7):823–59.CrossRefPubMedPubMedCentralGoogle Scholar