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Improving Energy Metabolism of Deproteinized Extract of Calf Blood Through Regulation of Hmgcs2, Cpt1a, Angptl4, Cyp8b1, and Ehhadh Genes in Mice

  • Tong Zhou
  • Guangyu Xu
  • Luyao Sun
  • Zhenxiang YuEmail author
  • Guixia WangEmail author
Article
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Abstract

Herein, we described the physicochemical properties of deproteinized extract of calf blood(DECB) and established a hypoxia model treated with or without DECB to detect the sugar, lactic acid, protein, and ATP contents of mice and then identified and analyzed the differentially expressed genes between two groups using mRNA expression chip. According to the results of the airtight hypoxia experiment, mice in the model+DECB group had a significantly prolonged time of hypoxia tolerance compared with the model group. The biochemical test indicated that DECB could significantly increase the level of sugar, ATP and proteins and reduce the amount of lactic acid in mice. It also revealed that Hmgcs2, Cpt1a, Angptl4, Cyp8b1, and Ehhadh genes were involved in mice energy metabolism, and were closely associated with metabolic signaling pathway. These results suggest that DECB might be a potential drug to treat metabolic diseases. Among the genes with differential expression under hypoxia, Angptl4, Cyp8b1, and Ehhadh were critical factors for sugar metabolism. Hmgcs2 provided energy directly, and Cptla regulated cellular inflammatory responses, promoting energy metabolism.

