Improving Energy Metabolism of Deproteinized Extract of Calf Blood Through Regulation of Hmgcs2, Cpt1a, Angptl4, Cyp8b1, and Ehhadh Genes in Mice

  • Tong Zhou
  • Guangyu Xu
  • Luyao Sun
  • Zhenxiang YuEmail author
  • Guixia WangEmail author


Herein, we described the physicochemical properties of deproteinized extract of calf blood(DECB) and established a hypoxia model treated with or without DECB to detect the sugar, lactic acid, protein, and ATP contents of mice and then identified and analyzed the differentially expressed genes between two groups using mRNA expression chip. According to the results of the airtight hypoxia experiment, mice in the model+DECB group had a significantly prolonged time of hypoxia tolerance compared with the model group. The biochemical test indicated that DECB could significantly increase the level of sugar, ATP and proteins and reduce the amount of lactic acid in mice. It also revealed that Hmgcs2, Cpt1a, Angptl4, Cyp8b1, and Ehhadh genes were involved in mice energy metabolism, and were closely associated with metabolic signaling pathway. These results suggest that DECB might be a potential drug to treat metabolic diseases. Among the genes with differential expression under hypoxia, Angptl4, Cyp8b1, and Ehhadh were critical factors for sugar metabolism. Hmgcs2 provided energy directly, and Cptla regulated cellular inflammatory responses, promoting energy metabolism.


Deproteinized extract of calf blood Energy metabolism mRNA chip 


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Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH 2019

Authors and Affiliations

  1. 1.Department of Endocrinology and Metabolismthe First Hospital of Jilin UniversityChangchunP. R. China
  2. 2.College of PharmacyBeihua UniversityJilinP. R. China
  3. 3.Department of Infectious Diseasesthe First Hospital of Jilin UniversityChangchunP. R. China
  4. 4.Department of Respirationthe First Hospital of Jilin UniversityChangchunP. R. China

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