Keywords

Deproteinized extract of calf blood Energy metabolism mRNA chip 

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References

  1. [1]
    Wu W., Zeng L. N., Peng Y. Y., Lu X. H., Li C. Y., Wang Z. C., European Review for Medical and Pharmacological Sciences, 2014, 18(22), 3406Google Scholar
  2. [2]
    Xu G. Y., Xu J. H., Han X., Li H. Y., Yuan G. X., An L. P., Du P. G., International Immunopharmacology, 2018, 56, 212CrossRefGoogle Scholar
  3. [3]
    Xu G. Y., Han X., Yuan G. X., An L. P., Du P. G., PLoS One, 2017, 12(7), e0180899CrossRefGoogle Scholar
  4. [4]
    Chen M. J., Gong L., Qiu X. D., Chinese Journal of Ophthalmology, 2012, 48(12), 1083Google Scholar
  5. [5]
    Fan C., Wang Y., Zhang Y., Lang L., Deng X., Cheng Y., Chinese Journal of Industrial Hygiene and Occupational Diseases, 2014, 32(12), 924Google Scholar
  6. [6]
    Huang D. W., Sherman B. T., Tan Q., Collins J. R., Alvord W. G., Roayaei J., Stephens R., Baseler M. W., Lane H. C., Lempicki R. A., Genome Biology, 2007, 8(9), R183CrossRefGoogle Scholar
  7. [7]
    Ashburner M., Ball C. A., Blake J. A., Botstein D., Butler H., Cherry J. M., Davis A. P., Dolinski K., Dwight S. S., Eppig J. T., Harris M. A., Hill D. P., Issel-Tarver L., Kasarskis A., Lewis S., Matese J. C., Richardson J. E., Ringwald M., Rubin G. M., Sherlock G., The Gene Ontology Consortium Nature Genetics, 2000, 25(1), 25CrossRefGoogle Scholar
  8. [8]
    Shi Q., Zhou W., Chen C., Zhang B. Y., Xiao K., Zhang X. C., Shen X. J., Li Q., Deng L. Q., Dong J. H., Lin W. Q., Huang P., Jiang W. J., Lv J., Han J., Dong X. P., PLoS One, 2015, 10(10), e0139552CrossRefGoogle Scholar
  9. [9]
    Irwin D. M., Tan H., Molecular Phylogenetics and Evolution, 2015, 70, 195CrossRefGoogle Scholar
  10. [10]
    Zhang T. T., Zhang G. M., Jin Y. H., Guo Y. X., Wang Z., Fan Y. X., El-Samahy M. A., Wang F., Tissue & Cell, 2017, 49(5), 603CrossRefGoogle Scholar
  11. [11]
    Helenius T. O., Misiorek J. O., Nystrom J. H., Fortelius L. E., Habte-zion A., Liao J., Asghar M. N., Zhang H., Azhar S., Omary M. B., Toivola D. M., Molecular Biology of the Cell, 2015, 26(12), 2298CrossRefGoogle Scholar
  12. [12]
    Kang D. Y., Nipin S. P., Darvin P., Joung Y. H., Byun H. J., Do C. H., Park K. D., Park M. N., Cho K. H., Yang Y. M., Animal Biotechnology, 2017, 28(3), 189CrossRefGoogle Scholar
  13. [13]
    Gobin S., Bonnefont J. P., Prip-Buus C., Mugnier C., Ferrec M., Demaugre F., Saudubray J. M., Rostane H., Djouadi F., Wilcox W., Cederbaum S., Haas R., Nyhan W. L., Green A., Gray G., Girard J., Thuillier L., Human Genetics, 2002, 111(2), 179CrossRefGoogle Scholar
  14. [14]
    Diaz-Rua R., Palou A., Oliver P., Food & Nutrition Research, 2016, 60, 33554CrossRefGoogle Scholar
  15. [15]
    Napal L., Marrero P. F., Haro D., Journal of Molecular Biology, 2005, 354(4), 751CrossRefGoogle Scholar
  16. [16]
    Gao X., Li K., Hui X., Kong X., Sweeney G., Wang Y., Xu A., Teng M., Liu P., Wu D., The Biochemical Journal, 2011, 435(3), 723CrossRefGoogle Scholar
  17. [17]
    Kim L., Kim H. G., Kim H., Kim H. H., Park S. K., Uhm C. S., Lee Z. H., Koh G. Y., Biochem. J., 2000, 346, 603CrossRefGoogle Scholar
  18. [18]
    Ito Y., Oike Y., Yasunaga K., Hamada K., Miyata K., Matsumoto S., Sugano S., Tanihara H., Masuho Y., Suda T., Cancer Research, 2003, 63(20), 6651Google Scholar
  19. [19]
    Janssen A. W. F., Dijk W., Boekhorst J., Kuipers F., Groen A. K., Lukovac S., Hooiveld G., Kersten S., Biochimica et Biophysica Acta, 2017, 1862(10), 1056CrossRefGoogle Scholar
  20. [20]
    Bertaggia E., Jensen K. K., Castro-Perez J., Xu Y., di Paolo G., Chan R. B., Wang L., Haeusler R. A., American Journal of Physiology Endocrinology and Metabolism, 2017, 313(2), E121CrossRefGoogle Scholar
  21. [21]
    Mork L. M., Strom S. C., Mode A., Ellis E. C., Journal of Clinical and Experimental Hepatology, 2016, 6(2), 87CrossRefGoogle Scholar
  22. [22]
    Watanabe M., Morimoto K., Houten S. M., Kaneko-Iwasaki N., Sugizaki T., Horai Y., Mataki C., Sato H., Murahashi K., Arita E., Schoonjans K., Suzuki T., Itoh H., Auwerx J., PLoS One, 2012, 7(8), e38286CrossRefGoogle Scholar
  23. [23]
    Houten S. M., Denis S., Argmann C. A., Jia Y., Ferdinandusse S., Reddy J. K., Wanders R. J., Journal of Lipid Research, 2012, 53(7), 1296CrossRefGoogle Scholar
  24. [24]
    Rosen M. B., Das K. P., Wood C. R., Wolf C. J., Abbott B. D., Lau C., Toxicology, 2013, 308, 129CrossRefGoogle Scholar

Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH 2019

Authors and Affiliations

  1. 1.Department of Endocrinology and Metabolismthe First Hospital of Jilin UniversityChangchunP. R. China
  2. 2.College of PharmacyBeihua UniversityJilinP. R. China
  3. 3.Department of Infectious Diseasesthe First Hospital of Jilin UniversityChangchunP. R. China
  4. 4.Department of Respirationthe First Hospital of Jilin UniversityChangchunP. R. China

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